TRANSFORMATION OF SUGAR BEET CELL SUSPENSION CULTURES
A sugar beet transformation method was developed using particle bombardment of short-term suspension cultures of a breeding line FC607. Highly embryogenic suspension cultures derived from leaf callus were bombarded with the uidA (gusA) reporter gene under the control of either the osmotin or protein...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2003-11, Vol.39 (6), p.573-577 |
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| creator | IVIC, SNEZANA D. SMIGOCKI, ANN C. |
| description | A sugar beet transformation method was developed using particle bombardment of short-term suspension cultures of a breeding line FC607. Highly embryogenic suspension cultures derived from leaf callus were bombarded with the uidA (gusA) reporter gene under the control of either the osmotin or proteinase inhibitor II gene promoter, and the npt II selectable marker gene. Transient uidA expression was visualized as 500–4000 blue units per 200 mg of bombarded cells 2 d after bombardment. Stably-transformed calluses were recovered on both kanamycin and paromomycin media. The greatest number of GUS (+) calluses was obtained when 50 or 100 mg l−1 of kanamycin was applied 2 d after transformation for 3–5 wk, followed by either no selection or reduced levels of the antibiotic. PCR analyses of the GUS (+) callus lines revealed the expected size fragment for uidA and npt II genes. Stable incorporation of the uidA gene into the genome was confirmed by Southern blot analyses. Several transformed embryos were detected by histochemical β-glucuronidase (GUS) staining. |
| doi_str_mv | 10.1079/IVP2003433 |
| format | Article |
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Highly embryogenic suspension cultures derived from leaf callus were bombarded with the uidA (gusA) reporter gene under the control of either the osmotin or proteinase inhibitor II gene promoter, and the npt II selectable marker gene. Transient uidA expression was visualized as 500–4000 blue units per 200 mg of bombarded cells 2 d after bombardment. Stably-transformed calluses were recovered on both kanamycin and paromomycin media. The greatest number of GUS (+) calluses was obtained when 50 or 100 mg l−1 of kanamycin was applied 2 d after transformation for 3–5 wk, followed by either no selection or reduced levels of the antibiotic. PCR analyses of the GUS (+) callus lines revealed the expected size fragment for uidA and npt II genes. Stable incorporation of the uidA gene into the genome was confirmed by Southern blot analyses. Several transformed embryos were detected by histochemical β-glucuronidase (GUS) staining.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1079/IVP2003433</identifier><language>eng</language><publisher>Wallingford: CABI Publishing</publisher><subject>Agronomy. Soil science and plant productions ; Beta vulgaris ; beta-glucuronidase ; biolistic ; biolistics ; Biological and medical sciences ; Biotechnology ; Biotechnology/Genetic Transformation/Functional Genomics ; Breeding ; Callus ; cell suspension culture ; Chemical suspensions ; Cultured cells ; embryogenesis ; Embryos ; Fundamental and applied biological sciences. Psychology ; gene expression ; Genetic engineering ; Genetic engineering applications ; Genetic technics ; genetic transformation ; Genetics and breeding of economic plants ; Guard cells ; in vitro regeneration ; kinases ; Methods. Procedures. Technologies ; neomycin phosphotransferase ii ; npt II ; particle bombardment ; Plant breeding: fundamental aspects and methodology ; Plant cells ; Plants ; Polymerase chain reaction ; reporter genes ; shoots ; sugar beet ; Sugar beets ; Transgenic animals and transgenic plants ; Transgenic plants ; uidA</subject><ispartof>In vitro cellular & developmental biology. Plant, 2003-11, Vol.39 (6), p.573-577</ispartof><rights>Society for In Vitro Biology</rights><rights>Copyright 2003 Society for In Vitro Biology</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Society for In Vitro Biology Nov/Dec 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b395t-fa42f513c803cd50c5969be38ab1c2e702b0482ba18a8326f9480324004a41233</citedby><cites>FETCH-LOGICAL-b395t-fa42f513c803cd50c5969be38ab1c2e702b0482ba18a8326f9480324004a41233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1079/IVP2003433$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4293670$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,26955,27901,27902,52338,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15341029$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>IVIC, SNEZANA D.</creatorcontrib><creatorcontrib>SMIGOCKI, ANN C.</creatorcontrib><title>TRANSFORMATION OF SUGAR BEET CELL SUSPENSION CULTURES</title><title>In vitro cellular & developmental biology. Plant</title><description>A sugar beet transformation method was developed using particle bombardment of short-term suspension cultures of a breeding line FC607. Highly embryogenic suspension cultures derived from leaf callus were bombarded with the uidA (gusA) reporter gene under the control of either the osmotin or proteinase inhibitor II gene promoter, and the npt II selectable marker gene. Transient uidA expression was visualized as 500–4000 blue units per 200 mg of bombarded cells 2 d after bombardment. Stably-transformed calluses were recovered on both kanamycin and paromomycin media. The greatest number of GUS (+) calluses was obtained when 50 or 100 mg l−1 of kanamycin was applied 2 d after transformation for 3–5 wk, followed by either no selection or reduced levels of the antibiotic. PCR analyses of the GUS (+) callus lines revealed the expected size fragment for uidA and npt II genes. Stable incorporation of the uidA gene into the genome was confirmed by Southern blot analyses. Several transformed embryos were detected by histochemical β-glucuronidase (GUS) staining.