A rapid UPLC-MS/MS method for the determination of oleanolic acid in rat plasma and liver tissue: application to plasma and liver pharmacokinetics

A reliable high‐throughput ultra‐high performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) method was developed and validated for oleanolic acid (OA) determination in rat plasma and liver tissue using glycyrrhetic acid as the internal standard (IS). Plasma and liver homogenate sam...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biomedical chromatography 2016-04, Vol.30 (4), p.520-527
Hauptverfasser: Li, Tian-xue, Chu, Chao-sen, Zhu, Jia-yu, Yang, Tian-yi, Zhang, Jie, Hu, Yu-tao, Yang, Xing-hao
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 527
container_issue 4
container_start_page 520
container_title Biomedical chromatography
container_volume 30
creator Li, Tian-xue
Chu, Chao-sen
Zhu, Jia-yu
Yang, Tian-yi
Zhang, Jie
Hu, Yu-tao
Yang, Xing-hao
description A reliable high‐throughput ultra‐high performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) method was developed and validated for oleanolic acid (OA) determination in rat plasma and liver tissue using glycyrrhetic acid as the internal standard (IS). Plasma and liver homogenate samples were prepared using solid‐phase extraction. Chromatographic separation was achieved on a C18 column using an isocratic mobile phase system. The detection was performed by multiple reaction monitoring mode via positive electrospray ionization interface. The calibration curves showed good linearity (R2 > 0.9997) within the tested concentration ranges. The lower limit of quantification for plasma and liver tissue was ≤0.75 ng/mL. The intra‐ and inter‐day precision and accuracy deviations were within ±15% in plasma and liver tissue. The mean extraction recoveries ranged from 80.8 to 87.0%. In addition, the carryover, matrix effect, stability and robustness involved in the method were also validated. The method was successfully applied to the plasma and hepatic pharmacokinetics of OA after oral administration to rats. Copyright © 2015 John Wiley & Sons, Ltd.
doi_str_mv 10.1002/bmc.3577
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1770861724</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1770861724</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4297-aac2b644da3cd37604a4ee575646355deb4e266b6463f8b72e3f01d5cf2d94183</originalsourceid><addsrcrecordid>eNp10c1u1DAUBWALgehQkHgC5CWbtP6LnbBrB2iRZgqoVCytG_tGY5rEqZ0B-ho8MRnNUBaIlXWl75yFDyEvOTvhjInTpncnsjTmEVlwVtcFqxh_TBZM6LqQlamPyLOcvzHGai3MU3IktJDKGLEgv85ogjF4evNptSzW16fra9rjtImetjHRaYPU44SpDwNMIQ40tjR2CEPsgqPg5mQY5oqJjh3kHigMnnbhO87ZkPMW31AYx9nu01P8140bSD24eBsGnILLz8mTFrqMLw7vMbl5_-7L8rJYfbz4sDxbFU6J2hQATjRaKQ_SeWk0U6AQS1NqpWVZemwUCq2b3dlWjREoW8Z96Vrha8UreUxe73vHFO-2mCfbh-yw62DAuM2WG8MqzY1Qf6lLMeeErR1T6CHdW87sbgE7L2B3C8z01aF12_ToH-CfL59BsQc_Qof3_y2y5-vlofDgQ57w54OHdGu1kaa0X68u7NVnreq3q3N7KX8DR8-fWA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1770861724</pqid></control><display><type>article</type><title>A rapid UPLC-MS/MS method for the determination of oleanolic acid in rat plasma and liver tissue: application to plasma and liver pharmacokinetics</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Li, Tian-xue ; Chu, Chao-sen ; Zhu, Jia-yu ; Yang, Tian-yi ; Zhang, Jie ; Hu, Yu-tao ; Yang, Xing-hao</creator><creatorcontrib>Li, Tian-xue ; Chu, Chao-sen ; Zhu, Jia-yu ; Yang, Tian-yi ; Zhang, Jie ; Hu, Yu-tao ; Yang, Xing-hao</creatorcontrib><description>A reliable high‐throughput ultra‐high performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) method was developed and validated for oleanolic acid (OA) determination in rat plasma and liver tissue using glycyrrhetic acid as the internal standard (IS). Plasma and liver homogenate samples were prepared using solid‐phase extraction. Chromatographic separation was achieved on a C18 column using an isocratic mobile phase system. The detection was performed by multiple reaction monitoring mode via positive electrospray ionization interface. The calibration curves showed good linearity (R2 &gt; 0.9997) within the tested concentration ranges. The lower limit of quantification for plasma and liver tissue was ≤0.75 ng/mL. The intra‐ and inter‐day precision and accuracy deviations were within ±15% in plasma and liver tissue. The mean extraction recoveries ranged from 80.8 to 87.0%. In addition, the carryover, matrix effect, stability and robustness involved in the method were also validated. The method was successfully applied to the plasma and hepatic pharmacokinetics of OA after oral administration to rats. Copyright © 2015 John Wiley &amp; Sons, Ltd.