Regulation of Anti‐Müllerian Hormone and Its Receptor Expression around Follicle Deviation in Cattle
The anti‐Müllerian hormone (AMH) is an important marker of ovarian reserve and for predicting the response to superovulatory treatments in several species. The objective of this study was to investigate whether AMH and its receptor (AMHR2) are regulated in bovine granulosa cells during follicular de...
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| Veröffentlicht in: | Reproduction in domestic animals 2016-04, Vol.51 (2), p.188-194 |
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| container_title | Reproduction in domestic animals |
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| creator | Ilha, GF Rovani, MT Gasperin, BG Ferreira, R de Macedo, MP Neto, OA Duggavathi, R Bordignon, V Gonçalves, PBD |
| description | The anti‐Müllerian hormone (AMH) is an important marker of ovarian reserve and for predicting the response to superovulatory treatments in several species. The objective of this study was to investigate whether AMH and its receptor (AMHR2) are regulated in bovine granulosa cells during follicular development. In the first experiment, granulosa cells were retrieved from the two largest follicles on days 2 (before), 3 (at the expected time) or 4 (after deviation) of follicular wave. In the second experiment, four doses of FSH (30, 30, 20 and 20 mg) or saline were administered twice a day starting on Day 2 of the first follicular wave of the cycle. Granulosa cells and follicular fluid were collected from the two largest follicles 12 h after the last injection of FSH or saline. AMH mRNA abundance was similar in granulosa cells of the two largest follicles (F1 and F2) before deviation (Day 2), but greater in dominant (DF) than subordinate follicles (SF) at the expected time (Day 3) and after (Day 4) deviation (p 0.05), but they tended to be greater in DFs than SFs (p 0.05) between both co‐dominant follicles collected from the FSH‐treated cows. These findings indicate the followings: AMH mRNA levels decrease in both DFs and SFs during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR2 mRNA expression in granulosa cells of co‐dominant follicles. |
| doi_str_mv | 10.1111/rda.12662 |
| format | Article |
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The objective of this study was to investigate whether AMH and its receptor (AMHR2) are regulated in bovine granulosa cells during follicular development. In the first experiment, granulosa cells were retrieved from the two largest follicles on days 2 (before), 3 (at the expected time) or 4 (after deviation) of follicular wave. In the second experiment, four doses of FSH (30, 30, 20 and 20 mg) or saline were administered twice a day starting on Day 2 of the first follicular wave of the cycle. Granulosa cells and follicular fluid were collected from the two largest follicles 12 h after the last injection of FSH or saline. AMH mRNA abundance was similar in granulosa cells of the two largest follicles (F1 and F2) before deviation (Day 2), but greater in dominant (DF) than subordinate follicles (SF) at the expected time (Day 3) and after (Day 4) deviation (p < 0.05). In experiment 1, AMH mRNA levels declined in both DF and SF near the expected time and after deviation when compared to before deviation. There was no difference in AMHR2 mRNA levels before and during follicular deviation (p > 0.05), but they tended to be greater in DFs than SFs (p < 0.1) after deviation. Experiment 2 showed that AMH and AMHR2 mRNA in granulosa cells and AMH protein abundance in follicular fluid were similar (p > 0.05) between both co‐dominant follicles collected from the FSH‐treated cows. These findings indicate the followings: AMH mRNA levels decrease in both DFs and SFs during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR2 mRNA expression in granulosa cells of co‐dominant follicles.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12662</identifier><identifier>PMID: 26815645</identifier><language>eng</language><publisher>Germany: P. Parey Scientific Publishers</publisher><subject>Animal reproduction ; Animals ; Anti-Mullerian Hormone - genetics ; Anti-Mullerian Hormone - metabolism ; Cattle ; Cattle - physiology ; Female ; Follicle Stimulating Hormone - pharmacology ; Follicular Atresia - genetics ; Follicular Atresia - physiology ; Follicular Fluid - chemistry ; Follicular Fluid - drug effects ; Gene Expression Regulation - drug effects ; Granulosa Cells - drug effects ; Granulosa Cells - metabolism ; Hormones ; Ovarian Follicle - physiology ; Receptors, Peptide - genetics ; Receptors, Peptide - metabolism ; Receptors, Transforming Growth Factor beta - genetics ; Receptors, Transforming Growth Factor beta - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism</subject><ispartof>Reproduction in domestic animals, 2016-04, Vol.51 (2), p.188-194</ispartof><rights>2016 Blackwell Verlag GmbH</rights><rights>2016 Blackwell Verlag GmbH.