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Beta vulgaris</subject><subject>beta-glucuronidase</subject><subject>biolistic</subject><subject>biolistics</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Biotechnology/Genetic Transformation/Functional Genomics</subject><subject>Breeding</subject><subject>Callus</subject><subject>cell suspension culture</subject><subject>Chemical suspensions</subject><subject>Cultured cells</subject><subject>embryogenesis</subject><subject>Embryos</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Genetic engineering</subject><subject>Genetic engineering applications</subject><subject>Genetic technics</subject><subject>genetic transformation</subject><subject>Genetics and breeding of economic plants</subject><subject>Guard cells</subject><subject>in vitro regeneration</subject><subject>kinases</subject><subject>Methods. Procedures. Technologies</subject><subject>neomycin phosphotransferase ii</subject><subject>npt II</subject><subject>particle bombardment</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plant cells</subject><subject>Plants</subject><subject>Polymerase chain reaction</subject><subject>reporter genes</subject><subject>shoots</subject><subject>sugar beet</subject><subject>Sugar beets</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><subject>uidA</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE1Lw0AQhoMoWKsXz4JFUESIzn5ls8dY0lqIbcmH17CJibSk2brbHvz3bk2x4MHTzvI8884wjnOJ4BEBF0-TtzkGIJSQI6eHKGcu9nxxbGtg1GWUe6fOmTFLAECAeM9haRxMk9Esfg3SyWw6mI0GSTYO4sFzGKaDYRhF9p_Mw2myo8MsSrM4TM6dk1o2prrYv30nG4Xp8MWNZuPJMIjcggi2cWtJcc0QKX0g5TuDkglPFBXxZYFKXHHABVAfFxL50ifYqwW1JqYAVFKECek7d13uWqvPbWU2-WphyqppZFuprckR5wgzLKx480dcqq1u7W45EpwIX3BkpYdOKrUyRld1vtaLldRfOYJ8d778cD4r3-4TpSllU2vZlgtz6GCEIviZfNV5S7NR-pdTizwOFl93uJYqlx_aRmQJBkQABGE2wRr3nVEslGqr_3b6BrhjheI</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>IVIC, SNEZANA D.</creator><creator>SMIGOCKI, ANN C.</creator><general>CABI Publishing</general><general>Cambridge University Press</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20031101</creationdate><title>TRANSFORMATION OF SUGAR BEET CELL SUSPENSION CULTURES</title><author>IVIC, SNEZANA D. ; SMIGOCKI, ANN C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b395t-fa42f513c803cd50c5969be38ab1c2e702b0482ba18a8326f9480324004a41233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Beta vulgaris</topic><topic>beta-glucuronidase</topic><topic>biolistic</topic><topic>biolistics</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Biotechnology/Genetic Transformation/Functional Genomics</topic><topic>Breeding</topic><topic>Callus</topic><topic>cell suspension culture</topic><topic>Chemical suspensions</topic><topic>Cultured cells</topic><topic>embryogenesis</topic><topic>Embryos</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Genetic engineering</topic><topic>Genetic engineering applications</topic><topic>Genetic technics</topic><topic>genetic transformation</topic><topic>Genetics and breeding of economic plants</topic><topic>Guard cells</topic><topic>in vitro regeneration</topic><topic>kinases</topic><topic>Methods. Procedures. Technologies</topic><topic>neomycin phosphotransferase ii</topic><topic>npt II</topic><topic>particle bombardment</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plant cells</topic><topic>Plants</topic><topic>Polymerase chain reaction</topic><topic>reporter genes</topic><topic>shoots</topic><topic>sugar beet</topic><topic>Sugar beets</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><topic>uidA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>IVIC, SNEZANA D.</creatorcontrib><creatorcontrib>SMIGOCKI, ANN C.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>IVIC, SNEZANA D.</au><au>SMIGOCKI, ANN C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TRANSFORMATION OF SUGAR BEET CELL SUSPENSION CULTURES</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><date>2003-11-01</date><risdate>2003</risdate><volume>39</volume><issue>6</issue><spage>573</spage><epage>577</epage><pages>573-577</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>A sugar beet transformation method was developed using particle bombardment of short-term suspension cultures of a breeding line FC607. Highly embryogenic suspension cultures derived from leaf callus were bombarded with the uidA (gusA) reporter gene under the control of either the osmotin or proteinase inhibitor II gene promoter, and the npt II selectable marker gene. Transient uidA expression was visualized as 500–4000 blue units per 200 mg of bombarded cells 2 d after bombardment. Stably-transformed calluses were recovered on both kanamycin and paromomycin media. The greatest number of GUS (+) calluses was obtained when 50 or 100 mg l−1 of kanamycin was applied 2 d after transformation for 3–5 wk, followed by either no selection or reduced levels of the antibiotic. PCR analyses of the GUS (+) callus lines revealed the expected size fragment for uidA and npt II genes. Stable incorporation of the uidA gene into the genome was confirmed by Southern blot analyses. Several transformed embryos were detected by histochemical β-glucuronidase (GUS) staining.</abstract><cop>Wallingford</cop><pub>CABI Publishing</pub><doi>10.1079/IVP2003433</doi><tpages>5</tpages></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Plant, 2003-11, Vol.39 (6), p.573-577 |
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| subjects | Agronomy. Soil science and plant productions Beta vulgaris beta-glucuronidase biolistic biolistics Biological and medical sciences Biotechnology Biotechnology/Genetic Transformation/Functional Genomics Breeding Callus cell suspension culture Chemical suspensions Cultured cells embryogenesis Embryos Fundamental and applied biological sciences. Psychology gene expression Genetic engineering Genetic engineering applications Genetic technics genetic transformation Genetics and breeding of economic plants Guard cells in vitro regeneration kinases Methods. Procedures. Technologies neomycin phosphotransferase ii npt II particle bombardment Plant breeding: fundamental aspects and methodology Plant cells Plants Polymerase chain reaction reporter genes shoots sugar beet Sugar beets Transgenic animals and transgenic plants Transgenic plants uidA |
| title | TRANSFORMATION OF SUGAR BEET CELL SUSPENSION CULTURES |
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