Decreased level of osteopontin in children with allergic rhinitis during sublingual immunotherapy
Abstract Objective Sublingual immunotherapy (SLIT) is proven to be very effective in the treatment of allergic rhinitis (AR), but its regulatory mechanism and biomarkers for predicting efficacy are still unknown. Osteopontin (OPN), as a recently described Th2 inflammation related protein, plays key...
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| Veröffentlicht in: | International journal of pediatric otorhinolaryngology 2016-02, Vol.81, p.15-20 |
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| creator | Wang, Chunrui Wang, Kai Liu, Shaoting Qin, Xiaowei Chen, Kewa Zhang, Tianhong |
| description | Abstract Objective Sublingual immunotherapy (SLIT) is proven to be very effective in the treatment of allergic rhinitis (AR), but its regulatory mechanism and biomarkers for predicting efficacy are still unknown. Osteopontin (OPN), as a recently described Th2 inflammation related protein, plays key role in the pathogenesis of AR. The aim of this study was to identify the expression and role of OPN during SLIT in children. Methods Fifty house dust mite (HDM)-sensitized children with AR were enrolled in this study. AR children received HDM allergen extract or placebo for SLIT. Serum of different time points during treatment was collected and used for enzyme-linked immuno sorbent assay (ELISA) of OPN and related cytokines, respectively. Peripheral blood mononuclear cells from children after SLIT or placebo treatment were collected and stimulated with HDM with or without OPN/anti-OPN after one year's treatment. Results Our results showed that expression of OPN protein was decreased after one year's therapy. The decreased OPN expression was positively related to decreased Th2 cytokines and negatively related to enhanced IL-10 and TGF-β expression. In vitro experiments confirmed that children received SLIT treatment showed decreased production of Th2 cytokines by PBMCs after HDM stimulation. Conclusion During SLIT, decreased OPN expression was related to low Th2 cytokine expression and enhanced IL-10 and TGF-β expression. High serum OPN expression predicts poor treatment efficacy. OPN may be used as a biomarker for SLIT treatment. |
| doi_str_mv | 10.1016/j.ijporl.2015.12.001 |
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Osteopontin (OPN), as a recently described Th2 inflammation related protein, plays key role in the pathogenesis of AR. The aim of this study was to identify the expression and role of OPN during SLIT in children. Methods Fifty house dust mite (HDM)-sensitized children with AR were enrolled in this study. AR children received HDM allergen extract or placebo for SLIT. Serum of different time points during treatment was collected and used for enzyme-linked immuno sorbent assay (ELISA) of OPN and related cytokines, respectively. Peripheral blood mononuclear cells from children after SLIT or placebo treatment were collected and stimulated with HDM with or without OPN/anti-OPN after one year's treatment. Results Our results showed that expression of OPN protein was decreased after one year's therapy. The decreased OPN expression was positively related to decreased Th2 cytokines and negatively related to enhanced IL-10 and TGF-β expression. In vitro experiments confirmed that children received SLIT treatment showed decreased production of Th2 cytokines by PBMCs after HDM stimulation. Conclusion During SLIT, decreased OPN expression was related to low Th2 cytokine expression and enhanced IL-10 and TGF-β expression. High serum OPN expression predicts poor treatment efficacy. OPN may be used as a biomarker for SLIT treatment.</description><identifier>ISSN: 0165-5876</identifier><identifier>EISSN: 1872-8464</identifier><identifier>DOI: 10.1016/j.ijporl.2015.12.001</identifier><identifier>PMID: 26810282</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adolescent ; Allergens - immunology ; Allergic rhinitis ; Animals ; Biomarkers - blood ; Child ; Child, Preschool ; Cytokines - metabolism ; Dermatophagoides pteronyssinus ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - immunology ; Male ; Osteopontin ; Osteopontin - blood ; Otolaryngology ; Pediatrics ; Pyroglyphidae - immunology ; Rhinitis, Allergic - drug therapy ; Rhinitis, Allergic - immunology ; Sublingual immunotherapy ; Sublingual Immunotherapy - methods ; Th2 cytokines ; Treatment Outcome</subject><ispartof>International journal of pediatric otorhinolaryngology, 2016-02, Vol.81, p.15-20</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2015 Elsevier Ireland Ltd</rights><rights>Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-e2d7e34248793792e729b7ee16465b6feafa0194f6bf6914efde2c2812ef58043</citedby><cites>FETCH-LOGICAL-c450t-e2d7e34248793792e729b7ee16465b6feafa0194f6bf6914efde2c2812ef58043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijporl.2015.12.