Recombinant phospholipase A sub(1) of the outer membrane of psychrotrophic Yersinia pseudotuberculosis: Expression, purification, and characterization

The pldA gene encoding membrane-bound phospholipase A sub(1) of Yersinia pseudotuberculosis was cloned and expressed in Escherichia coli cells. Recombinant phospholipase A sub(1) (rPldA) was isolated from inclusion bodies dissolved in 8 M urea by two-stage chromatography (ion-exchange and gel-filtra...

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Veröffentlicht in:	Biochemistry (Moscow) 2016-01, Vol.81 (1), p.47-57
Hauptverfasser:	Bakholdina, SI, Tischenko, N M, Sidorin, E V, Isaeva, M P, Likhatskaya, G N, Dmitrenok, P S, Kim, NYu, Chernikov, O V, Solov'eva, T F
Format:	Artikel
Sprache:	eng
Schlagworte:	Escherichia coli Yersinia pseudotuberculosis
Online-Zugang:	Volltext
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