A composite agarose–polyacrylamide matrix as two-dimensional hard support for solid-phase protein assays
The solid-phase protein assays using blotting membranes as hard support do not allow achieving the low background and sensitivity of protein staining in clear gels. The membrane opacity complicates imaging of results on standard lab documentation systems. We describe a low-cost transparent matrix th...
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| Veröffentlicht in: | Analytical biochemistry 2016-03, Vol.497, p.57-59 |
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| container_title | Analytical biochemistry |
| container_volume | 497 |
| creator | Krajewski, Wladyslaw A. |
| description | The solid-phase protein assays using blotting membranes as hard support do not allow achieving the low background and sensitivity of protein staining in clear gels. The membrane opacity complicates imaging of results on standard lab documentation systems. We describe a low-cost transparent matrix that can be used as an alternative to polymeric membranes for solid-phase assays. Protein samples are spotted onto a dry film of composite agarose–polyacrylamide matrix covering standard glass microscopic slides. After rehydration in protein-fixing solution, matrix with protein samples can be detached from glass support and stained as conventional protein polyacrylamide gels. |
| doi_str_mv | 10.1016/j.ab.2015.11.023 |
| format | Article |
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The membrane opacity complicates imaging of results on standard lab documentation systems. We describe a low-cost transparent matrix that can be used as an alternative to polymeric membranes for solid-phase assays. Protein samples are spotted onto a dry film of composite agarose–polyacrylamide matrix covering standard glass microscopic slides. After rehydration in protein-fixing solution, matrix with protein samples can be detached from glass support and stained as conventional protein polyacrylamide gels.</description><subject>Acrylic Resins - chemistry</subject><subject>Animals</subject><subject>Cattle</subject><subject>Electrophoresis, Agar Gel</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Gel staining</subject><subject>Protein determination</subject><subject>Protein microassay</subject><subject>Proteins - analysis</subject><subject>Sepharose - chemistry</subject><subject>Serum Albumin, Bovine - analysis</subject><subject>Solid-phase assay</subject><subject>Staining and Labeling</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1u1TAQhS0Eai-l-64qL9kkjGPHabqrqvIjVWIDa2tiT1pfJXHqyS3cHe_AG_IkpNzCjtVsvnNG5xPiTEGpQNl32xK7sgJVl0qVUOkXYqOgtQVoaF-KDQDoorJtcyxeM28BlDK1PRLHlW3ANm27Edsr6dM4J44LSbzDnJh-_fg5p2GPPu8HHGMgOeKS43eJLJdvqQhxpIljmnCQ95iD5N08p7zIPmXJaYihmO-RSc45LRSnNce45zfiVY8D0-nzPRFf3998uf5Y3H7-8On66rbwuoal0AhgoCNtW6hCR31NvUVlgr5oOzLgiequ7ytAg30TyGIwxrYWjfVgtNYn4u2hd33_sCNe3BjZ0zDgRGnHTjUNXDQV_EHhgPp1N2fq3ZzjiHnvFLgnw27rsHNPhp1SbjW8Rs6f23fdSOFf4K_SFbg8ALRufIyUHftIk6cQM_nFhRT_3_4bLquOVQ</recordid><startdate>20160315</startdate><enddate>20160315</enddate><creator>Krajewski, Wladyslaw A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160315</creationdate><title>A composite agarose–polyacrylamide matrix as two-dimensional hard support for solid-phase protein assays</title><author>Krajewski, Wladyslaw A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-3a0040be36902dbef5ef6a14d389be40cee5bff20a4af7de6ad44696a46c04333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acrylic Resins - chemistry</topic><topic>Animals</topic><topic>Cattle</topic><topic>Electrophoresis, Agar Gel</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Gel staining</topic><topic>Protein determination</topic><topic>Protein microassay</topic><topic>Proteins - analysis</topic><topic>Sepharose - chemistry</topic><topic>Serum Albumin, Bovine - analysis</topic><topic>Solid-phase assay</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krajewski, Wladyslaw A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krajewski, Wladyslaw A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A composite agarose–polyacrylamide matrix as two-dimensional hard support for solid-phase protein assays</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2016-03-15</date><risdate>2016</risdate><volume>497</volume><spage>57</spage><epage>59</epage><pages>57-59</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>The solid-phase protein assays using blotting membranes as hard support do not allow achieving the low background and sensitivity of protein staining in clear gels. 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| ispartof | Analytical biochemistry, 2016-03, Vol.497, p.57-59 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1770872033 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Acrylic Resins - chemistry Animals Cattle Electrophoresis, Agar Gel Electrophoresis, Gel, Two-Dimensional Gel staining Protein determination Protein microassay Proteins - analysis Sepharose - chemistry Serum Albumin, Bovine - analysis Solid-phase assay Staining and Labeling |
| title | A composite agarose–polyacrylamide matrix as two-dimensional hard support for solid-phase protein assays |
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