Locked Nucleic Acid: A Potent Nucleic Acid Analog in Therapeutics and Biotechnology
Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing very high affinity and excellent specificity toward complementary DNA and RNA, and LNA oligonucleotides have been applied as antisense molecules both in vitro and in vivo . In this review, we briefly describe the basic physioche...
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| Veröffentlicht in: | Oligonucleotides 2004-01, Vol.14 (2), p.13-146 |
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| creator | Jepsen, Jan Stenvang Sørensen, Mads D. Wengel, Jesper |
| description | Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing very high affinity and excellent specificity toward complementary DNA and RNA, and LNA oligonucleotides have been applied as antisense molecules both
in vitro
and
in vivo
. In this review, we briefly
describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects,
toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping. |
| doi_str_mv | 10.1089/1545457041526317 |
| format | Article |
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in vitro
and
in vivo
. In this review, we briefly
describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects,
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in vitro
and
in vivo
. In this review, we briefly
describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects,
toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping.</description><subject>Acids</subject><subject>Animals</subject><subject>Biotechnology</subject><subject>Biotechnology - methods</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Drug Design</subject><subject>Gene expression</subject><subject>Genetics</subject><subject>Humans</subject><subject>Nucleic acids</subject><subject>Oligonucleotides</subject><subject>Oligonucleotides, Antisense - genetics</subject><subject>Oligonucleotides, Antisense - metabolism</subject><subject>Oligonucleotides, Antisense - pharmacology</subject><subject>Oligonucleotides, Antisense - therapeutic use</subject><subject>Pharmacokinetics</subject><subject>Reviews</subject><subject>Ribonuclease H - metabolism</subject><issn>1545-4576</issn><issn>2159-3337</issn><issn>1557-8526</issn><issn>2159-3345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkL1PwzAQxS0EoqWwMyGLgS3gj9hO2ErFl1QBEmWOHNuhLqld7GTof4-rVkJ0QR7Od_e7J70HwDlG1xgV5Q1meXoC5ZgRTrE4AEPMmMiK1B5u_jnL0p4PwEmMC4QIK5A4BoOElzkSfAjep159GQ1fetUaq-BYWX0Lx_DNd8Z1f8Zw7GTrP6F1cDY3Qa5M31kVoXQa3tnEq7nzCVifgqNGttGc7eoIfDzczyZP2fT18XkynmYq57zLGGVcNqzBijJWYoYK3tRY5zXlqC5JMlRzhEzJKdOaJIsl0qLBtaKkSMYQHYGrre4q-O_exK5a2qhM20pnfB8rLATFhJEEXu6BC9-H5CZWBHNCKC5ogtAWUsHHGExTrYJdyrCuMKo2aVf7aaeTi51uXy-N_j3YxZsAvgU2Y-lca01tQve_8g-RA4dH</recordid><startdate>20040101</startdate><enddate>20040101</enddate><creator>Jepsen, Jan Stenvang</creator><creator>Sørensen, Mads D.</creator><creator>Wengel, Jesper</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7QO</scope></search><sort><creationdate>20040101</creationdate><title>Locked Nucleic Acid: A Potent Nucleic Acid Analog in Therapeutics and Biotechnology</title><author>Jepsen, Jan Stenvang ; 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in vitro
and
in vivo
. In this review, we briefly
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toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>15294076</pmid><doi>10.1089/1545457041526317</doi><tpages>134</tpages></addata></record> |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Acids Animals Biotechnology Biotechnology - methods Deoxyribonucleic acid DNA Drug Design Gene expression Genetics Humans Nucleic acids Oligonucleotides Oligonucleotides, Antisense - genetics Oligonucleotides, Antisense - metabolism Oligonucleotides, Antisense - pharmacology Oligonucleotides, Antisense - therapeutic use Pharmacokinetics Reviews Ribonuclease H - metabolism |
| title | Locked Nucleic Acid: A Potent Nucleic Acid Analog in Therapeutics and Biotechnology |
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