Morphological transformation by 8-hydroxy-2'-deoxyguanosine in Syrian hamster embryo (SHE) cells
8-Hydroxy-2'-deoxyguanosine (OH8dG) is one of the most prevalent oxidative DNA modifications found in eukaryotic cells. Previous studies have suggested an association between OH8dG formation and carcinogenesis. However, it is unclear whether OH8dG formation results in the necessary genotoxic ev...
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| Veröffentlicht in: | Toxicological sciences 2000-08, Vol.56 (2), p.303-312 |
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| creator | HAIZHOU ZHANG YONG XU KAMENDULIS, L. M KLAUNIG, J. E |
| description | 8-Hydroxy-2'-deoxyguanosine (OH8dG) is one of the most prevalent oxidative DNA modifications found in eukaryotic cells. Previous studies have suggested an association between OH8dG formation and carcinogenesis. However, it is unclear whether OH8dG formation results in the necessary genotoxic events for cancer development. In the present study, the formation of OH8dG and its ability to transform Syrian hamster embryo (SHE) cells was examined. Methylene blue, a photosensitizer that in the presence of light can generate singlet oxygen by a type II mechanism, was used to produce oxidative DNA damage (predominantly OH8dG) in SHE cells. Photoactivated methylene blue produced a dose-dependent increase in OH8dG as well as a dose-dependent increase in morphological transformation in SHE cells. SHE cells transfected with DNA that contained increasing concentrations of OH8dG displayed a dose-dependent increase in morphological transformation. Treatment with beta-carotene (a singlet oxygen quencher) inhibited both the formation of OH8dG and the induction of morphological transformation in photoactivated methylene blue-treated SHE cells. These results suggest that formation of OH8dG can induce morphological transformation and provide further support for a role of OH8dG formation in the carcinogenesis process. |
| doi_str_mv | 10.1093/toxsci/56.2.303 |
| format | Article |
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SHE cells transfected with DNA that contained increasing concentrations of OH8dG displayed a dose-dependent increase in morphological transformation. Treatment with beta-carotene (a singlet oxygen quencher) inhibited both the formation of OH8dG and the induction of morphological transformation in photoactivated methylene blue-treated SHE cells. 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M</creatorcontrib><creatorcontrib>KLAUNIG, J. E</creatorcontrib><title>Morphological transformation by 8-hydroxy-2'-deoxyguanosine in Syrian hamster embryo (SHE) cells</title><title>Toxicological sciences</title><addtitle>Toxicol Sci</addtitle><description>8-Hydroxy-2'-deoxyguanosine (OH8dG) is one of the most prevalent oxidative DNA modifications found in eukaryotic cells. Previous studies have suggested an association between OH8dG formation and carcinogenesis. However, it is unclear whether OH8dG formation results in the necessary genotoxic events for cancer development. In the present study, the formation of OH8dG and its ability to transform Syrian hamster embryo (SHE) cells was examined. Methylene blue, a photosensitizer that in the presence of light can generate singlet oxygen by a type II mechanism, was used to produce oxidative DNA damage (predominantly OH8dG) in SHE cells. Photoactivated methylene blue produced a dose-dependent increase in OH8dG as well as a dose-dependent increase in morphological transformation in SHE cells. SHE cells transfected with DNA that contained increasing concentrations of OH8dG displayed a dose-dependent increase in morphological transformation. Treatment with beta-carotene (a singlet oxygen quencher) inhibited both the formation of OH8dG and the induction of morphological transformation in photoactivated methylene blue-treated SHE cells. These results suggest that formation of OH8dG can induce morphological transformation and provide further support for a role of OH8dG formation in the carcinogenesis process.