Human respiratory syncytial virus vaccine antigen produced in plants
Human respiratory syncytial virus (RSV) is the primary cause of respiratory infection in infants worldwide. Currently there is no available vaccine, although studies in animal models have demonstrated protective immunity induced by an epitope of the RSV G‐protein representing amino acids 174–187. Tw...
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| Veröffentlicht in: | The FASEB journal 2000-11, Vol.14 (14), p.2323-2328 |
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| creator | Belanger, Helene Fleysh, Nina Cox, Shannon Hartman, Greg Deka, Deepali Trudel, Michel Koprowski, Hilary Yusibov, Vidadi |
| description | Human respiratory syncytial virus (RSV) is the primary cause of respiratory infection in infants worldwide. Currently there is no available vaccine, although studies in animal models have demonstrated protective immunity induced by an epitope of the RSV G‐protein representing amino acids 174–187. Two peptides containing amino acids 174–187 of the G‐protein of the human RSV A2 strain (NF1‐RSV/172–187 and NF2‐RSV/170–191) were separately engineered as translational fusions with the alfalfa mosaic virus coat protein and individually expressed in Nicotiana tabacum cv. Samsun NN plants through virus infection. RSV G‐protein peptides were expressed in infected plant tissues at significant levels within 2 wk of inoculation and purified as part of recombinant alfalfa mosaic virions. BALB/c mice immunized intraperitoneally with three doses of the purified recombinant viruses showed high levels of serum antibody specific for RSV G‐protein and were protected against infection with RSV Long strain.—Belanger, H., Fleysh, N., Cox, S., Bartman, G., Deka, D., Trudel, M., Koprowski, H., Yusibov, V. Human respiratory syncytial virus vaccine antigen produced in plants. FASEB J. 14, 2323–2328 (2000) |
| doi_str_mv | 10.1096/fj.00-0144com |
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Currently there is no available vaccine, although studies in animal models have demonstrated protective immunity induced by an epitope of the RSV G‐protein representing amino acids 174–187. Two peptides containing amino acids 174–187 of the G‐protein of the human RSV A2 strain (NF1‐RSV/172–187 and NF2‐RSV/170–191) were separately engineered as translational fusions with the alfalfa mosaic virus coat protein and individually expressed in Nicotiana tabacum cv. Samsun NN plants through virus infection. RSV G‐protein peptides were expressed in infected plant tissues at significant levels within 2 wk of inoculation and purified as part of recombinant alfalfa mosaic virions. BALB/c mice immunized intraperitoneally with three doses of the purified recombinant viruses showed high levels of serum antibody specific for RSV G‐protein and were protected against infection with RSV Long strain.—Belanger, H., Fleysh, N., Cox, S., Bartman, G., Deka, D., Trudel, M., Koprowski, H., Yusibov, V. Human respiratory syncytial virus vaccine antigen produced in plants. 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Currently there is no available vaccine, although studies in animal models have demonstrated protective immunity induced by an epitope of the RSV G‐protein representing amino acids 174–187. Two peptides containing amino acids 174–187 of the G‐protein of the human RSV A2 strain (NF1‐RSV/172–187 and NF2‐RSV/170–191) were separately engineered as translational fusions with the alfalfa mosaic virus coat protein and individually expressed in Nicotiana tabacum cv. Samsun NN plants through virus infection. RSV G‐protein peptides were expressed in infected plant tissues at significant levels within 2 wk of inoculation and purified as part of recombinant alfalfa mosaic virions. BALB/c mice immunized intraperitoneally with three doses of the purified recombinant viruses showed high levels of serum antibody specific for RSV G‐protein and were protected against infection with RSV Long strain.—Belanger, H., Fleysh, N., Cox, S., Bartman, G., Deka, D., Trudel, M., Koprowski, H., Yusibov, V. Human respiratory syncytial virus vaccine antigen produced in plants. FASEB J. 14, 2323–2328 (2000)</description><subject>AG protein</subject><subject>alfalfa mosaic virus</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antigens, Viral - genetics</subject><subject>Antigens, Viral - immunology</subject><subject>Antigens, Viral - ultrastructure</subject><subject>Base Sequence</subject><subject>DNA, Recombinant - genetics</subject><subject>Female</subject><subject>G protein</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - immunology</subject><subject>Nicotiana tabacum</subject><subject>Plants - genetics</subject><subject>Plants - immunology</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Toxic</subject><subject>respiratory syncytial virus</subject><subject>Respiratory Syncytial Virus, Human - genetics</subject><subject>Respiratory Syncytial Virus, Human - immunology</subject><subject>respiratory tract</subject><subject>RSV</subject><subject>Transfection</subject><subject>Vaccination</subject><subject>Viral Vaccines - genetics</subject><subject>Viral Vaccines - immunology</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAURS0EoqUwsqJMbCnPcezGbFAoBbXqAMyW6zwjV_kodlKUf0-qVmJjeldPR1dXh5BrCmMKUtzZzRggBpqmpi5PyJByBrHIBJySIWQyiYVg2YBchLABAApUnJMBpcBZwtMheZq3pa4ij2HrvG5q30Whq0zXOF1EO-fbEO20Ma7CSFeN-8Iq2vo6bw3mketz0X_DJTmzugh4dbwj8jl7_pjO48Xq5XX6sIhNCmwZY8K1SXSaSEQh1zlPUgNWWG0pt6itFIDW6lwyszbAacYECq0zxkQmuZZsRG4Pvf2E7xZDo0oXDBb9CKzboOhkwiRI3oPxATS-DsGjVVvvSu07RUHttSm7UQDqqK3nb47F7brE_I8-euqB-wPw4wrs_m9Ts_fHZPa2t52m09WS_QLQF3yD</recordid><startdate>200011</startdate><enddate>200011</enddate><creator>Belanger, Helene</creator><creator>Fleysh, Nina</creator><creator>Cox, Shannon</creator><creator>Hartman, Greg</creator><creator>Deka, Deepali</creator><creator>Trudel, Michel</creator><creator>Koprowski, Hilary</creator><creator>Yusibov, Vidadi</creator><general>Federation of American Societies for Experimental Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>200011</creationdate><title>Human respiratory syncytial virus vaccine antigen produced in plants</title><author>Belanger, Helene ; Fleysh, Nina ; Cox, Shannon ; Hartman, Greg ; Deka, Deepali ; Trudel, Michel ; Koprowski, Hilary ; Yusibov, Vidadi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403M-e25ac2a429ee69bd524c0f6faf15feaf960effad93cbc051836e6aa8336895a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>AG protein</topic><topic>alfalfa mosaic virus</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antigens, Viral - genetics</topic><topic>Antigens, Viral - immunology</topic><topic>Antigens, Viral - ultrastructure</topic><topic>Base Sequence</topic><topic>DNA, Recombinant - genetics</topic><topic>Female</topic><topic>G protein</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - immunology</topic><topic>Nicotiana tabacum</topic><topic>Plants - genetics</topic><topic>Plants - immunology</topic><topic>Plants, Genetically Modified</topic><topic>Plants, Toxic</topic><topic>respiratory syncytial virus</topic><topic>Respiratory Syncytial Virus, Human - genetics</topic><topic>Respiratory Syncytial Virus, Human - immunology</topic><topic>respiratory tract</topic><topic>RSV</topic><topic>Transfection</topic><topic>Vaccination</topic><topic>Viral Vaccines - genetics</topic><topic>Viral Vaccines - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belanger, Helene</creatorcontrib><creatorcontrib>Fleysh, Nina</creatorcontrib><creatorcontrib>Cox, Shannon</creatorcontrib><creatorcontrib>Hartman, Greg</creatorcontrib><creatorcontrib>Deka, Deepali</creatorcontrib><creatorcontrib>Trudel, Michel</creatorcontrib><creatorcontrib>Koprowski, Hilary</creatorcontrib><creatorcontrib>Yusibov, Vidadi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>The FASEB journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belanger, Helene</au><au>Fleysh, Nina</au><au>Cox, Shannon</au><au>Hartman, Greg</au><au>Deka, Deepali</au><au>Trudel, Michel</au><au>Koprowski, Hilary</au><au>Yusibov, Vidadi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human respiratory syncytial virus vaccine antigen produced in plants</atitle><jtitle>The FASEB journal</jtitle><addtitle>FASEB J</addtitle><date>2000-11</date><risdate>2000</risdate><volume>14</volume><issue>14</issue><spage>2323</spage><epage>2328</epage><pages>2323-2328</pages><issn>0892-6638</issn><eissn>1530-6860</eissn><abstract>Human respiratory syncytial virus (RSV) is the primary cause of respiratory infection in infants worldwide. Currently there is no available vaccine, although studies in animal models have demonstrated protective immunity induced by an epitope of the RSV G‐protein representing amino acids 174–187. Two peptides containing amino acids 174–187 of the G‐protein of the human RSV A2 strain (NF1‐RSV/172–187 and NF2‐RSV/170–191) were separately engineered as translational fusions with the alfalfa mosaic virus coat protein and individually expressed in Nicotiana tabacum cv. Samsun NN plants through virus infection. RSV G‐protein peptides were expressed in infected plant tissues at significant levels within 2 wk of inoculation and purified as part of recombinant alfalfa mosaic virions. BALB/c mice immunized intraperitoneally with three doses of the purified recombinant viruses showed high levels of serum antibody specific for RSV G‐protein and were protected against infection with RSV Long strain.—Belanger, H., Fleysh, N., Cox, S., Bartman, G., Deka, D., Trudel, M., Koprowski, H., Yusibov, V. Human respiratory syncytial virus vaccine antigen produced in plants. FASEB J. 14, 2323–2328 (2000)</abstract><cop>United States</cop><pub>Federation of American Societies for Experimental Biology</pub><pmid>11053254</pmid><doi>10.1096/fj.00-0144com</doi><tpages>6</tpages></addata></record> |
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| subjects | AG protein alfalfa mosaic virus Amino Acid Sequence Animals Antigens, Viral - genetics Antigens, Viral - immunology Antigens, Viral - ultrastructure Base Sequence DNA, Recombinant - genetics Female G protein Gene Expression Regulation Humans Mice Mice, Inbred BALB C Molecular Sequence Data Nicotiana - genetics Nicotiana - immunology Nicotiana tabacum Plants - genetics Plants - immunology Plants, Genetically Modified Plants, Toxic respiratory syncytial virus Respiratory Syncytial Virus, Human - genetics Respiratory Syncytial Virus, Human - immunology respiratory tract RSV Transfection Vaccination Viral Vaccines - genetics Viral Vaccines - immunology |
| title | Human respiratory syncytial virus vaccine antigen produced in plants |
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