DNA methylation at selected CpG sites in peripheral blood leukocytes is predictive of gastric cancer
Recently, a set of studies addressed the question of the prevalence of aberrant methylation in surrogate tissues, such as peripheral blood leukocytes. Toward this aim, we conducted a case-control pilot study to investigate aberrant methylation in leukocytes of gastric cancer patients. The SNuPE comb...
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| Veröffentlicht in: | Anticancer research 2014-10, Vol.34 (10), p.5381-5388 |
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| container_title | Anticancer research |
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| creator | Dauksa, Albertas Gulbinas, Antanas Endzinas, Zilvinas Oldenburg, Johannes El-Maarri, Osman |
| description | Recently, a set of studies addressed the question of the prevalence of aberrant methylation in surrogate tissues, such as peripheral blood leukocytes. Toward this aim, we conducted a case-control pilot study to investigate aberrant methylation in leukocytes of gastric cancer patients.
The SNuPE combined with ion pair reverse phase HPLC (SIRPH method) was used to examine site-specific methylation status at selected CpG sites of the promoter regions of APC, ACIN1, BCL2, CD44, DAPK1, CDKN2A, RARB, TNFRSF10C HS3ST2 and of LINE-1, Alu repeats.
We observed that in the patients, tumor suppressor genes were slightly but significantly higher methylated at several CpG sites, while DNA repetitive elements were slightly less methylated compared to controls. This was found to be significantly associated with higher prevalence for gastric cancer.
These results suggest that larger studies must be carried-out to explore the biological significance and clinical usefulness of leukocyte DNA as non-invasive detection tool for gastric cancer. |
| format | Article |
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The SNuPE combined with ion pair reverse phase HPLC (SIRPH method) was used to examine site-specific methylation status at selected CpG sites of the promoter regions of APC, ACIN1, BCL2, CD44, DAPK1, CDKN2A, RARB, TNFRSF10C HS3ST2 and of LINE-1, Alu repeats.
We observed that in the patients, tumor suppressor genes were slightly but significantly higher methylated at several CpG sites, while DNA repetitive elements were slightly less methylated compared to controls. This was found to be significantly associated with higher prevalence for gastric cancer.
These results suggest that larger studies must be carried-out to explore the biological significance and clinical usefulness of leukocyte DNA as non-invasive detection tool for gastric cancer.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 25275032</identifier><language>eng</language><publisher>Greece</publisher><subject>Aged ; Aged, 80 and over ; Case-Control Studies ; Cluster Analysis ; CpG Islands ; DNA Methylation ; Epigenesis, Genetic ; Epigenomics ; Female ; Gene Expression Profiling ; Humans ; Leukocytes - metabolism ; Male ; Middle Aged ; Neoplasm Grading ; Neoplasm Metastasis ; Neoplasm Staging ; Prevalence ; Repetitive Sequences, Nucleic Acid ; Stomach Neoplasms - epidemiology ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology</subject><ispartof>Anticancer research, 2014-10, Vol.34 (10), p.5381-5388</ispartof><rights>Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25275032$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dauksa, Albertas</creatorcontrib><creatorcontrib>Gulbinas, Antanas</creatorcontrib><creatorcontrib>Endzinas, Zilvinas</creatorcontrib><creatorcontrib>Oldenburg, Johannes</creatorcontrib><creatorcontrib>El-Maarri, Osman</creatorcontrib><title>DNA methylation at selected CpG sites in peripheral blood leukocytes is predictive of gastric cancer</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Recently, a set of studies addressed the question of the prevalence of aberrant methylation in surrogate tissues, such as peripheral blood leukocytes. Toward this aim, we conducted a case-control pilot study to investigate aberrant methylation in leukocytes of gastric cancer patients.
The SNuPE combined with ion pair reverse phase HPLC (SIRPH method) was used to examine site-specific methylation status at selected CpG sites of the promoter regions of APC, ACIN1, BCL2, CD44, DAPK1, CDKN2A, RARB, TNFRSF10C HS3ST2 and of LINE-1, Alu repeats.
We observed that in the patients, tumor suppressor genes were slightly but significantly higher methylated at several CpG sites, while DNA repetitive elements were slightly less methylated compared to controls. This was found to be significantly associated with higher prevalence for gastric cancer.