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.3577</identifier><identifier>PMID: 26234772</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Animals ; bio-analysis ; Chromatography, High Pressure Liquid - methods ; Limit of Detection ; Liquid-Liquid Extraction - methods ; Liver - chemistry ; liver tissue ; Male ; oleanolic acid ; Oleanolic Acid - analysis ; Oleanolic Acid - blood ; Rats ; Rats, Sprague-Dawley ; Reproducibility of Results ; solid-phase extraction ; Tandem Mass Spectrometry - methods ; UPLC-MS/MS</subject><ispartof>Biomedical chromatography, 2016-04, Vol.30 (4), p.520-527</ispartof><rights>Copyright © 2015 John Wiley &amp; Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4297-aac2b644da3cd37604a4ee575646355deb4e266b6463f8b72e3f01d5cf2d94183</citedby><cites>FETCH-LOGICAL-c4297-aac2b644da3cd37604a4ee575646355deb4e266b6463f8b72e3f01d5cf2d94183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmc.3577$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmc.3577$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26234772$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Tian-xue</creatorcontrib><creatorcontrib>Chu, Chao-sen</creatorcontrib><creatorcontrib>Zhu, Jia-yu</creatorcontrib><creatorcontrib>Yang, Tian-yi</creatorcontrib><creatorcontrib>Zhang, Jie</creatorcontrib><creatorcontrib>Hu, Yu-tao</creatorcontrib><creatorcontrib>Yang, Xing-hao</creatorcontrib><title>A rapid UPLC-MS/MS method for the determination of oleanolic acid in rat plasma and liver tissue: application to plasma and liver pharmacokinetics</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>A reliable high‐throughput ultra‐high performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) method was developed and validated for oleanolic acid (OA) determination in rat plasma and liver tissue using glycyrrhetic acid as the internal standard (IS). Plasma and liver homogenate samples were prepared using solid‐phase extraction. Chromatographic separation was achieved on a C18 column using an isocratic mobile phase system. The detection was performed by multiple reaction monitoring mode via positive electrospray ionization interface. The calibration curves showed good linearity (R2 &gt; 0.9997) within the tested concentration ranges. The lower limit of quantification for plasma and liver tissue was ≤0.75 ng/mL. The intra‐ and inter‐day precision and accuracy deviations were within ±15% in plasma and liver tissue. The mean extraction recoveries ranged from 80.8 to 87.0%. In addition, the carryover, matrix effect, stability and robustness involved in the method were also validated. The method was successfully applied to the plasma and hepatic pharmacokinetics of OA after oral administration to rats. Copyright © 2015 John Wiley &amp; Sons, Ltd.</description><subject>Animals</subject><subject>bio-analysis</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Limit of Detection</subject><subject>Liquid-Liquid Extraction - methods</subject><subject>Liver - chemistry</subject><subject>liver tissue</subject><subject>Male</subject><subject>oleanolic acid</subject><subject>Oleanolic Acid - analysis</subject><subject>Oleanolic Acid - blood</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reproducibility of Results</subject><subject>solid-phase extraction</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>UPLC-MS/MS</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10c1u1DAUBWALgehQkHgC5CWbtP6LnbBrB2iRZgqoVCytG_tGY5rEqZ0B-ho8MRnNUBaIlXWl75yFDyEvOTvhjInTpncnsjTmEVlwVtcFqxh_TBZM6LqQlamPyLOcvzHGai3MU3IktJDKGLEgv85ogjF4evNptSzW16fra9rjtImetjHRaYPU44SpDwNMIQ40tjR2CEPsgqPg5mQY5oqJjh3kHigMnnbhO87ZkPMW31AYx9nu01P8140bSD24eBsGnILLz8mTFrqMLw7vMbl5_-7L8rJYfbz4sDxbFU6J2hQATjRaKQ_SeWk0U6AQS1NqpWVZemwUCq2b3dlWjREoW8Z96Vrha8UreUxe73vHFO-2mCfbh-yw62DAuM2WG8MqzY1Qf6lLMeeErR1T6CHdW87sbgE7L2B3C8z01aF12_ToH-CfL59BsQc_Qof3_y2y5-vlofDgQ57w54OHdGu1kaa0X68u7NVnreq3q3N7KX8DR8-fWA</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Li, Tian-xue</creator><creator>Chu, Chao-sen</creator><creator>Zhu, Jia-yu</creator><creator>Yang, Tian-yi</creator><creator>Zhang, Jie</creator><creator>Hu, Yu-tao</creator><creator>Yang, Xing-hao</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201604</creationdate><title>A