Discovery of keratinases using bacteria isolated from marine environments
Bacteria are important for the biodegradation of keratin. Thus, a workflow to isolate keratin-degrading bacteria utilizing an optimized azo-keratin assay was established. Deteriorated feather samples, collected in marine shoreline environments from the intertidal zone, yielded 50 unique bacterial is...
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| Veröffentlicht in: | Systematic and applied microbiology 2016-02, Vol.39 (1), p.49-57 |
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| creator | Herzog, Bastian Overy, David P. Haltli, Bradley Kerr, Russell G. |
| description | Bacteria are important for the biodegradation of keratin. Thus, a workflow to isolate keratin-degrading bacteria utilizing an optimized azo-keratin assay was established. Deteriorated feather samples, collected in marine shoreline environments from the intertidal zone, yielded 50 unique bacterial isolates exhibiting keratin degradation when feather meal was supplied as keratin substrate. The majority of isolates, identified by 16S sequencing, belonged to genera previously reported to produce keratinases: Bacillus spp. (42%) and Stenotrophomonas spp. (40%). The remaining 18% represented the genera Alcaligenes, Chryseobacterium, Salinivibrio, Delftia, Stappia, and Microbacterium, genera not previously been associated with keratinase production. The workflow, also applied to 21 Bacilli from our in-house culture collection, additionally revealed four Bacilli with remarkable feather degradation potential. The industrial applicability of their associated keratinases was evaluated and the most active keratinase expressed in E. coli to confirm keratinase expression. Enriched keratinase fractions demonstrated activity up to 75°C and retained viability when stored lyophilized at 20°C for up to 200d. |
| doi_str_mv | 10.1016/j.syapm.2015.10.004 |
| format | Article |
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Thus, a workflow to isolate keratin-degrading bacteria utilizing an optimized azo-keratin assay was established. Deteriorated feather samples, collected in marine shoreline environments from the intertidal zone, yielded 50 unique bacterial isolates exhibiting keratin degradation when feather meal was supplied as keratin substrate. The majority of isolates, identified by 16S sequencing, belonged to genera previously reported to produce keratinases: Bacillus spp. (42%) and Stenotrophomonas spp. (40%). The remaining 18% represented the genera Alcaligenes, Chryseobacterium, Salinivibrio, Delftia, Stappia, and Microbacterium, genera not previously been associated with keratinase production. The workflow, also applied to 21 Bacilli from our in-house culture collection, additionally revealed four Bacilli with remarkable feather degradation potential. The industrial applicability of their associated keratinases was evaluated and the most active keratinase expressed in E. coli to confirm keratinase expression. Enriched keratinase fractions demonstrated activity up to 75°C and retained viability when stored lyophilized at 20°C for up to 200d.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1016/j.syapm.2015.10.004</identifier><identifier>PMID: 26607323</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject><![CDATA[Alcaligenes ; Alcaligenes - isolation & purification ; Alcaligenes - metabolism ; Animals ; Aquatic Organisms - isolation & purification ; Aquatic Organisms - metabolism ; Azo-keratin ; Bacillus ; Bacillus - isolation & purification ; Bacillus - metabolism ; Biodegradation ; Biodegradation, Environmental ; Chryseobacterium ; Chryseobacterium - isolation & purification ; Chryseobacterium - metabolism ; Delftia - isolation & purification ; Delftia - metabolism ; Environmental isolates ; Escherichia coli ; Feather meal ; Feathers - metabolism ; Feathers - microbiology ; Industrial enzymes ; Keratins - metabolism ; Microbacterium ; Peptide Hydrolases - metabolism ; Stappia ; Stenotrophomonas ; Stenotrophomonas - isolation & purification ; Stenotrophomonas - metabolism]]></subject><ispartof>Systematic and applied microbiology, 2016-02, Vol.39 (1), p.49-57</ispartof><rights>2015 Elsevier GmbH</rights><rights>Copyright © 2015 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-be30c5f19f5861f59d94938f2547621a6a8158bf5d36cb91c1edf8b409352b983</citedby><cites>FETCH-LOGICAL-c462t-be30c5f19f5861f59d94938f2547621a6a8158bf5d36cb91c1edf8b409352b983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.syapm.2015.10.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26607323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Herzog, Bastian</creatorcontrib><creatorcontrib>Overy, David P.</creatorcontrib><creatorcontrib>Haltli, Bradley</creatorcontrib><creatorcontrib>Kerr, Russell G.