Angiotensin II type 2 receptor is expressed in human sperm cells and is involved in sperm motility
Objective To investigate the presence of angiotensin II type 2 receptor (AT2R) in human spermatozoa and its implication in sperm fertility status. Design We carried out expression assays for AT2R by reverse transcription–polymerase chain reaction, Western blot, immunofluorescence, and flow cytometry...
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| Veröffentlicht in: | Fertility and sterility 2016-03, Vol.105 (3), p.608-616 |
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| creator | Gianzo, Marta, J.D Muñoa-Hoyos, Iraia, J.D Urizar-Arenaza, Itziar, J.D Larreategui, Zaloa, J.D Quintana, Fernando, J.D Garrido, Nicolás, Ph.D Subirán, Nerea, Ph.D Irazusta, Jon, Ph.D |
| description | Objective To investigate the presence of angiotensin II type 2 receptor (AT2R) in human spermatozoa and its implication in sperm fertility status. Design We carried out expression assays for AT2R by reverse transcription–polymerase chain reaction, Western blot, immunofluorescence, and flow cytometry techniques in human sperm cells. Percentage of AT2R-positive sperm cells was analyzed by flow cytometry. Setting Assisted reproduction unit and academic research laboratory. Patient(s) Ninety-seven human semen samples from the Clínica IVI Bilbao. Intervention(s) All samples were examined and classified according to World Health Organization guidelines. Spermatozoa were isolated from semen on discontinuous colloidal silica gradient (45%–90%) and swim-up techniques. Main Outcome Measure(s) Presence and location of the AT2R in spermatozoa and percentage of AT2R-positive sperm cells measured by flow cytometry. Result(s) We demonstrated the existence of AT2R and its transcript in human sperm by Western blot and reverse transcription–polymerase chain reaction. Immunofluorescence studies showed that AT2R is mainly located at the equatorial segment of the sperm head. The AT2R levels were associated with sperm motility parameters. Particularly, we found a significant positive correlation between AT2R and spermatozoa with progressive motility grade and a significant negative correlation with immotile spermatozoa, both in fresh semen samples and in prepared sperm cells. Regarding pathologic studies, the levels of AT2R measured by flow cytometry were lower in spermatozoa of asthenozoospermic men than in normozoospermic controls. Conclusion(s) Angiotensin II type 2 receptor is present in human semen and may be involved in the control of sperm motility. In-depth understanding of the proteins involved in sperm motility can help to elucidate the role of these proteins in male infertility as well as to establish new biomarkers for male infertility. |
| doi_str_mv | 10.1016/j.fertnstert.2015.11.004 |
| format | Article |
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Design We carried out expression assays for AT2R by reverse transcription–polymerase chain reaction, Western blot, immunofluorescence, and flow cytometry techniques in human sperm cells. Percentage of AT2R-positive sperm cells was analyzed by flow cytometry. Setting Assisted reproduction unit and academic research laboratory. Patient(s) Ninety-seven human semen samples from the Clínica IVI Bilbao. Intervention(s) All samples were examined and classified according to World Health Organization guidelines. Spermatozoa were isolated from semen on discontinuous colloidal silica gradient (45%–90%) and swim-up techniques. Main Outcome Measure(s) Presence and location of the AT2R in spermatozoa and percentage of AT2R-positive sperm cells measured by flow cytometry. Result(s) We demonstrated the existence of AT2R and its transcript in human sperm by Western blot and reverse transcription–polymerase chain reaction. Immunofluorescence studies showed that AT2R is mainly located at the equatorial segment of the sperm head. The AT2R levels were associated with sperm motility