Nitric oxide activation of TrkB through peroxynitrite
NIH-3T3 cells stably transfected with TrkB, the receptor for brain-derived neurotrophic factor (BDNF), were used to study the effects of NO and peroxynitrite on TrkB. 3-Morpholinosydnonimine (SIN-1), a donor of NO and O2 – which immediately react to form peroxynitrite, induced TrkB tyrosine phosphor...
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| Veröffentlicht in: | Neuroreport 2000-11, Vol.11 (16), p.3593-3597 |
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| creator | Yuen, Eric C Gunther, Erik C Bothwell, Mark |
| description | NIH-3T3 cells stably transfected with TrkB, the receptor for brain-derived neurotrophic factor (BDNF), were used to study the effects of NO and peroxynitrite on TrkB. 3-Morpholinosydnonimine (SIN-1), a donor of NO and O2 – which immediately react to form peroxynitrite, induced TrkB tyrosine phosphorylation in a dose-dependent relationship from 2 to 40 mM. TrkB phosphorylation by SIN-1 was blocked by superoxide dismutase, which converts O2 – to H2O2 and prevents its reaction with NO to form peroxynitrite, and by K252a, an inhibitor of TrkB phosphorylation by BDNF. Treatment with NO or O2 – alone did not activate TrkB. Treatment directly with 1–4 mM peroxynitrite resulted in a dose-dependent increase in tyrosine phosphorylation of TrkB. SIN-1 treatment induced tyrosine phosphorylation of phospholipase C-γ1 (PLC-γ1) and induced its binding with activated TrkB, similar to that seen with BDNF downstream signaling pathways. These studies demonstrate activation of TrkB through peroxynitrite. |
| doi_str_mv | 10.1097/00001756-200011090-00038 |
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TrkB phosphorylation by SIN-1 was blocked by superoxide dismutase, which converts O2 – to H2O2 and prevents its reaction with NO to form peroxynitrite, and by K252a, an inhibitor of TrkB phosphorylation by BDNF. Treatment with NO or O2 – alone did not activate TrkB. Treatment directly with 1–4 mM peroxynitrite resulted in a dose-dependent increase in tyrosine phosphorylation of TrkB. SIN-1 treatment induced tyrosine phosphorylation of phospholipase C-γ1 (PLC-γ1) and induced its binding with activated TrkB, similar to that seen with BDNF downstream signaling pathways. These studies demonstrate activation of TrkB through peroxynitrite.</description><identifier>ISSN: 0959-4965</identifier><identifier>EISSN: 1473-558X</identifier><identifier>DOI: 10.1097/00001756-200011090-00038</identifier><identifier>PMID: 11095525</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins, Inc</publisher><subject>3T3 Cells ; Animals ; Biochemistry and metabolism ; Biological and medical sciences ; Brain-Derived Neurotrophic Factor - pharmacology ; Carbazoles - pharmacology ; Central nervous system ; Fundamental and applied biological sciences. Psychology ; Humans ; Indole Alkaloids ; Isoenzymes - metabolism ; Mice ; Molsidomine - analogs & derivatives ; Molsidomine - pharmacology ; Nitrates - pharmacology ; Nitric Oxide - physiology ; Nitric Oxide Donors - pharmacology ; Oxidants - pharmacology ; Phospholipase C gamma ; Phosphorylation ; Phosphotyrosine - metabolism ; Rats ; Receptor, trkB - drug effects ; Receptor, trkB - physiology ; Recombinant Proteins - drug effects ; Recombinant Proteins - metabolism ; Recombinant Proteins - pharmacology ; Superoxide Dismutase - pharmacology ; Transfection ; Type C Phospholipases - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroreport, 2000-11, Vol.11 (16), p.3593-3597</ispartof><rights>2000 Lippincott Williams & Wilkins, Inc.