DOT1L histone methyltransferase regulates the expression of BCAT1 and is involved in sphere formation and cell migration of breast cancer cell lines
DOT1L is a histone H3 lysine 79 (H3K79) methyltransferase mainly implicated in leukemia. Here we analyzed the function of DOT1L in breast cancer cells. The expression of DOT1L was up-regulated in malignant breast cancer tissues. Over-expression of DOT1L significantly increased the sphere formation a...
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| Veröffentlicht in: | Biochimie 2016-04, Vol.123, p.20-31 |
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| creator | Oktyabri, Dulamsuren Ishimura, Akihiko Tange, Shoichiro Terashima, Minoru Suzuki, Takeshi |
| description | DOT1L is a histone H3 lysine 79 (H3K79) methyltransferase mainly implicated in leukemia. Here we analyzed the function of DOT1L in breast cancer cells. The expression of DOT1L was up-regulated in malignant breast cancer tissues. Over-expression of DOT1L significantly increased the sphere formation and the cell migration activities of MCF7 breast cancer cell line. In contrast, knockdown of DOT1L reduced the cell migration activity of MDA-MB-231 breast cancer cell line. BCAT1, which encodes a branched-chain amino acid transaminase, was identified as one of the target genes controlled by DOT1L through the regulation of H3K79 methylation. Mechanistic investigation revealed that BCAT1 might be an important regulator responsible for DOT1L-mediated sphere formation and cell migration in breast cancer cells.
•DOT1L histone methyltransferase is up-regulated in malignant breast cancers.•DOT1L is involved in sphere formation and cell migration of breast cancer cell lines.•DOT1L regulates the expression of BCAT1 through histone H3K79 methylation.•BCAT1 is responsible for DOT1L-mediated cell migration and sphere formation. |
| doi_str_mv | 10.1016/j.biochi.2016.01.005 |
| format | Article |
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•DOT1L histone methyltransferase is up-regulated in malignant breast cancers.•DOT1L is involved in sphere formation and cell migration of breast cancer cell lines.•DOT1L regulates the expression of BCAT1 through histone H3K79 methylation.•BCAT1 is responsible for DOT1L-mediated cell migration and sphere formation.</description><identifier>ISSN: 0300-9084</identifier><identifier>EISSN: 1638-6183</identifier><identifier>DOI: 10.1016/j.biochi.2016.01.005</identifier><identifier>PMID: 26783998</identifier><language>eng</language><publisher>France: Elsevier B.V</publisher><subject>Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Cancer progression ; Cell Line, Tumor ; Cell migration ; Female ; Gene Expression Regulation ; Histone methyltransferase ; Humans ; Methylation ; Methyltransferases - metabolism ; Neoplasm Metastasis ; Sphere formation ; Transaminases - genetics ; Transcriptional regulation</subject><ispartof>Biochimie, 2016-04, Vol.123, p.20-31</ispartof><rights>2016 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM)</rights><rights>Copyright © 2016 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-c0172231c8b8a882f8754de181608ecfa82e1e82ad1513a61d3bd375fafbba573</citedby><cites>FETCH-LOGICAL-c428t-c0172231c8b8a882f8754de181608ecfa82e1e82ad1513a61d3bd375fafbba573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0300908416000158$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26783998$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oktyabri, Dulamsuren</creatorcontrib><creatorcontrib>Ishimura, Akihiko</creatorcontrib><creatorcontrib>Tange, Shoichiro</creatorcontrib><creatorcontrib>Terashima, Minoru</creatorcontrib><creatorcontrib>Suzuki, Takeshi</creatorcontrib><title>DOT1L histone methyltransferase regulates the expression of BCAT1 and is involved in sphere formation and cell migration of breast cancer cell lines</title><title>Biochimie</title><addtitle>Biochimie</addtitle><description>DOT1L is a histone H3 lysine 79 (H3K79) methyltransferase mainly implicated in leukemia. Here we analyzed the function of DOT1L in breast cancer cells. The expression of DOT1L was up-regulated in malignant breast cancer tissues. Over-expression of DOT1L significantly increased the sphere formation and the cell migration activities of MCF7 breast cancer cell line. In contrast, knockdown of DOT1L reduced the cell migration activity of MDA-MB-231 breast cancer cell line. BCAT1, which encodes a branched-chain amino acid transaminase, was identified as one of the target genes controlled by DOT1L through the regulation of H3K79 methylation. Mechanistic investigation revealed that BCAT1 might be an important regulator responsible for DOT1L-mediated sphere formation and cell migration in breast cancer cells.
