Combined Effects of UV-B, Nitrate, and Low pH Reduce the Survival and Activity Level of Larval Cascades Frogs (Rana cascadae)
We investigated interactions between low pH, high nitrate level, and ultraviolet-B (UV-B) light on the survival and activity level of larval Cascades frogs (Rana cascadae). We used a fully factorial experimental design, with pH levels of 5 and 7; initial “pulse” nitrate exposure levels of 0, 5, and...
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| Veröffentlicht in: | Archives of environmental contamination and toxicology 2000-11, Vol.39 (4), p.494-499 |
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| description | We investigated interactions between low pH, high nitrate level, and ultraviolet-B (UV-B) light on the survival and activity level of larval Cascades frogs (Rana cascadae). We used a fully factorial experimental design, with pH levels of 5 and 7; initial “pulse” nitrate exposure levels of 0, 5, and 20 mg/L; and UV-B present or absent. After a 3-week laboratory exposure, we measured survival and activity level of the larvae. The experiment was repeated two times, in two separate years. Similar effects on survival and activity level were observed in both experiments. R. cascadae survival was not significantly reduced in treatments with individual factors alone (i.e., UV-B control, pH 5 control, or high nitrate level without pH or UV-B). However, in experiments from both years, survival and activity levels of larval R. cascadae were significantly reduced in the treatment with low pH, high nitrate, and UV-B together. In both years, analysis of variance (ANOVA) indicated that pH and nitrate had the greatest effect on survival and that UV-B and nitrate had the greatest effect on activity level. Additional effects were observed in the 1998 experiment on both survival and activity level. In 1998, UV radiation and the interaction term between pH and nitrate (pH × nitrate) had a significant effect on survival. Also in the 1998 experiment, activity level was significantly reduced in treatments at neutral pH with UV, at initial nitrate doses of 5 and 20 mg/L and at neutral pH without UV at an initial nitrate dose of 20 mg/L. We suggest that the adverse effects were due to the multiple stressors acting together. |
| doi_str_mv | 10.1007/s002440010132 |
| format | Article |
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C ; Blaustein, A. R</creator><creatorcontrib>Hatch, A. C ; Blaustein, A. R</creatorcontrib><description>We investigated interactions between low pH, high nitrate level, and ultraviolet-B (UV-B) light on the survival and activity level of larval Cascades frogs (Rana cascadae). We used a fully factorial experimental design, with pH levels of 5 and 7; initial “pulse” nitrate exposure levels of 0, 5, and 20 mg/L; and UV-B present or absent. After a 3-week laboratory exposure, we measured survival and activity level of the larvae. The experiment was repeated two times, in two separate years. Similar effects on survival and activity level were observed in both experiments. R. cascadae survival was not significantly reduced in treatments with individual factors alone (i.e., UV-B control, pH 5 control, or high nitrate level without pH or UV-B). However, in experiments from both years, survival and activity levels of larval R. cascadae were significantly reduced in the treatment with low pH, high nitrate, and UV-B together. In both years, analysis of variance (ANOVA) indicated that pH and nitrate had the greatest effect on survival and that UV-B and nitrate had the greatest effect on activity level. Additional effects were observed in the 1998 experiment on both survival and activity level. In 1998, UV radiation and the interaction term between pH and nitrate (pH × nitrate) had a significant effect on survival. Also in the 1998 experiment, activity level was significantly reduced in treatments at neutral pH with UV, at initial nitrate doses of 5 and 20 mg/L and at neutral pH without UV at an initial nitrate dose of 20 mg/L. We suggest that the adverse effects were due to the multiple stressors acting together.</description><identifier>ISSN: 0090-4341</identifier><identifier>EISSN: 1432-0703</identifier><identifier>DOI: 10.1007/s002440010132</identifier><identifier>PMID: 11031310</identifier><identifier>CODEN: AECTCV</identifier><language>eng</language><publisher>Heidelberg: Springer-Verlag</publisher><subject>adverse effects ; Amphibians ; analysis of variance ; Animal, plant and microbial ecology ; Animals ; Applied ecology ; Biological and medical sciences ; Ecotoxicology, biological effects of pollution ; Effects of pollution and side effects of pesticides on vertebrates ; Experimental design ; Experiments ; Freshwater ; frogs ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Larva - drug effects ; Larva - radiation effects ; Larvae ; Nitrates ; Nitrates - toxicity ; Rana ; Rana cascadae ; Ranidae ; Reptilia. 