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26810282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Chunrui</creatorcontrib><creatorcontrib>Wang, Kai</creatorcontrib><creatorcontrib>Liu, Shaoting</creatorcontrib><creatorcontrib>Qin, Xiaowei</creatorcontrib><creatorcontrib>Chen, Kewa</creatorcontrib><creatorcontrib>Zhang, Tianhong</creatorcontrib><title>Decreased level of osteopontin in children with allergic rhinitis during sublingual immunotherapy</title><title>International journal of pediatric otorhinolaryngology</title><addtitle>Int J Pediatr Otorhinolaryngol</addtitle><description>Abstract Objective Sublingual immunotherapy (SLIT) is proven to be very effective in the treatment of allergic rhinitis (AR), but its regulatory mechanism and biomarkers for predicting efficacy are still unknown. Osteopontin (OPN), as a recently described Th2 inflammation related protein, plays key role in the pathogenesis of AR. The aim of this study was to identify the expression and role of OPN during SLIT in children. Methods Fifty house dust mite (HDM)-sensitized children with AR were enrolled in this study. AR children received HDM allergen extract or placebo for SLIT. Serum of different time points during treatment was collected and used for enzyme-linked immuno sorbent assay (ELISA) of OPN and related cytokines, respectively. Peripheral blood mononuclear cells from children after SLIT or placebo treatment were collected and stimulated with HDM with or without OPN/anti-OPN after one year's treatment. Results Our results showed that expression of OPN protein was decreased after one year's therapy. The decreased OPN expression was positively related to decreased Th2 cytokines and negatively related to enhanced IL-10 and TGF-β expression. In vitro experiments confirmed that children received SLIT treatment showed decreased production of Th2 cytokines by PBMCs after HDM stimulation. Conclusion During SLIT, decreased OPN expression was related to low Th2 cytokine expression and enhanced IL-10 and TGF-β expression. High serum OPN expression predicts poor treatment efficacy. OPN may be used as a biomarker for SLIT treatment.</description><subject>Adolescent</subject><subject>Allergens - immunology</subject><subject>Allergic rhinitis</subject><subject>Animals</subject><subject>Biomarkers - blood</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cytokines - metabolism</subject><subject>Dermatophagoides pteronyssinus</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Male</subject><subject>Osteopontin</subject><subject>Osteopontin - blood</subject><subject>Otolaryngology</subject><subject>Pediatrics</subject><subject>Pyroglyphidae - immunology</subject><subject>Rhinitis, Allergic - drug therapy</subject><subject>Rhinitis, Allergic - immunology</subject><subject>Sublingual immunotherapy</subject><subject>Sublingual Immunotherapy - methods</subject><subject>Th2 cytokines</subject><subject>Treatment Outcome</subject><issn>0165-5876</issn><issn>1872-8464</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2LFDEQhoMo7rj6D0Ry9NJtkslH90WQ9WMXFjyo55BOV3bSppM26V6Zf2-GWT14USioy1NV8LyF0EtKWkqofDO1flpSDi0jVLSUtYTQR2hHO8Wajkv-GO0qJhrRKXmBnpUyVUARIZ6iCyY7SljHdsi8B5vBFBhxgHsIODmcygppSXH1EdeyBx_GDBH_9OsBmxAg33mL88FHv_qCxy37eIfLNoTaNxOwn-ctpvUA2SzH5-iJM6HAi4d-ib59_PD16rq5_fzp5urdbWO5IGsDbFSw54x3qt-rnoFi_aAAqORSDNKBcYbQnjs5ONlTDm4EZllHGTjREb6_RK_Pe5ecfmxQVj37YiEEEyFtRVOl9j0hnKr_QCXpSc-lrCg_ozanUjI4vWQ_m3zUlOhTDnrS5xz0KQdNma6a69irhwvbMMP4Z-i3-Aq8PQNQldx7yLpYD9HC6DPYVY_J_-vC3wts1e-tCd_hCGVKW45Vt6a61AH95fQLp1egghDJqoRfQ_uxLg</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Wang, Chunrui</creator><creator>Wang, Kai</creator><creator>Liu, Shaoting</creator><creator>Qin, Xiaowei</creator><creator>Chen, Kewa</creator><creator>Zhang, Tianhong</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20160201</creationdate><title>Decreased level of osteopontin in