•DOT1L histone methyltransferase is up-regulated in malignant breast cancers.•DOT1L is involved in sphere formation and cell migration of breast cancer cell lines.•DOT1L regulates the expression of BCAT1 through histone H3K79 methylation.•BCAT1 is responsible for DOT1L-mediated cell migration and sphere formation.</description><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Cancer progression</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Histone methyltransferase</subject><subject>Humans</subject><subject>Methylation</subject><subject>Methyltransferases - metabolism</subject><subject>Neoplasm Metastasis</subject><subject>Sphere formation</subject><subject>Transaminases - genetics</subject><subject>Transcriptional regulation</subject><issn>0300-9084</issn><issn>1638-6183</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9u1DAQxi0Eots_b4CQj1wSPPEm8V6QygIFaaVetmfLcSaNV0m8eLyr9j144DpK4chpPOPfN2PPx9gHEDkIqD4f8sZ527u8SFkuIBeifMNWUEmVVaDkW7YSUohsI9T6gl0SHUQiRLF5zy6KqlZys1Er9ufb_R52vHcU_YR8xNg_DzGYiToMhpAHfDwNJiLx2CPHp2NAIucn7jv-dXu7B26mljvibjr74YzpPHE69hiQdz6MJs7wzFgcBj66x7CUkr4JaChyayaLYbkf3IR0zd51ZiC8eY1X7OHH9_32Z7a7v_u1vd1ldl2omFkBdVFIsKpRRqmiU3W5bhEUVEKh7YwqEFAVpoUSpKmglU0r67IzXdOYspZX7NPS9xj87xNS1KOj-RlmQn8iDXUtlVC1rBK6XlAbPFHATh-DG0141iD07Ic-6MUPPfuhBei07ST7-Drh1IzY_hP9NSABXxYA0z_PDoMm6zDto3UBbdStd_-f8AKD2Z_x</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Oktyabri, Dulamsuren</creator><creator>Ishimura, Akihiko</creator><creator>Tange, Shoichiro</creator><creator>Terashima, Minoru</creator><creator>Suzuki, Takeshi</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201604</creationdate><title>DOT1L histone methyltransferase regulates the expression of BCAT1 and is involved in sphere formation and cell migration of breast cancer cell lines</title><author>Oktyabri, Dulamsuren ; Ishimura, Akihiko ; Tange, Shoichiro ; Terashima, Minoru ; Suzuki, Takeshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-c0172231c8b8a882f8754de181608ecfa82e1e82ad1513a61d3bd375fafbba573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Cancer progression</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Histone methyltransferase</topic><topic>Humans</topic><topic>Methylation</topic><topic>Methyltransferases - metabolism</topic><topic>Neoplasm Metastasis</topic><topic>Sphere formation</topic><topic>Transaminases - genetics</topic><topic>Transcriptional regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oktyabri, Dulamsuren</creatorcontrib><creatorcontrib>Ishimura, Akihiko</creatorcontrib><creatorcontrib>Tange, Shoichiro</creatorcontrib><creatorcontrib>Terashima, Minoru</creatorcontrib><creatorcontrib>Suzuki, Takeshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oktyabri, Dulamsuren</au><au>Ishimura, Akihiko</au><au>Tange, Shoichiro</au><au>Terashima, Minoru</au><au>Suzuki, Takeshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DOT1L histone methyltransferase regulates the expression of BCAT1 and is involved in sphere formation and cell migration of breast cancer cell lines</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>2016-04</date><risdate>2016</risdate><volume>123</volume><spage>20</spage><epage>31</epage><pages>20-31</pages><issn>0300-9084</issn><eissn>1638-6183</eissn><abstract>DOT1L is a histone H3 lysine 79 (H3K79) methyltransferase mainly implicated in leukemia. Here we analyzed the function of DOT1L in breast cancer cells. The expression of DOT1L was up-regulated in malignant breast cancer tissues. Over-expression of DOT1L significantly increased the sphere formation and the cell migration activities of MCF7 breast cancer cell line. In contrast, knockdown of DOT1L reduced the cell migration activity of MDA-MB-231 breast cancer cell line. BCAT1, which encodes a branched-chain amino acid transaminase, was identified as one of the target genes controlled by DOT1L through the regulation of H3K79 methylation. Mechanistic investigation revealed that BCAT1 might be an important regulator responsible for DOT1L-mediated sphere formation and cell migration in breast cancer cells.
•DOT1L histone methyltransferase is up-regulated in malignant breast cancers.•DOT1L is involved in sphere formation and cell migration of breast cancer cell lines.•DOT1L regulates the expression of BCAT1 through histone H3K79 methylation.•BCAT1 is responsible for DOT1L-mediated cell migration and sphere formation.</abstract><cop>France</cop><pub>Elsevier B.V</pub><pmid>26783998</pmid><doi>10.1016/j.biochi.2016.01.005</doi><tpages>12</tpages></addata></record> |
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| ispartof | Biochimie, 2016-04, Vol.123, p.20-31 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Breast Neoplasms - metabolism Breast Neoplasms - pathology Cancer progression Cell Line, Tumor Cell migration Female Gene Expression Regulation Histone methyltransferase Humans Methylation Methyltransferases - metabolism Neoplasm Metastasis Sphere formation Transaminases - genetics Transcriptional regulation |
| title | DOT1L histone methyltransferase regulates the expression of BCAT1 and is involved in sphere formation and cell migration of breast cancer cell lines |
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