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4158-ab635c64d40d250e1d9829b1b975c713915cdc3b0a1ab03a74b28e3dff2171373</citedby><cites>FETCH-LOGICAL-c4158-ab635c64d40d250e1d9829b1b975c713915cdc3b0a1ab03a74b28e3dff2171373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=893309$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11095525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuen, Eric C</creatorcontrib><creatorcontrib>Gunther, Erik C</creatorcontrib><creatorcontrib>Bothwell, Mark</creatorcontrib><title>Nitric oxide activation of TrkB through peroxynitrite</title><title>Neuroreport</title><addtitle>Neuroreport</addtitle><description>NIH-3T3 cells stably transfected with TrkB, the receptor for brain-derived neurotrophic factor (BDNF), were used to study the effects of NO and peroxynitrite on TrkB. 3-Morpholinosydnonimine (SIN-1), a donor of NO and O2 – which immediately react to form peroxynitrite, induced TrkB tyrosine phosphorylation in a dose-dependent relationship from 2 to 40 mM. TrkB phosphorylation by SIN-1 was blocked by superoxide dismutase, which converts O2 – to H2O2 and prevents its reaction with NO to form peroxynitrite, and by K252a, an inhibitor of TrkB phosphorylation by BDNF. Treatment with NO or O2 – alone did not activate TrkB. Treatment directly with 1–4 mM peroxynitrite resulted in a dose-dependent increase in tyrosine phosphorylation of TrkB. SIN-1 treatment induced tyrosine phosphorylation of phospholipase C-γ1 (PLC-γ1) and induced its binding with activated TrkB, similar to that seen with BDNF downstream signaling pathways. These studies demonstrate activation of TrkB through peroxynitrite.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain-Derived Neurotrophic Factor - pharmacology</subject><subject>Carbazoles - pharmacology</subject><subject>Central nervous system</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Indole Alkaloids</subject><subject>Isoenzymes - metabolism</subject><subject>Mice</subject><subject>Molsidomine - analogs & derivatives</subject><subject>Molsidomine - pharmacology</subject><subject>Nitrates - pharmacology</subject><subject>Nitric Oxide - physiology</subject><subject>Nitric Oxide Donors - pharmacology</subject><subject>Oxidants - pharmacology</subject><subject>Phospholipase C gamma</subject><subject>Phosphorylation</subject><subject>Phosphotyrosine - metabolism</subject><subject>Rats</subject><subject>Receptor, trkB - drug effects</subject><subject>Receptor, trkB - physiology</subject><subject>Recombinant Proteins - drug effects</subject><subject>Recombinant Proteins - metabolism</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Superoxide Dismutase - pharmacology</subject><subject>Transfection</subject><subject>Type C Phospholipases - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0959-4965</issn><issn>1473-558X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kV1PwyAUhonRuDn9C6aJiXdVKKXApS5-JYvezMQ7Qim1uK5MaN3276Wuziu5OSec5z0kDwBECF4hyOk1DAdRksVJ34QrGIcGswMwRinFMSHs7RCMISc8TnlGRuDE-4-AcIjYMRj1EUISMgbk2bTOqMhuTKEjqVrzJVtjm8iW0dwtbqO2crZ7r6KVdnazbXq61afgqJS112dDnYDX-7v59DGevTw8TW9msUoRYbHMM0xUlhYpLBICNSo4S3iOck6JoghzRFShcA4lkjnEkqZ5wjQuyjJBYUzxBFzu9q6c_ey0b8XSeKXrWjbadl4gSnFCKQkg24HKWe-dLsXKmaV0W4Gg6JWJX2Vir0z8KAvR8-GNLl_q4i84OArAxQBIr2RdOtko4_cc4xhDHqh0R61t3WrnF3W31k5UWtZtJf77MPwNE2aBqg</recordid><startdate>20001109</startdate><enddate>20001109</enddate><creator>Yuen, Eric C</creator><creator>Gunther, Erik C</creator><creator>Bothwell, Mark</creator><general>Lippincott Williams & Wilkins, Inc</general><general>Lippincott Williams and Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20001109</creationdate><title>Nitric oxide activation of TrkB through peroxynitrite</title><author>Yuen, Eric C ; Gunther, Erik C ; Bothwell, Mark</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4158-ab635c64d40d250e1d9829b1b975c713915cdc3b0a1ab03a74b28e3dff2171373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain-Derived