hosoba toge toge, a syndrome caused by a large chromosomal deletion associated with a T-DNA insertion in Arabidopsis

We isolated a T-DNA-tagged mutant named hosoba toge toge (hot) in which a pleiotropic phenotype was observed in both the shoot and root throughout the life cycle. The phenotype and allelism indicated that the mutant has a defect in both the FASCIATA1 (FAS1) gene and the FT gene located on the bottom...

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Veröffentlicht in:	Plant and cell physiology 2000-09, Vol.41 (9), p.1055-1066
Hauptverfasser:	Kaya, H. (Kyoto Univ. (Japan)), Sato, S, Tabata, S, Kobayashi, Y, Iwabuchi, M, Araki, T
Format:	Artikel
Sprache:	eng
Schlagworte:	5-triphosphate 5-phosphatase ACC aminocyclopropane-1-carboxylate aminoglycoside phosphotransferase APH ARABIDOPSIS Arabidopsis - genetics Arabidopsis - growth & development BAC bacterial artificial chromosome CHROMOSOME ABERRATIONS Chromosome Deletion Chromosome Mapping Deoxyribonucleic acid DNA DNA, Bacterial - genetics DNA, Single-Stranded - genetics endo-1,4-^b-glucanase Enzymes - genetics EST expressed sequence tag FAS1 gene FT gene GENE LOCATION GENE TRANSFER Genome, Plant hosoba toge toge syndrome Humans inositol-1 IP5Pase IP5Pase gene Key words: Arabidopsis — Chromosomal deletion — FASCIATA1-FT region —T-DNA insertion left border MS medium Murashige and Skoog’s medium Mutagenesis MUTANTS N-acetyl-β-glucosaminidase NAGase NAGase gene NAPDH oxidoreductase PHENOTYPES Plant Leaves Plant Roots polygalacturonase Polymerase Chain Reaction reverse transcription PCR right border RT-PCR SAM shoot apical meristem TAIL-PCR thermal asymmetric interlaced PCR
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