</rights><rights>Copyright © 2016 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4812-58baf9b093c301e31ec9552343163834317108d7354337553cfe8f025ba3d5853</citedby><cites>FETCH-LOGICAL-c4812-58baf9b093c301e31ec9552343163834317108d7354337553cfe8f025ba3d5853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.12662$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.12662$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26815645$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ilha, GF</creatorcontrib><creatorcontrib>Rovani, MT</creatorcontrib><creatorcontrib>Gasperin, BG</creatorcontrib><creatorcontrib>Ferreira, R</creatorcontrib><creatorcontrib>de Macedo, MP</creatorcontrib><creatorcontrib>Neto, OA</creatorcontrib><creatorcontrib>Duggavathi, R</creatorcontrib><creatorcontrib>Bordignon, V</creatorcontrib><creatorcontrib>Gonçalves, PBD</creatorcontrib><title>Regulation of Anti‐Müllerian Hormone and Its Receptor Expression around Follicle Deviation in Cattle</title><title>Reproduction in domestic animals</title><addtitle>Reprod Dom Anim</addtitle><description>The anti‐Müllerian hormone (AMH) is an important marker of ovarian reserve and for predicting the response to superovulatory treatments in several species. The objective of this study was to investigate whether AMH and its receptor (AMHR2) are regulated in bovine granulosa cells during follicular development. In the first experiment, granulosa cells were retrieved from the two largest follicles on days 2 (before), 3 (at the expected time) or 4 (after deviation) of follicular wave. In the second experiment, four doses of FSH (30, 30, 20 and 20 mg) or saline were administered twice a day starting on Day 2 of the first follicular wave of the cycle. Granulosa cells and follicular fluid were collected from the two largest follicles 12 h after the last injection of FSH or saline. AMH mRNA abundance was similar in granulosa cells of the two largest follicles (F1 and F2) before deviation (Day 2), but greater in dominant (DF) than subordinate follicles (SF) at the expected time (Day 3) and after (Day 4) deviation (p < 0.05). In experiment 1, AMH mRNA levels declined in both DF and SF near the expected time and after deviation when compared to before deviation. There was no difference in AMHR2 mRNA levels before and during follicular deviation (p > 0.05), but they tended to be greater in DFs than SFs (p < 0.1) after deviation. Experiment 2 showed that AMH and AMHR2 mRNA in granulosa cells and AMH protein abundance in follicular fluid were similar (p > 0.05) between both co‐dominant follicles collected from the FSH‐treated cows. These findings indicate the followings: AMH mRNA levels decrease in both DFs and SFs during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR2 mRNA expression in granulosa cells of co‐dominant follicles.</description><subject>Animal reproduction</subject><subject>Animals</subject><subject>Anti-Mullerian Hormone - genetics</subject><subject>Anti-Mullerian Hormone - metabolism</subject><subject>Cattle</subject><subject>Cattle - physiology</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>Follicular Atresia - genetics</subject><subject>Follicular Atresia - physiology</subject><subject>Follicular Fluid - chemistry</subject><subject>Follicular Fluid - drug effects</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Granulosa Cells - drug effects</subject><subject>Granulosa Cells - metabolism</subject><subject>Hormones</subject><subject>Ovarian Follicle - physiology</subject><subject>Receptors, Peptide - genetics</subject><subject>Receptors, Peptide - metabolism</subject><subject>Receptors, Transforming Growth Factor beta - genetics</subject><subject>Receptors, Transforming Growth Factor beta - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1uEzEUhS0EoiGw4AXAEhtYTOuf8U-WIW3TSgVEoIKd5czciVyccbBnoN31EfpA7PomfRKcTtsFEndzF_c7R0fnIvSSkl2aZy_WdpcyKdkjNKIlnxREcPoYjciEy0IqqXfQs5TOCKFCK_UU7TCpqZClGKHVAla9t50LLQ4Nnradu7m8-nD9x3uIzrb4KMR1aAHbtsbHXcILqGDThYgPzjcRUtoKbQx9Ph8G713lAe_DLzdYuhbPbNd5eI6eNNYneHG3x-j08ODr7Kg4-TQ_nk1PiqrUlBVCL20zWebgFScUOIVqIgTjJaeS6-1SlOhacVFyroTgVQO6IUwsLa-FFnyM3g6-mxh-9pA6s3apAu9tC6FPhirFNJcqy8fozT_oWehjm9PdUiVhJaOZejdQVQwpRWjMJrq1jReGErNt3-T2zW37mX1159gv11A_kPd1Z2BvAH47Dxf_dzKL_em9ZTEoXOrg_EFh4w8jVW7AfPs4N--FZOr7_LPZxn098I0Nxq6iS-b0CyNU5udLzUnJ_wJ2iqXI</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Ilha, GF</creator><creator>Rovani, MT</creator><creator>Gasperin, BG</creator><creator>Ferreira, R</creator><creator>de Macedo, MP</creator><creator>Neto, OA</creator><creator>Duggavathi, R</creator><creator>Bordignon, V</creator><creator>Gonçalves, PBD</creator><general>P. Parey Scientific Publishers</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201604</creationdate><title>Regulation of Anti‐Müllerian Hormone and Its Receptor Expression around Follicle Deviation in Cattle</title><author>Ilha, GF ; Rovani, MT ; Gasperin, BG ; Ferreira, R ; de Macedo, MP ; Neto, OA ; Duggavathi, R ; Bordignon, V ; Gonçalves, PBD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4812-58baf9b093c301e31ec9552343163834317108d7354337553cfe8f025ba3d5853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animal reproduction</topic><topic>Animals</topic><topic>Anti-Mullerian Hormone - genetics</topic><topic>Anti-Mullerian Hormone - metabolism</topic><topic>Cattle</topic><topic>Cattle - physiology</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>Follicular Atresia - genetics</topic><topic>Follicular Atresia - physiology</topic><topic>Follicular Fluid - chemistry</topic><topic>Follicular Fluid - drug effects</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Granulosa Cells - drug effects</topic><topic>Granulosa Cells - metabolism</topic><topic>Hormones</topic><topic>Ovarian Follicle - physiology</topic><topic>Receptors, Peptide - genetics</topic><topic>Receptors, Peptide - metabolism</topic><topic>Receptors, Transforming Growth Factor beta - genetics</topic><topic>Receptors, Transforming Growth Factor beta - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ilha, GF</creatorcontrib><creatorcontrib>Rovani, MT</creatorcontrib><creatorcontrib>Gasperin, BG</creatorcontrib><creatorcontrib>Ferreira, R</creatorcontrib><creatorcontrib>de Macedo, MP</creatorcontrib><creatorcontrib>Neto, OA</creatorcontrib><creatorcontrib>Duggavathi, R</creatorcontrib><creatorcontrib>Bordignon, V</creatorcontrib><creatorcontrib>Gonçalves, PBD</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ilha, GF</au><au>Rovani, MT</au><au>Gasperin, BG</au><au>Ferreira, R</au><au>de Macedo, MP</au><au>Neto, OA</au><au>Duggavathi, R</au><au>Bordignon, V</au><au>Gonçalves, PBD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of Anti‐Müllerian Hormone and Its Receptor Expression around Follicle Deviation in Cattle</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Dom Anim</addtitle><date>2016-04</date><risdate>2016</risdate><volume>51</volume><issue>2</issue><spage>188</spage><epage>194</epage><pages>188-194</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>The anti‐Müllerian hormone (AMH) is an important marker of ovarian reserve and for predicting the response to superovulatory treatments in several species. The objective of this study was to investigate whether AMH and its receptor (AMHR2) are regulated in bovine granulosa cells during follicular development. In the first experiment, granulosa cells were retrieved from the two largest follicles on days 2 (before), 3 (at the expected time) or 4 (after deviation) of follicular wave. In the second experiment, four doses of FSH (30, 30, 20 and 20 mg) or saline were administered twice a day starting on Day 2 of the first follicular wave of the cycle. Granulosa cells and follicular fluid were collected from the two largest follicles 12 h after the last injection of FSH or saline. AMH mRNA abundance was similar in granulosa cells of the two largest follicles (F1 and F2) before deviation (Day 2), but greater in dominant (DF) than subordinate follicles (SF) at the expected time (Day 3) and after (Day 4) deviation (p < 0.05). In experiment 1, AMH mRNA levels declined in both DF and SF near the expected time and after deviation when compared to before deviation. There was no difference in AMHR2 mRNA levels before and during follicular deviation (p > 0.05), but they tended to be greater in DFs than SFs (p < 0.1) after deviation. Experiment 2 showed that AMH and AMHR2 mRNA in granulosa cells and AMH protein abundance in follicular fluid were similar (p > 0.05) between both co‐dominant follicles collected from the FSH‐treated cows. These findings indicate the followings: AMH mRNA levels decrease in both DFs and SFs during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR2 mRNA expression in granulosa cells of co‐dominant follicles.</abstract><cop>Germany</cop><pub>P. Parey Scientific Publishers</pub><pmid>26815645</pmid><doi>10.1111/rda.12662</doi><tpages>7</tpages></addata></record> |
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| subjects | Animal reproduction Animals Anti-Mullerian Hormone - genetics Anti-Mullerian Hormone - metabolism Cattle Cattle - physiology Female Follicle Stimulating Hormone - pharmacology Follicular Atresia - genetics Follicular Atresia - physiology Follicular Fluid - chemistry Follicular Fluid - drug effects Gene Expression Regulation - drug effects Granulosa Cells - drug effects Granulosa Cells - metabolism Hormones Ovarian Follicle - physiology Receptors, Peptide - genetics Receptors, Peptide - metabolism Receptors, Transforming Growth Factor beta - genetics Receptors, Transforming Growth Factor beta - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | Regulation of Anti‐Müllerian Hormone and Its Receptor Expression around Follicle Deviation in Cattle |
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