</description><subject>8-hydroxy-2'-deoxyguanosine</subject><subject>Animals</subject><subject>beta Carotene - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Cell Transformation, Neoplastic - chemically induced</subject><subject>Cricetinae</subject><subject>Deoxyguanosine - analogs & derivatives</subject><subject>Deoxyguanosine - toxicity</subject><subject>Dose-Response Relationship, Drug</subject><subject>Embryo, Mammalian - drug effects</subject><subject>Embryo, Mammalian - pathology</subject><subject>Foods and miscellaneous</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Medical sciences</subject><subject>Mesocricetus</subject><subject>methylene blue</subject><subject>Methylene Blue - toxicity</subject><subject>Transfection</subject><subject>Tumors</subject><issn>1096-6080</issn><issn>1096-0929</issn><issn>1096-0929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkM1LwzAYh4Mobk7P3iQH8ePQLV_NmqOM6YSJh-k5pm26RdpmJi2s_70ZLSgE3h_Jkx8vDwDXGE0xEnTW2IPPzCzmUzKliJ6AcbjmERJEnA6ZowSNwIX33whhzJE4B6PwEE6SjMHXm3X7nS3t1mSqhI1TtS-sq1RjbA3TDibRrsudPXQRuY9yHcK2VbX1ptbQ1HDTOaNquFOVb7SDukpdZ-HDZrV8hJkuS38JzgpVen01zAn4fF5-LFbR-v3ldfG0jjIqWBMxygo9Z3GWi4KIY9SKkSJFHAuNUoZyThIucs7yOdaEFQFAPM0IppwQldAJuOt7987-tNo3sjL-uIGqtW29xPM5wYzSAM56MHPWe6cLuXemUq6TGMmjVNlLlTGXRAap4cfNUN2mlc7_8b3FANwOgPLBYhEkZsb_cTEVcSj6BXragQU</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>HAIZHOU ZHANG</creator><creator>YONG XU</creator><creator>KAMENDULIS, L. 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E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-434fe745cd9f29fe74ea42fb0619e0b40d62869d64d71e24f74e06bc213622a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>8-hydroxy-2'-deoxyguanosine</topic><topic>Animals</topic><topic>beta Carotene - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Cell Transformation, Neoplastic - chemically induced</topic><topic>Cricetinae</topic><topic>Deoxyguanosine - analogs & derivatives</topic><topic>Deoxyguanosine - toxicity</topic><topic>Dose-Response Relationship, Drug</topic><topic>Embryo, Mammalian - drug effects</topic><topic>Embryo, Mammalian - pathology</topic><topic>Foods and miscellaneous</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Medical sciences</topic><topic>Mesocricetus</topic><topic>methylene blue</topic><topic>Methylene Blue - toxicity</topic><topic>Transfection</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HAIZHOU ZHANG</creatorcontrib><creatorcontrib>YONG XU</creatorcontrib><creatorcontrib>KAMENDULIS, L. 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E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological transformation by 8-hydroxy-2'-deoxyguanosine in Syrian hamster embryo (SHE) cells</atitle><jtitle>Toxicological sciences</jtitle><addtitle>Toxicol Sci</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>56</volume><issue>2</issue><spage>303</spage><epage>312</epage><pages>303-312</pages><issn>1096-6080</issn><issn>1096-0929</issn><eissn>1096-0929</eissn><coden>TOSCF2</coden><abstract>8-Hydroxy-2'-deoxyguanosine (OH8dG) is one of the most prevalent oxidative DNA modifications found in eukaryotic cells. Previous studies have suggested an association between OH8dG formation and carcinogenesis. However, it is unclear whether OH8dG formation results in the necessary genotoxic events for cancer development. In the present study, the formation of OH8dG and its ability to transform Syrian hamster embryo (SHE) cells was examined. Methylene blue, a photosensitizer that in the presence of light can generate singlet oxygen by a type II mechanism, was used to produce oxidative DNA damage (predominantly OH8dG) in SHE cells. Photoactivated methylene blue produced a dose-dependent increase in OH8dG as well as a dose-dependent increase in morphological transformation in SHE cells. SHE cells transfected with DNA that contained increasing concentrations of OH8dG displayed a dose-dependent increase in morphological transformation. Treatment with beta-carotene (a singlet oxygen quencher) inhibited both the formation of OH8dG and the induction of morphological transformation in photoactivated methylene blue-treated SHE cells. 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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 8-hydroxy-2'-deoxyguanosine Animals beta Carotene - pharmacology Biological and medical sciences Carcinogenesis, carcinogens and anticarcinogens Cell Transformation, Neoplastic - chemically induced Cricetinae Deoxyguanosine - analogs & derivatives Deoxyguanosine - toxicity Dose-Response Relationship, Drug Embryo, Mammalian - drug effects Embryo, Mammalian - pathology Foods and miscellaneous Free Radical Scavengers - pharmacology Medical sciences Mesocricetus methylene blue Methylene Blue - toxicity Transfection Tumors |
| title | Morphological transformation by 8-hydroxy-2'-deoxyguanosine in Syrian hamster embryo (SHE) cells |
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