These results suggest that larger studies must be carried-out to explore the biological significance and clinical usefulness of leukocyte DNA as non-invasive detection tool for gastric cancer.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Case-Control Studies</subject><subject>Cluster Analysis</subject><subject>CpG Islands</subject><subject>DNA Methylation</subject><subject>Epigenesis, Genetic</subject><subject>Epigenomics</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Leukocytes - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Grading</subject><subject>Neoplasm Metastasis</subject><subject>Neoplasm Staging</subject><subject>Prevalence</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Stomach Neoplasms - epidemiology</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1OwzAYRS0EoqXwCsgjSyT_OxmrAgWpggXmyLE_U4PTBNtB6ttTQZkZrs5wj-5wT9Cc6oZWWnJyiuaESVJpQuQMXeT8TohSTc3P0YxJpiXhbI7c7dMS91C2-2hKGHbYFJwhgi3g8Gpc4xwKZBx2eIQUxi0kE3EXh8HhCNPHYPc_dcZjAhdsCV-AB4_fTC4pWGzNzkK6RGfexAxXRy7Q6_3dy-qh2jyvH1fLTTUyIUrlOkFBdA60ctJyo2pqiCCHKICmVh011GtCO0etENJo7wU0XlPPfO0M4wt087s7puFzglzaPmQLMZodDFNuqda8Zkoz_r8qa0WaRnF5UK-P6tT14Noxhd6kfft3Iv8G711vYw</recordid><startdate>201410</startdate><enddate>201410</enddate><creator>Dauksa, Albertas</creator><creator>Gulbinas, Antanas</creator><creator>Endzinas, Zilvinas</creator><creator>Oldenburg, Johannes</creator><creator>El-Maarri, Osman</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7T5</scope><scope>7TM</scope><scope>H94</scope></search><sort><creationdate>201410</creationdate><title>DNA methylation at selected CpG sites in peripheral blood leukocytes is predictive of gastric cancer</title><author>Dauksa, Albertas ; Gulbinas, Antanas ; Endzinas, Zilvinas ; Oldenburg, Johannes ; El-Maarri, Osman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p244t-db41e4bde76d5c3a681a040a046ee986b1a1f701bd1c445a7ff4e9f71f2f8da23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Case-Control Studies</topic><topic>Cluster Analysis</topic><topic>CpG Islands</topic><topic>DNA Methylation</topic><topic>Epigenesis, Genetic</topic><topic>Epigenomics</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Leukocytes - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm Grading</topic><topic>Neoplasm Metastasis</topic><topic>Neoplasm Staging</topic><topic>Prevalence</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Stomach Neoplasms - epidemiology</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dauksa, Albertas</creatorcontrib><creatorcontrib>Gulbinas, Antanas</creatorcontrib><creatorcontrib>Endzinas, Zilvinas</creatorcontrib><creatorcontrib>Oldenburg, Johannes</creatorcontrib><creatorcontrib>El-Maarri, Osman</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dauksa, Albertas</au><au>Gulbinas, Antanas</au><au>Endzinas, Zilvinas</au><au>Oldenburg, Johannes</au><au>El-Maarri, Osman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA methylation at selected CpG sites in peripheral blood leukocytes is predictive of gastric cancer</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2014-10</date><risdate>2014</risdate><volume>34</volume><issue>10</issue><spage>5381</spage><epage>5388</epage><pages>5381-5388</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Recently, a set of studies addressed the question of the prevalence of aberrant methylation in surrogate tissues, such as peripheral blood leukocytes. Toward this aim, we conducted a case-control pilot study to investigate aberrant methylation in leukocytes of gastric cancer patients.
The SNuPE combined with ion pair reverse phase HPLC (SIRPH method) was used to examine site-specific methylation status at selected CpG sites of the promoter regions of APC, ACIN1, BCL2, CD44, DAPK1, CDKN2A, RARB, TNFRSF10C HS3ST2 and of LINE-1, Alu repeats.
We observed that in the patients, tumor suppressor genes were slightly but significantly higher methylated at several CpG sites, while DNA repetitive elements were slightly less methylated compared to controls. This was found to be significantly associated with higher prevalence for gastric cancer.
These results suggest that larger studies must be carried-out to explore the biological significance and clinical usefulness of leukocyte DNA as non-invasive detection tool for gastric cancer.</abstract><cop>Greece</cop><pmid>25275032</pmid><tpages>8</tpages></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Aged Aged, 80 and over Case-Control Studies Cluster Analysis CpG Islands DNA Methylation Epigenesis, Genetic Epigenomics Female Gene Expression Profiling Humans Leukocytes - metabolism Male Middle Aged Neoplasm Grading Neoplasm Metastasis Neoplasm Staging Prevalence Repetitive Sequences, Nucleic Acid Stomach Neoplasms - epidemiology Stomach Neoplasms - genetics Stomach Neoplasms - metabolism Stomach Neoplasms - pathology |
| title | DNA methylation at selected CpG sites in peripheral blood leukocytes is predictive of gastric cancer |
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