Altered protein mannosylation in developing cerebral cortex by streptomycin
Our research objective was to characterize the biochemical effect of streptomycin during postnatal rat cerebral cortex development using a sensitive method that preserves the in situ topological relationship. We found a decrease in the mannosylation of asparagine-linked oligosaccharides without affe...
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| Veröffentlicht in: | Neuroreport 2000-11, Vol.11 (16), p.3569-3572 |
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| container_title | Neuroreport |
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| creator | Alperin, Mario D Calandria, Jorgelina M Carminatti, Hector Idoyaga-Vargas, Victor P |
| description | Our research objective was to characterize the biochemical effect of streptomycin during postnatal rat cerebral cortex development using a sensitive method that preserves the in situ topological relationship. We found a decrease in the mannosylation of asparagine-linked oligosaccharides without affecting polypeptide synthesis, DNA synthesis or glucose and mannose disappearance from the medium in mini-tissue units derived from P5. In addition, the rate of Dolp-GlcNAc2Man9Glc3 synthesis and the oligosaccharide protein transferase activity did not change in the presence of the aminoglycoside. These findings strongly suggested that the alteration of protein mannosylation occurred downstream of G3 transfer to nascent polypeptides. Further, the mini-tissue units may be useful for the assessment of neurological toxicity of antibacterial agents. |
| doi_str_mv | 10.1097/00001756-200011090-00033 |
| format | Article |
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We found a decrease in the mannosylation of asparagine-linked oligosaccharides without affecting polypeptide synthesis, DNA synthesis or glucose and mannose disappearance from the medium in mini-tissue units derived from P5. In addition, the rate of Dolp-GlcNAc2Man9Glc3 synthesis and the oligosaccharide protein transferase activity did not change in the presence of the aminoglycoside. These findings strongly suggested that the alteration of protein mannosylation occurred downstream of G3 transfer to nascent polypeptides. Further, the mini-tissue units may be useful for the assessment of neurological toxicity of antibacterial agents.</description><identifier>ISSN: 0959-4965</identifier><identifier>EISSN: 1473-558X</identifier><identifier>DOI: 10.1097/00001756-200011090-00033</identifier><identifier>PMID: 11095520</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins, Inc</publisher><subject>Animals ; Biochemistry and metabolism ; Biological and medical sciences ; Carbohydrate Sequence ; Central nervous system ; Cerebral Cortex - drug effects ; Cerebral Cortex - metabolism ; Fundamental and applied biological sciences. Psychology ; Glucose - metabolism ; Glycosylation - drug effects ; Hexosyltransferases ; Kinetics ; Mannose - metabolism ; mannosylation ; Membrane Proteins ; Molecular Sequence Data ; Oligosaccharides - biosynthesis ; Oligosaccharides - chemistry ; Rats ; Rats, Wistar ; Streptomycin - pharmacology ; Transferases - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroreport, 2000-11, Vol.11 (16), p.3569-3572</ispartof><rights>2000 Lippincott Williams & Wilkins, Inc.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3603-74991ccd14b176ef8c5116d029bff0f6324171b385ae145aad699119b287c8223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=893471$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11095520$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alperin, Mario D</creatorcontrib><creatorcontrib>Calandria, Jorgelina M</creatorcontrib><creatorcontrib>Carminatti, Hector</creatorcontrib><creatorcontrib>Idoyaga-Vargas, Victor P</creatorcontrib><title>Altered protein mannosylation in developing cerebral cortex by streptomycin</title><title>Neuroreport</title><addtitle>Neuroreport</addtitle><description>Our research objective was to characterize the biochemical effect of streptomycin during postnatal rat cerebral cortex development using a sensitive method that preserves the in situ topological relationship. We found a decrease in the mannosylation of asparagine-linked oligosaccharides without affecting polypeptide synthesis, DNA synthesis or glucose and mannose disappearance from the medium in mini-tissue units derived from P5. In addition, the rate of Dolp-GlcNAc2Man9Glc3 synthesis and the oligosaccharide protein transferase activity did not change in the presence of the aminoglycoside. These findings strongly suggested that the alteration of protein mannosylation occurred downstream of G3 transfer to nascent polypeptides. Further, the mini-tissue units may be useful for the assessment of neurological toxicity of antibacterial agents.