Neurotrophic Factor - pharmacology</topic><topic>Carbazoles - pharmacology</topic><topic>Central nervous system</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Indole Alkaloids</topic><topic>Isoenzymes - metabolism</topic><topic>Mice</topic><topic>Molsidomine - analogs & derivatives</topic><topic>Molsidomine - pharmacology</topic><topic>Nitrates - pharmacology</topic><topic>Nitric Oxide - physiology</topic><topic>Nitric Oxide Donors - pharmacology</topic><topic>Oxidants - pharmacology</topic><topic>Phospholipase C gamma</topic><topic>Phosphorylation</topic><topic>Phosphotyrosine - metabolism</topic><topic>Rats</topic><topic>Receptor, trkB - drug effects</topic><topic>Receptor, trkB - physiology</topic><topic>Recombinant Proteins - drug effects</topic><topic>Recombinant Proteins - metabolism</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Superoxide Dismutase - pharmacology</topic><topic>Transfection</topic><topic>Type C Phospholipases - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuen, Eric C</creatorcontrib><creatorcontrib>Gunther, Erik C</creatorcontrib><creatorcontrib>Bothwell, Mark</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuen, Eric C</au><au>Gunther, Erik C</au><au>Bothwell, Mark</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric oxide activation of TrkB through peroxynitrite</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>2000-11-09</date><risdate>2000</risdate><volume>11</volume><issue>16</issue><spage>3593</spage><epage>3597</epage><pages>3593-3597</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>NIH-3T3 cells stably transfected with TrkB, the receptor for brain-derived neurotrophic factor (BDNF), were used to study the effects of NO and peroxynitrite on TrkB. 3-Morpholinosydnonimine (SIN-1), a donor of NO and O2 – which immediately react to form peroxynitrite, induced TrkB tyrosine phosphorylation in a dose-dependent relationship from 2 to 40 mM. TrkB phosphorylation by SIN-1 was blocked by superoxide dismutase, which converts O2 – to H2O2 and prevents its reaction with NO to form peroxynitrite, and by K252a, an inhibitor of TrkB phosphorylation by BDNF. Treatment with NO or O2 – alone did not activate TrkB. Treatment directly with 1–4 mM peroxynitrite resulted in a dose-dependent increase in tyrosine phosphorylation of TrkB. SIN-1 treatment induced tyrosine phosphorylation of phospholipase C-γ1 (PLC-γ1) and induced its binding with activated TrkB, similar to that seen with BDNF downstream signaling pathways. These studies demonstrate activation of TrkB through peroxynitrite.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>11095525</pmid><doi>10.1097/00001756-200011090-00038</doi><tpages>5</tpages></addata></record> |
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| subjects | 3T3 Cells Animals Biochemistry and metabolism Biological and medical sciences Brain-Derived Neurotrophic Factor - pharmacology Carbazoles - pharmacology Central nervous system Fundamental and applied biological sciences. Psychology Humans Indole Alkaloids Isoenzymes - metabolism Mice Molsidomine - analogs & derivatives Molsidomine - pharmacology Nitrates - pharmacology Nitric Oxide - physiology Nitric Oxide Donors - pharmacology Oxidants - pharmacology Phospholipase C gamma Phosphorylation Phosphotyrosine - metabolism Rats Receptor, trkB - drug effects Receptor, trkB - physiology Recombinant Proteins - drug effects Recombinant Proteins - metabolism Recombinant Proteins - pharmacology Superoxide Dismutase - pharmacology Transfection Type C Phospholipases - metabolism Vertebrates: nervous system and sense organs |
| title | Nitric oxide activation of TrkB through peroxynitrite |
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