</creatorcontrib><title>Discovery of keratinases using bacteria isolated from marine environments</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>Bacteria are important for the biodegradation of keratin. Thus, a workflow to isolate keratin-degrading bacteria utilizing an optimized azo-keratin assay was established. Deteriorated feather samples, collected in marine shoreline environments from the intertidal zone, yielded 50 unique bacterial isolates exhibiting keratin degradation when feather meal was supplied as keratin substrate. The majority of isolates, identified by 16S sequencing, belonged to genera previously reported to produce keratinases: Bacillus spp. (42%) and Stenotrophomonas spp. (40%). The remaining 18% represented the genera Alcaligenes, Chryseobacterium, Salinivibrio, Delftia, Stappia, and Microbacterium, genera not previously been associated with keratinase production. The workflow, also applied to 21 Bacilli from our in-house culture collection, additionally revealed four Bacilli with remarkable feather degradation potential. The industrial applicability of their associated keratinases was evaluated and the most active keratinase expressed in E. coli to confirm keratinase expression. Enriched keratinase fractions demonstrated activity up to 75°C and retained viability when stored lyophilized at 20°C for up to 200d.</description><subject>Alcaligenes</subject><subject>Alcaligenes - isolation & purification</subject><subject>Alcaligenes - metabolism</subject><subject>Animals</subject><subject>Aquatic Organisms - isolation & purification</subject><subject>Aquatic Organisms - metabolism</subject><subject>Azo-keratin</subject><subject>Bacillus</subject><subject>Bacillus - isolation & purification</subject><subject>Bacillus - metabolism</subject><subject>Biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Chryseobacterium</subject><subject>Chryseobacterium - isolation & purification</subject><subject>Chryseobacterium - metabolism</subject><subject>Delftia - isolation & purification</subject><subject>Delftia - metabolism</subject><subject>Environmental isolates</subject><subject>Escherichia coli</subject><subject>Feather meal</subject><subject>Feathers - metabolism</subject><subject>Feathers - microbiology</subject><subject>Industrial enzymes</subject><subject>Keratins - metabolism</subject><subject>Microbacterium</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Stappia</subject><subject>Stenotrophomonas</subject><subject>Stenotrophomonas - isolation & purification</subject><subject>Stenotrophomonas - metabolism</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1rGzEQhkVoiJ2PX1Aoe-xlXY2-Vjr0UNwmNQR6Sc5Cqx0Fud6VK60N_vdZx06PpaeB4Zl5eR9CPgJdAAX1Zb0oB7ftF4yCnDYLSsUFmYMCXVOjxQcypw3jNaOMzsh1KWtKQRgFV2TGlKINZ3xOVt9j8WmP-VClUP3G7MY4uIKl2pU4vFSt8yPm6KpY0saN2FUhp77qXY4DVjjsY05Dj8NYbsllcJuCd-d5Q57vfzwtf9aPvx5Wy2-PtReKjXWLnHoZwASpFQRpOiMM14FJ0SgGTjkNUrdBdlz51oAH7IJuBTVcstZofkM-n_5uc_qzwzLafqqAm40bMO2KhabhmhndwH-gSnCuhWQTyk-oz6mUjMFuc5xaHixQe9Rt1_ZNtz3qPi4n3dPVp3PAru2x-3vz7ncCvp4AnIzsI2ZbfMTBYxcz-tF2Kf4z4BVPp5GW</recordid><startdate>201602</startdate><enddate>201602</enddate><creator>Herzog, Bastian</creator><creator>Overy, David P.</creator><creator>Haltli, Bradley</creator><creator>Kerr, Russell G.</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7TN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>201602</creationdate><title>Discovery of keratinases using bacteria isolated from marine environments</title><author>Herzog, Bastian ; 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Thus, a workflow to isolate keratin-degrading bacteria utilizing an optimized azo-keratin assay was established. Deteriorated feather samples, collected in marine shoreline environments from the intertidal zone, yielded 50 unique bacterial isolates exhibiting keratin degradation when feather meal was supplied as keratin substrate. The majority of isolates, identified by 16S sequencing, belonged to genera previously reported to produce keratinases: Bacillus spp. (42%) and Stenotrophomonas spp. (40%). The remaining 18% represented the genera Alcaligenes, Chryseobacterium, Salinivibrio, Delftia, Stappia, and Microbacterium, genera not previously been associated with keratinase production. The workflow, also applied to 21 Bacilli from our in-house culture collection, additionally revealed four Bacilli with remarkable feather degradation potential. 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| subjects | Alcaligenes Alcaligenes - isolation & purification Alcaligenes - metabolism Animals Aquatic Organisms - isolation & purification Aquatic Organisms - metabolism Azo-keratin Bacillus Bacillus - isolation & purification Bacillus - metabolism Biodegradation Biodegradation, Environmental Chryseobacterium Chryseobacterium - isolation & purification Chryseobacterium - metabolism Delftia - isolation & purification Delftia - metabolism Environmental isolates Escherichia coli Feather meal Feathers - metabolism Feathers - microbiology Industrial enzymes Keratins - metabolism Microbacterium Peptide Hydrolases - metabolism Stappia Stenotrophomonas Stenotrophomonas - isolation & purification Stenotrophomonas - metabolism |
| title | Discovery of keratinases using bacteria isolated from marine environments |
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