children with allergic rhinitis during sublingual immunotherapy</title><author>Wang, Chunrui ; Wang, Kai ; Liu, Shaoting ; Qin, Xiaowei ; Chen, Kewa ; Zhang, Tianhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-e2d7e34248793792e729b7ee16465b6feafa0194f6bf6914efde2c2812ef58043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Allergens - immunology</topic><topic>Allergic rhinitis</topic><topic>Animals</topic><topic>Biomarkers - blood</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cytokines - metabolism</topic><topic>Dermatophagoides pteronyssinus</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Male</topic><topic>Osteopontin</topic><topic>Osteopontin - blood</topic><topic>Otolaryngology</topic><topic>Pediatrics</topic><topic>Pyroglyphidae - immunology</topic><topic>Rhinitis, Allergic - drug therapy</topic><topic>Rhinitis, Allergic - immunology</topic><topic>Sublingual immunotherapy</topic><topic>Sublingual Immunotherapy - methods</topic><topic>Th2 cytokines</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Chunrui</creatorcontrib><creatorcontrib>Wang, Kai</creatorcontrib><creatorcontrib>Liu, Shaoting</creatorcontrib><creatorcontrib>Qin, Xiaowei</creatorcontrib><creatorcontrib>Chen, Kewa</creatorcontrib><creatorcontrib>Zhang, Tianhong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International journal of pediatric otorhinolaryngology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Chunrui</au><au>Wang, Kai</au><au>Liu, Shaoting</au><au>Qin, Xiaowei</au><au>Chen, Kewa</au><au>Zhang, Tianhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased level of osteopontin in children with allergic rhinitis during sublingual immunotherapy</atitle><jtitle>International journal of pediatric otorhinolaryngology</jtitle><addtitle>Int J Pediatr Otorhinolaryngol</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>81</volume><spage>15</spage><epage>20</epage><pages>15-20</pages><issn>0165-5876</issn><eissn>1872-8464</eissn><abstract>Abstract Objective Sublingual immunotherapy (SLIT) is proven to be very effective in the treatment of allergic rhinitis (AR), but its regulatory mechanism and biomarkers for predicting efficacy are still unknown. Osteopontin (OPN), as a recently described Th2 inflammation related protein, plays key role in the pathogenesis of AR. The aim of this study was to identify the expression and role of OPN during SLIT in children. Methods Fifty house dust mite (HDM)-sensitized children with AR were enrolled in this study. AR children received HDM allergen extract or placebo for SLIT. Serum of different time points during treatment was collected and used for enzyme-linked immuno sorbent assay (ELISA) of OPN and related cytokines, respectively. Peripheral blood mononuclear cells from children after SLIT or placebo treatment were collected and stimulated with HDM with or without OPN/anti-OPN after one year's treatment. Results Our results showed that expression of OPN protein was decreased after one year's therapy. The decreased OPN expression was positively related to decreased Th2 cytokines and negatively related to enhanced IL-10 and TGF-β expression. In vitro experiments confirmed that children received SLIT treatment showed decreased production of Th2 cytokines by PBMCs after HDM stimulation. Conclusion During SLIT, decreased OPN expression was related to low Th2 cytokine expression and enhanced IL-10 and TGF-β expression. High serum OPN expression predicts poor treatment efficacy. OPN may be used as a biomarker for SLIT treatment.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>26810282</pmid><doi>10.1016/j.ijporl.2015.12.001</doi><tpages>6</tpages></addata></record> |
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| subjects | Adolescent Allergens - immunology Allergic rhinitis Animals Biomarkers - blood Child Child, Preschool Cytokines - metabolism Dermatophagoides pteronyssinus Enzyme-Linked Immunosorbent Assay Female Humans Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - immunology Male Osteopontin Osteopontin - blood Otolaryngology Pediatrics Pyroglyphidae - immunology Rhinitis, Allergic - drug therapy Rhinitis, Allergic - immunology Sublingual immunotherapy Sublingual Immunotherapy - methods Th2 cytokines Treatment Outcome |
| title | Decreased level of osteopontin in children with allergic rhinitis during sublingual immunotherapy |
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