</description><subject>Animals</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Sequence</subject><subject>Central nervous system</subject><subject>Cerebral Cortex - drug effects</subject><subject>Cerebral Cortex - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>Glycosylation - drug effects</subject><subject>Hexosyltransferases</subject><subject>Kinetics</subject><subject>Mannose - metabolism</subject><subject>mannosylation</subject><subject>Membrane Proteins</subject><subject>Molecular Sequence Data</subject><subject>Oligosaccharides - biosynthesis</subject><subject>Oligosaccharides - chemistry</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Streptomycin - pharmacology</subject><subject>Transferases - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0959-4965</issn><issn>1473-558X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1PxCAQhonRuOvqXzBNTLxVoZRSjhvjV9zEiybeCKVTt0pLha7r_nupW9eTXJhMnpeZPCAUEXxBsOCXOBzCWRYnQxFaOA4FpXtoSlJOY8byl300xYKJOBUZm6Aj798CIjDJD9FkiDCW4Cl6mJseHJRR52wPdRs1qm2t3xjV17aNQqOETzC2q9vXSAeycMpE2roevqJiE_neQdfbZqPr9hgdVMp4OBnvGXq-uX66uosXj7f3V_NFrGmGacxTIYjWJUkLwjOocs0IyUqciKKqcJXRJCWcFDRnCkjKlCqzECCiSHKu8yShM3S-fTfs_LEC38um9hqMUS3YlZeEc5qESADzLaid9d5BJTtXN8ptJMFyECl_RcqdSPkjMkRPxxmrooHyLziaC8DZCCivlamcanXtd1wuaMqHDdIttbaDZ_9uVmtwcgnK9Ev53zfSb4ZJif8</recordid><startdate>20001109</startdate><enddate>20001109</enddate><creator>Alperin, Mario D</creator><creator>Calandria, Jorgelina M</creator><creator>Carminatti, Hector</creator><creator>Idoyaga-Vargas, Victor P</creator><general>Lippincott Williams & Wilkins, Inc</general><general>Lippincott Williams and Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20001109</creationdate><title>Altered protein mannosylation in developing cerebral cortex by streptomycin</title><author>Alperin, Mario D ; Calandria, Jorgelina M ; Carminatti, Hector ; Idoyaga-Vargas, Victor P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3603-74991ccd14b176ef8c5116d029bff0f6324171b385ae145aad699119b287c8223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate Sequence</topic><topic>Central nervous system</topic><topic>Cerebral Cortex - drug effects</topic><topic>Cerebral Cortex - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>Glycosylation - drug effects</topic><topic>Hexosyltransferases</topic><topic>Kinetics</topic><topic>Mannose - metabolism</topic><topic>mannosylation</topic><topic>Membrane Proteins</topic><topic>Molecular Sequence Data</topic><topic>Oligosaccharides - biosynthesis</topic><topic>Oligosaccharides - chemistry</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Streptomycin - pharmacology</topic><topic>Transferases - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alperin, Mario D</creatorcontrib><creatorcontrib>Calandria, Jorgelina M</creatorcontrib><creatorcontrib>Carminatti, Hector</creatorcontrib><creatorcontrib>Idoyaga-Vargas, Victor P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alperin, Mario D</au><au>Calandria, Jorgelina M</au><au>Carminatti, Hector</au><au>Idoyaga-Vargas, Victor P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Altered protein mannosylation in developing cerebral cortex by streptomycin</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>2000-11-09</date><risdate>2000</risdate><volume>11</volume><issue>16</issue><spage>3569</spage><epage>3572</epage><pages>3569-3572</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>Our research objective was to characterize the biochemical effect of streptomycin during postnatal rat cerebral cortex development using a sensitive method that preserves the in situ topological relationship. We found a decrease in the mannosylation of asparagine-linked oligosaccharides without affecting polypeptide synthesis, DNA synthesis or glucose and mannose disappearance from the medium in mini-tissue units derived from P5. In addition, the rate of Dolp-GlcNAc2Man9Glc3 synthesis and the oligosaccharide protein transferase activity did not change in the presence of the aminoglycoside. These findings strongly suggested that the alteration of protein mannosylation occurred downstream of G3 transfer to nascent polypeptides. Further, the mini-tissue units may be useful for the assessment of neurological toxicity of antibacterial agents.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>11095520</pmid><doi>10.1097/00001756-200011090-00033</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Biochemistry and metabolism Biological and medical sciences Carbohydrate Sequence Central nervous system Cerebral Cortex - drug effects Cerebral Cortex - metabolism Fundamental and applied biological sciences. Psychology Glucose - metabolism Glycosylation - drug effects Hexosyltransferases Kinetics Mannose - metabolism mannosylation Membrane Proteins Molecular Sequence Data Oligosaccharides - biosynthesis Oligosaccharides - chemistry Rats Rats, Wistar Streptomycin - pharmacology Transferases - metabolism Vertebrates: nervous system and sense organs |
| title | Altered protein mannosylation in developing cerebral cortex by streptomycin |
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