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C</creatorcontrib><creatorcontrib>Blaustein, A. R</creatorcontrib><title>Combined Effects of UV-B, Nitrate, and Low pH Reduce the Survival and Activity Level of Larval Cascades Frogs (Rana cascadae)</title><title>Archives of environmental contamination and toxicology</title><addtitle>Arch Environ Contam Toxicol</addtitle><description>We investigated interactions between low pH, high nitrate level, and ultraviolet-B (UV-B) light on the survival and activity level of larval Cascades frogs (Rana cascadae). We used a fully factorial experimental design, with pH levels of 5 and 7; initial “pulse” nitrate exposure levels of 0, 5, and 20 mg/L; and UV-B present or absent. After a 3-week laboratory exposure, we measured survival and activity level of the larvae. The experiment was repeated two times, in two separate years. Similar effects on survival and activity level were observed in both experiments. R. cascadae survival was not significantly reduced in treatments with individual factors alone (i.e., UV-B control, pH 5 control, or high nitrate level without pH or UV-B). However, in experiments from both years, survival and activity levels of larval R. cascadae were significantly reduced in the treatment with low pH, high nitrate, and UV-B together. In both years, analysis of variance (ANOVA) indicated that pH and nitrate had the greatest effect on survival and that UV-B and nitrate had the greatest effect on activity level. Additional effects were observed in the 1998 experiment on both survival and activity level. In 1998, UV radiation and the interaction term between pH and nitrate (pH × nitrate) had a significant effect on survival. Also in the 1998 experiment, activity level was significantly reduced in treatments at neutral pH with UV, at initial nitrate doses of 5 and 20 mg/L and at neutral pH without UV at an initial nitrate dose of 20 mg/L. We suggest that the adverse effects were due to the multiple stressors acting together.</description><subject>adverse effects</subject><subject>Amphibians</subject><subject>analysis of variance</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effects of pollution and side effects of pesticides on vertebrates</subject><subject>Experimental design</subject><subject>Experiments</subject><subject>Freshwater</subject><subject>frogs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Larva - drug effects</subject><subject>Larva - radiation effects</subject><subject>Larvae</subject><subject>Nitrates</subject><subject>Nitrates - toxicity</subject><subject>Rana</subject><subject>Rana cascadae</subject><subject>Ranidae</subject><subject>Reptilia. Amphibia</subject><subject>Survival</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><subject>Variance analysis</subject><subject>Water Pollutants - analysis</subject><issn>0090-4341</issn><issn>1432-0703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0ctv1DAQB2ALgehSOHIFS0gIpAbGrzyOZdVSpBVILcs1mrXHJVU22drJVj30f8fprnhderJkf_qNZ4axlwI-CIDiYwSQWgMIEEo-YjOhlcygAPWYzQAqyLTS4oA9i_EqIVmW-ik7EAKUUAJm7G7er1dNR46feE92iLz3fPkj-3TEvzZDwIGOOHaOL_obvjnj5-RGS3z4SfxiDNtmi-3987Edmm0z3PIFbamdMhYYpsc5RouOIj8N_WXk786xQ27vL5HeP2dPPLaRXuzPQ7Y8Pfk-P8sW3z5_mR8vMqtzOWTCI6AvKC8x_RGkKpwzRIoQHDgjpa1QA9rUqVp5hZWxVDgDBIXPtVfqkL3d5W5Cfz1SHOp1Ey21LXbUj7EWRaHylPMwzEUFOhcPQ22MhCJP8M1_8KofQ5e6rYURhTY6rS6pbKds6GMM5OtNaNYYbmsB9bTn-p89J_9qnzqu1uT-6P1i_yo7zbr1ATvbxN-ulBWYaS6vd8pjX-NlSGJ5IUGkOmBEWVXqFzyhtUY</recordid><startdate>20001101</startdate><enddate>20001101</enddate><creator>Hatch, A. 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After a 3-week laboratory exposure, we measured survival and activity level of the larvae. The experiment was repeated two times, in two separate years. Similar effects on survival and activity level were observed in both experiments. R. cascadae survival was not significantly reduced in treatments with individual factors alone (i.e., UV-B control, pH 5 control, or high nitrate level without pH or UV-B). However, in experiments from both years, survival and activity levels of larval R. cascadae were significantly reduced in the treatment with low pH, high nitrate, and UV-B together. In both years, analysis of variance (ANOVA) indicated that pH and nitrate had the greatest effect on survival and that UV-B and nitrate had the greatest effect on activity level. Additional effects were observed in the 1998 experiment on both survival and activity level. In 1998, UV radiation and the interaction term between pH and nitrate (pH × nitrate) had a significant effect on survival. Also in the 1998 experiment, activity level was significantly reduced in treatments at neutral pH with UV, at initial nitrate doses of 5 and 20 mg/L and at neutral pH without UV at an initial nitrate dose of 20 mg/L. We suggest that the adverse effects were due to the multiple stressors acting together.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><cop>New York, NY</cop><pub>Springer-Verlag</pub><pmid>11031310</pmid><doi>10.1007/s002440010132</doi><tpages>6</tpages></addata></record> |
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| subjects | adverse effects Amphibians analysis of variance Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on vertebrates Experimental design Experiments Freshwater frogs Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Larva - drug effects Larva - radiation effects Larvae Nitrates Nitrates - toxicity Rana Rana cascadae Ranidae Reptilia. Amphibia Survival Ultraviolet radiation Ultraviolet Rays Variance analysis Water Pollutants - analysis |
| title | Combined Effects of UV-B, Nitrate, and Low pH Reduce the Survival and Activity Level of Larval Cascades Frogs (Rana cascadae) |
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