TRANSFORMATION OF SUGAR BEET CELL SUSPENSION CULTURES

A sugar beet transformation method was developed using particle bombardment of short-term suspension cultures of a breeding line FC607. Highly embryogenic suspension cultures derived from leaf callus were bombarded with the uidA (gusA) reporter gene under the control of either the osmotin or protein...

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Veröffentlicht in:	In vitro cellular & developmental biology. Plant 2003-11, Vol.39 (6), p.573-577
Hauptverfasser:	IVIC, SNEZANA D., SMIGOCKI, ANN C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Beta vulgaris beta-glucuronidase biolistic biolistics Biological and medical sciences Biotechnology Biotechnology/Genetic Transformation/Functional Genomics Breeding Callus cell suspension culture Chemical suspensions Cultured cells embryogenesis Embryos Fundamental and applied biological sciences. Psychology gene expression Genetic engineering Genetic engineering applications Genetic technics genetic transformation Genetics and breeding of economic plants Guard cells in vitro regeneration kinases Methods. Procedures. Technologies neomycin phosphotransferase ii npt II particle bombardment Plant breeding: fundamental aspects and methodology Plant cells Plants Polymerase chain reaction reporter genes shoots sugar beet Sugar beets Transgenic animals and transgenic plants Transgenic plants uidA
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