Generation of tolerogenic dendritic cells by treatment with mitomycin C: Inhibition of allogeneic t-cell response is mediated by downregulation of ICAM-1, CD80, and CD86
Deliberately generated tolerogenic dendritic cells (DC) might be a useful tool for induction of donor-specific tolerance in transplantation. In this article, the authors study the effect of mitomycin C (MMC)-treated DC on rat T cells and delineate the mechanism of their conversion into tolerogenic c...
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| Veröffentlicht in: | Transplantation 2004-06, Vol.77 (11), p.1761-1764 |
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| creator | JIGA, Lucian P BAUER, Thomas M CHUANG, Jing-Jing OPELZ, Gerhard TERNESS, Peter |
| description | Deliberately generated tolerogenic dendritic cells (DC) might be a useful tool for induction of donor-specific tolerance in transplantation. In this article, the authors study the effect of mitomycin C (MMC)-treated DC on rat T cells and delineate the mechanism of their conversion into tolerogenic cells.
The influence of MMC treatment on the capacity of DC to activate allogeneic T cells was tested in vitro, and the expression of cell surface molecules was studied by flow cytometry.
MMC-treated DC lose their allostimulatory capacity, and this cannot be attributed to cell death or release of MMC. Interestingly, suppressed T cells cannot be restimulated, indicating that MMC-treated DC induce tolerance. MMC treatment selectively decreases adhesion (intercellular adhesion molecule [ICAM]-1) and co-stimulatory (CD80, CD86) molecules. Functional blocking of these molecules with specific antibodies confers to DC the same T-cell-suppressive properties as treatment with MMC.
MMC treatment converts rat DC into tolerogenic cells. This mechanism is mediated by decrease of ICAM-1, CD80, and CD86. |
| doi_str_mv | 10.1097/01.TP.0000131165.37177.6E |
| format | Article |
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The influence of MMC treatment on the capacity of DC to activate allogeneic T cells was tested in vitro, and the expression of cell surface molecules was studied by flow cytometry.
MMC-treated DC lose their allostimulatory capacity, and this cannot be attributed to cell death or release of MMC. Interestingly, suppressed T cells cannot be restimulated, indicating that MMC-treated DC induce tolerance. MMC treatment selectively decreases adhesion (intercellular adhesion molecule [ICAM]-1) and co-stimulatory (CD80, CD86) molecules. Functional blocking of these molecules with specific antibodies confers to DC the same T-cell-suppressive properties as treatment with MMC.
MMC treatment converts rat DC into tolerogenic cells. This mechanism is mediated by decrease of ICAM-1, CD80, and CD86.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/01.TP.0000131165.37177.6E</identifier><identifier>PMID: 15201679</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Alkylating Agents - pharmacology ; Animals ; Antigens, CD - metabolism ; B7-1 Antigen - metabolism ; B7-2 Antigen ; Biological and medical sciences ; Combined surgery. Multiple transplantations ; Dendritic Cells - drug effects ; Dendritic Cells - immunology ; Down-Regulation ; Immune Tolerance ; Intercellular Adhesion Molecule-1 - metabolism ; Medical sciences ; Membrane Glycoproteins - metabolism ; Mitomycin - pharmacology ; Nucleic Acid Synthesis Inhibitors - pharmacology ; Rats ; Rats, Inbred BN ; Rats, Inbred Lew ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; T-Lymphocytes - immunology</subject><ispartof>Transplantation, 2004-06, Vol.77 (11), p.1761-1764</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-6325389ca870a3a1a3bd5036722d3d71a1b06b58011f283d341d78ba67c506733</citedby><cites>FETCH-LOGICAL-c481t-6325389ca870a3a1a3bd5036722d3d71a1b06b58011f283d341d78ba67c506733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15889937$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15201679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JIGA, Lucian P</creatorcontrib><creatorcontrib>BAUER, Thomas M</creatorcontrib><creatorcontrib>CHUANG, Jing-Jing</creatorcontrib><creatorcontrib>OPELZ, Gerhard</creatorcontrib><creatorcontrib>TERNESS, Peter</creatorcontrib><title>Generation of tolerogenic dendritic cells by treatment with mitomycin C: Inhibition of allogeneic t-cell response is mediated by downregulation of ICAM-1, CD80, and CD86</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Deliberately generated tolerogenic dendritic cells (DC) might be a useful tool for induction of donor-specific tolerance in transplantation. In this article, the authors study the effect of mitomycin C (MMC)-treated DC on rat T cells and delineate the mechanism of their conversion into tolerogenic cells.
The influence of MMC treatment on the capacity of DC to activate allogeneic T cells was tested in vitro, and the expression of cell surface molecules was studied by flow cytometry.
MMC-treated DC lose their allostimulatory capacity, and this cannot be attributed to cell death or release of MMC. Interestingly, suppressed T cells cannot be restimulated, indicating that MMC-treated DC induce tolerance. MMC treatment selectively decreases adhesion (intercellular adhesion molecule [ICAM]-1) and co-stimulatory (CD80, CD86) molecules. Functional blocking of these molecules with specific antibodies confers to DC the same T-cell-suppressive properties as treatment with MMC.