parameters. Particularly, we found a significant positive correlation between AT2R and spermatozoa with progressive motility grade and a significant negative correlation with immotile spermatozoa, both in fresh semen samples and in prepared sperm cells. Regarding pathologic studies, the levels of AT2R measured by flow cytometry were lower in spermatozoa of asthenozoospermic men than in normozoospermic controls. Conclusion(s) Angiotensin II type 2 receptor is present in human semen and may be involved in the control of sperm motility. In-depth understanding of the proteins involved in sperm motility can help to elucidate the role of these proteins in male infertility as well as to establish new biomarkers for male infertility.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2015.11.004</identifier><identifier>PMID: 26616438</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Angiotensin II type 2 receptor ; Asthenozoospermia - genetics ; Asthenozoospermia - metabolism ; Asthenozoospermia - pathology ; Asthenozoospermia - physiopathology ; Biomarkers - metabolism ; Blotting, Western ; Case-Control Studies ; Fertility ; Flow Cytometry ; Fluorescent Antibody Technique ; human ; Humans ; Internal Medicine ; Male ; male fertility ; Obstetrics and Gynecology ; Real-Time Polymerase Chain Reaction ; Receptor, Angiotensin, Type 2 - genetics ; Receptor, Angiotensin, Type 2 - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Signal Transduction ; sperm ; Sperm Motility ; Spermatozoa - metabolism ; Spermatozoa - pathology</subject><ispartof>Fertility and sterility, 2016-03, Vol.105 (3), p.608-616</ispartof><rights>American Society for Reproductive Medicine</rights><rights>2016 American Society for Reproductive Medicine</rights><rights>Copyright © 2016 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-99b820e7d2d54dc00f236c2f7d6f8e7b94dd23cae470d648fb334159bb9e12c33</citedby><cites>FETCH-LOGICAL-c549t-99b820e7d2d54dc00f236c2f7d6f8e7b94dd23cae470d648fb334159bb9e12c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0015028215020798$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26616438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gianzo, Marta, J.D</creatorcontrib><creatorcontrib>Muñoa-Hoyos, Iraia, J.D</creatorcontrib><creatorcontrib>Urizar-Arenaza, Itziar, J.D</creatorcontrib><creatorcontrib>Larreategui, Zaloa, J.D</creatorcontrib><creatorcontrib>Quintana, Fernando, J.D</creatorcontrib><creatorcontrib>Garrido, Nicolás, Ph.D</creatorcontrib><creatorcontrib>Subirán, Nerea, Ph.D</creatorcontrib><creatorcontrib>Irazusta, Jon, Ph.D</creatorcontrib><title>Angiotensin II type 2 receptor is expressed in human sperm cells and is involved in sperm motility</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective To investigate the presence of angiotensin II type 2 receptor (AT2R) in human spermatozoa and its implication in sperm fertility status. Design We carried out expression assays for AT2R by reverse transcription–polymerase chain reaction, Western blot, immunofluorescence, and flow cytometry techniques in human sperm cells. Percentage of AT2R-positive sperm cells was analyzed by flow cytometry. Setting Assisted reproduction unit and academic research laboratory. Patient(s) Ninety-seven human semen samples from the Clínica IVI Bilbao. Intervention(s) All samples were examined and classified according to World Health Organization guidelines. Spermatozoa were isolated from semen on discontinuous colloidal silica gradient (45%–90%) and swim-up techniques. Main Outcome Measure(s) Presence and location of the AT2R in spermatozoa and percentage of AT2R-positive sperm cells measured by flow cytometry. Result(s) We demonstrated the existence of AT2R and its transcript in human sperm by Western blot and reverse transcription–polymerase chain reaction. Immunofluorescence studies showed that AT2R is mainly located at the equatorial segment of the sperm head. The AT2R levels were associated with sperm motility