MMC treatment converts rat DC into tolerogenic cells. This mechanism is mediated by decrease of ICAM-1, CD80, and CD86.</description><subject>Alkylating Agents - pharmacology</subject><subject>Animals</subject><subject>Antigens, CD - metabolism</subject><subject>B7-1 Antigen - metabolism</subject><subject>B7-2 Antigen</subject><subject>Biological and medical sciences</subject><subject>Combined surgery. Multiple transplantations</subject><subject>Dendritic Cells - drug effects</subject><subject>Dendritic Cells - immunology</subject><subject>Down-Regulation</subject><subject>Immune Tolerance</subject><subject>Intercellular Adhesion Molecule-1 - metabolism</subject><subject>Medical sciences</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Mitomycin - pharmacology</subject><subject>Nucleic Acid Synthesis Inhibitors - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred BN</subject><subject>Rats, Inbred Lew</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>T-Lymphocytes - immunology</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkcFu1DAQhi0EokvhFZA5wKlJPXFiO9zQdimVWtHDcrYc22mNEnuxvar2kfqWOHShzMVz-P5_xvMj9AFIDaTn5wTq7W1NSgEFYF1NOXBes80LtIKOthUjgrxEK0JaqIBSfoLepPSz8B3l_DU6ga4hwHi_Qo-X1tuosgsehxHnMNkY7qx3GhvrTXS5dNpOU8LDAedoVZ6tz_jB5Xs8uxzmg3Yerz_jK3_vBvfXSE3TYmOLOleLHkebdsEni13CszVOZWsWTxMefLR3--nfElfrLzcVnOH1hSBnWHmzdOwtejWqKdl3x_cU_fi62a6_VdffL4viutKtgFwx2nRU9FoJThRVoOhgOkIZbxpDDQcFA2FDJwjA2AhqaAuGi0ExrjvCOKWn6NOT7y6GX3ubspxdWn6gvA37JMuloW3atoD9E6hjSCnaUe6im1U8SCByyUkSkNtb-ZyT_JOTZJuifX8csh_KMZ6Vx2AK8PEIqKTVNEbltUv_cUL0PeX0N-0Pm1A</recordid><startdate>20040615</startdate><enddate>20040615</enddate><creator>JIGA, Lucian P</creator><creator>BAUER, Thomas M</creator><creator>CHUANG, Jing-Jing</creator><creator>OPELZ, Gerhard</creator><creator>TERNESS, Peter</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20040615</creationdate><title>Generation of tolerogenic dendritic cells by treatment with mitomycin C: Inhibition of allogeneic t-cell response is mediated by downregulation of ICAM-1, CD80, and CD86</title><author>JIGA, Lucian P ; BAUER, Thomas M ; CHUANG, Jing-Jing ; OPELZ, Gerhard ; TERNESS, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-6325389ca870a3a1a3bd5036722d3d71a1b06b58011f283d341d78ba67c506733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alkylating Agents - pharmacology</topic><topic>Animals</topic><topic>Antigens, CD - metabolism</topic><topic>B7-1 Antigen - metabolism</topic><topic>B7-2 Antigen</topic><topic>Biological and medical sciences</topic><topic>Combined surgery. Multiple transplantations</topic><topic>Dendritic Cells - drug effects</topic><topic>Dendritic Cells - immunology</topic><topic>Down-Regulation</topic><topic>Immune Tolerance</topic><topic>Intercellular Adhesion Molecule-1 - metabolism</topic><topic>Medical sciences</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Mitomycin - pharmacology</topic><topic>Nucleic Acid Synthesis Inhibitors - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred BN</topic><topic>Rats, Inbred Lew</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JIGA, Lucian P</creatorcontrib><creatorcontrib>BAUER, Thomas M</creatorcontrib><creatorcontrib>CHUANG, Jing-Jing</creatorcontrib><creatorcontrib>OPELZ, Gerhard</creatorcontrib><creatorcontrib>TERNESS, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JIGA, Lucian P</au><au>BAUER, Thomas M</au><au>CHUANG, Jing-Jing</au><au>OPELZ, Gerhard</au><au>TERNESS, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of tolerogenic dendritic cells by treatment with mitomycin C: Inhibition of allogeneic t-cell response is mediated by downregulation of ICAM-1, CD80, and CD86</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>2004-06-15</date><risdate>2004</risdate><volume>77</volume><issue>11</issue><spage>1761</spage><epage>1764</epage><pages>1761-1764</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>Deliberately generated tolerogenic dendritic cells (DC) might be a useful tool for induction of donor-specific tolerance in transplantation. In this article, the authors study the effect of mitomycin C (MMC)-treated DC on rat T cells and delineate the mechanism of their conversion into tolerogenic cells.
The influence of MMC treatment on the capacity of DC to activate allogeneic T cells was tested in vitro, and the expression of cell surface molecules was studied by flow cytometry.
MMC-treated DC lose their allostimulatory capacity, and this cannot be attributed to cell death or release of MMC. Interestingly, suppressed T cells cannot be restimulated, indicating that MMC-treated DC induce tolerance. MMC treatment selectively decreases adhesion (intercellular adhesion molecule [ICAM]-1) and co-stimulatory (CD80, CD86) molecules. Functional blocking of these molecules with specific antibodies confers to DC the same T-cell-suppressive properties as treatment with MMC.
MMC treatment converts rat DC into tolerogenic cells. This mechanism is mediated by decrease of ICAM-1, CD80, and CD86.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>15201679</pmid><doi>10.1097/01.TP.0000131165.37177.6E</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alkylating Agents - pharmacology Animals Antigens, CD - metabolism B7-1 Antigen - metabolism B7-2 Antigen Biological and medical sciences Combined surgery. Multiple transplantations Dendritic Cells - drug effects Dendritic Cells - immunology Down-Regulation Immune Tolerance Intercellular Adhesion Molecule-1 - metabolism Medical sciences Membrane Glycoproteins - metabolism Mitomycin - pharmacology Nucleic Acid Synthesis Inhibitors - pharmacology Rats Rats, Inbred BN Rats, Inbred Lew Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases T-Lymphocytes - immunology |
| title | Generation of tolerogenic dendritic cells by treatment with mitomycin C: Inhibition of allogeneic t-cell response is mediated by downregulation of ICAM-1, CD80, and CD86 |
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