parameters. Particularly, we found a significant positive correlation between AT2R and spermatozoa with progressive motility grade and a significant negative correlation with immotile spermatozoa, both in fresh semen samples and in prepared sperm cells. Regarding pathologic studies, the levels of AT2R measured by flow cytometry were lower in spermatozoa of asthenozoospermic men than in normozoospermic controls. Conclusion(s) Angiotensin II type 2 receptor is present in human semen and may be involved in the control of sperm motility. In-depth understanding of the proteins involved in sperm motility can help to elucidate the role of these proteins in male infertility as well as to establish new biomarkers for male infertility.</description><subject>Angiotensin II type 2 receptor</subject><subject>Asthenozoospermia - genetics</subject><subject>Asthenozoospermia - metabolism</subject><subject>Asthenozoospermia - pathology</subject><subject>Asthenozoospermia - physiopathology</subject><subject>Biomarkers - metabolism</subject><subject>Blotting, Western</subject><subject>Case-Control Studies</subject><subject>Fertility</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>human</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>male fertility</subject><subject>Obstetrics and Gynecology</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptor, Angiotensin, Type 2 - genetics</subject><subject>Receptor, Angiotensin, Type 2 - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal Transduction</subject><subject>sperm</subject><subject>Sperm Motility</subject><subject>Spermatozoa - metabolism</subject><subject>Spermatozoa - pathology</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkT2P1DAQQC0E4paDv4Bc0iSMncROGqTjdMBKJ1EAtZXYE_CS2MF2Vuy_x1EOkKhoxsW8-fAbQiiDkgETr0_liCG5mHIsObCmZKwEqB-RA2saUTSiqR6TA-RMAbzlV-RZjCcAEEzyp-SKC8FEXbUHMty4r9YndNE6ejzSdFmQchpQ45J8oDZS_LkEjBENzci3de4djQuGmWqcpkh7ZzbKurOfzju0p2ef7GTT5Tl5MvZTxBcP7zX58u7u8-2H4v7j--PtzX2hm7pLRdcNLQeUhpumNhpg5JXQfJRGjC3KoauN4ZXusZZgRN2OQ1XVrOmGoUPGdVVdk1d73yX4HyvGpGYbtx17h36NikkJrRSihYy2O6qDjzHgqJZg5z5cFAO1GVYn9dew2gwrxlQ2nEtfPkxZhxnNn8LfSjPwdgcw__VsMaioLTqNxmarSRlv_2fKm3-a6Mk6q_vpO14wnvwaXHapmIpcgfq0XXo79BZBdm31C3w4p9E</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Gianzo, Marta, J.D</creator><creator>Muñoa-Hoyos, Iraia, J.D</creator><creator>Urizar-Arenaza, Itziar, J.D</creator><creator>Larreategui, Zaloa, J.D</creator><creator>Quintana, Fernando, J.D</creator><creator>Garrido, Nicolás, Ph.D</creator><creator>Subirán, Nerea, Ph.D</creator><creator>Irazusta, Jon, Ph.D</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160301</creationdate><title>Angiotensin II type 2 receptor is expressed in human sperm cells and is involved in sperm motility</title><author>Gianzo, Marta, J.D ; Muñoa-Hoyos, Iraia, J.D ; Urizar-Arenaza, Itziar, J.D ; Larreategui, Zaloa, J.D ; Quintana, Fernando, J.D ; Garrido, Nicolás, Ph.D ; Subirán, Nerea, Ph.D ; Irazusta, Jon, Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-99b820e7d2d54dc00f236c2f7d6f8e7b94dd23cae470d648fb334159bb9e12c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Angiotensin II type 2 receptor</topic><topic>Asthenozoospermia - genetics</topic><topic>Asthenozoospermia - metabolism</topic><topic>Asthenozoospermia - pathology</topic><topic>Asthenozoospermia - physiopathology</topic><topic>Biomarkers - metabolism</topic><topic>Blotting, Western</topic><topic>Case-Control Studies</topic><topic>Fertility</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique</topic><topic>human</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>male fertility</topic><topic>Obstetrics and Gynecology</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptor, Angiotensin, Type 2 - genetics</topic><topic>Receptor, Angiotensin, Type 2 - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal Transduction</topic><topic>sperm</topic><topic>Sperm Motility</topic><topic>Spermatozoa - metabolism</topic><topic>Spermatozoa - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gianzo, Marta, J.D</creatorcontrib><creatorcontrib>Muñoa-Hoyos, Iraia, J.D</creatorcontrib><creatorcontrib>Urizar-Arenaza, Itziar, J.D</creatorcontrib><creatorcontrib>Larreategui, Zaloa, J.D</creatorcontrib><creatorcontrib>Quintana, Fernando, J.D</creatorcontrib><creatorcontrib>Garrido, Nicolás, Ph.D</creatorcontrib><creatorcontrib>Subirán, Nerea, Ph.D</creatorcontrib><creatorcontrib>Irazusta, Jon, Ph.D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gianzo, Marta, J.D</au><au>Muñoa-Hoyos, Iraia, J.D</au><au>Urizar-Arenaza, Itziar, J.D</au><au>Larreategui, Zaloa, J.D</au><au>Quintana, Fernando, J.D</au><au>Garrido, Nicolás, Ph.D</au><au>Subirán, Nerea, Ph.D</au><au>Irazusta, Jon, Ph.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiotensin II type 2 receptor is expressed in human sperm cells and is involved in sperm motility</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>105</volume><issue>3</issue><spage>608</spage><epage>616</epage><pages>608-616</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><abstract>Objective To investigate the presence of angiotensin II type 2 receptor (AT2R) in human spermatozoa and its implication in sperm fertility status. Design We carried out expression assays for AT2R by reverse transcription–polymerase chain reaction, Western blot, immunofluorescence, and flow cytometry techniques in human sperm cells. Percentage of AT2R-positive sperm cells was analyzed by flow cytometry. Setting Assisted reproduction unit and academic research laboratory. Patient(s) Ninety-seven human semen samples from the Clínica IVI Bilbao. Intervention(s) All samples were examined and classified according to World Health Organization guidelines. Spermatozoa were isolated from semen on discontinuous colloidal silica gradient (45%–90%) and swim-up techniques. Main Outcome Measure(s) Presence and location of the AT2R in spermatozoa and percentage of AT2R-positive sperm cells measured by flow cytometry. Result(s) We demonstrated the existence of AT2R and its transcript in human sperm by Western blot and reverse transcription–polymerase chain reaction. Immunofluorescence studies showed that AT2R is mainly located at the equatorial segment of the sperm head. The AT2R levels were associated with sperm motility parameters. Particularly, we found a significant positive correlation between AT2R and spermatozoa with progressive motility grade and a significant negative correlation with immotile spermatozoa, both in fresh semen samples and in prepared sperm cells. Regarding pathologic studies, the levels of AT2R measured by flow cytometry were lower in spermatozoa of asthenozoospermic men than in normozoospermic controls. Conclusion(s) Angiotensin II type 2 receptor is present in human semen and may be involved in the control of sperm motility. In-depth understanding of the proteins involved in sperm motility can help to elucidate the role of these proteins in male infertility as well as to establish new biomarkers for male infertility.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26616438</pmid><doi>10.1016/j.fertnstert.2015.11.004</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Angiotensin II type 2 receptor Asthenozoospermia - genetics Asthenozoospermia - metabolism Asthenozoospermia - pathology Asthenozoospermia - physiopathology Biomarkers - metabolism Blotting, Western Case-Control Studies Fertility Flow Cytometry Fluorescent Antibody Technique human Humans Internal Medicine Male male fertility Obstetrics and Gynecology Real-Time Polymerase Chain Reaction Receptor, Angiotensin, Type 2 - genetics Receptor, Angiotensin, Type 2 - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Signal Transduction sperm Sperm Motility Spermatozoa - metabolism Spermatozoa - pathology |
| title | Angiotensin II type 2 receptor is expressed in human sperm cells and is involved in sperm motility |
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