rapid UPLC-MS/MS method for the determination of oleanolic acid in rat plasma and liver tissue: application to plasma and liver pharmacokinetics</title><author>Li, Tian-xue ; Chu, Chao-sen ; Zhu, Jia-yu ; Yang, Tian-yi ; Zhang, Jie ; Hu, Yu-tao ; Yang, Xing-hao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4297-aac2b644da3cd37604a4ee575646355deb4e266b6463f8b72e3f01d5cf2d94183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>bio-analysis</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Limit of Detection</topic><topic>Liquid-Liquid Extraction - methods</topic><topic>Liver - chemistry</topic><topic>liver tissue</topic><topic>Male</topic><topic>oleanolic acid</topic><topic>Oleanolic Acid - analysis</topic><topic>Oleanolic Acid - blood</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reproducibility of Results</topic><topic>solid-phase extraction</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>UPLC-MS/MS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Tian-xue</creatorcontrib><creatorcontrib>Chu, Chao-sen</creatorcontrib><creatorcontrib>Zhu, Jia-yu</creatorcontrib><creatorcontrib>Yang, Tian-yi</creatorcontrib><creatorcontrib>Zhang, Jie</creatorcontrib><creatorcontrib>Hu, Yu-tao</creatorcontrib><creatorcontrib>Yang, Xing-hao</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Tian-xue</au><au>Chu, Chao-sen</au><au>Zhu, Jia-yu</au><au>Yang, Tian-yi</au><au>Zhang, Jie</au><au>Hu, Yu-tao</au><au>Yang, Xing-hao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid UPLC-MS/MS method for the determination of oleanolic acid in rat plasma and liver tissue: application to plasma and liver pharmacokinetics</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>2016-04</date><risdate>2016</risdate><volume>30</volume><issue>4</issue><spage>520</spage><epage>527</epage><pages>520-527</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>A reliable high‐throughput ultra‐high performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) method was developed and validated for oleanolic acid (OA) determination in rat plasma and liver tissue using glycyrrhetic acid as the internal standard (IS). Plasma and liver homogenate samples were prepared using solid‐phase extraction. Chromatographic separation was achieved on a C18 column using an isocratic mobile phase system. The detection was performed by multiple reaction monitoring mode via positive electrospray ionization interface. The calibration curves showed good linearity (R2 &gt; 0.9997) within the tested concentration ranges. The lower limit of quantification for plasma and liver tissue was ≤0.75 ng/mL. The intra‐ and inter‐day precision and accuracy deviations were within ±15% in plasma and liver tissue. The mean extraction recoveries ranged from 80.8 to 87.0%. In addition, the carryover, matrix effect, stability and robustness involved in the method were also validated. The method was successfully applied to the plasma and hepatic pharmacokinetics of OA after oral administration to rats. Copyright © 2015 John Wiley &amp; Sons, Ltd.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>26234772</pmid><doi>10.1002/bmc.3577</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0269-3879
ispartof Biomedical chromatography, 2016-04, Vol.30 (4), p.520-527
issn 0269-3879
1099-0801
language eng
recordid cdi_proquest_miscellaneous_1770861724
source MEDLINE; Access via Wiley Online Library
subjects Animals
bio-analysis
Chromatography, High Pressure Liquid - methods
Limit of Detection
Liquid-Liquid Extraction - methods
Liver - chemistry
liver tissue
Male
oleanolic acid
Oleanolic Acid - analysis
Oleanolic Acid - blood
Rats
Rats, Sprague-Dawley
Reproducibility of Results
solid-phase extraction
Tandem Mass Spectrometry - methods
UPLC-MS/MS
title A rapid UPLC-MS/MS method for the determination of oleanolic acid in rat plasma and liver tissue: application to plasma and liver pharmacokinetics
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T21%3A56%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20rapid%20UPLC-MS/MS%20method%20for%20the%20determination%20of%20oleanolic%20acid%20in%20rat%20plasma%20and%20liver%20tissue:%20application%20to%20plasma%20and%20liver%20pharmacokinetics&rft.jtitle=Biomedical%20chromatography&rft.au=Li,%20Tian-xue&rft.date=2016-04&rft.volume=30&rft.issue=4&rft.spage=520&rft.epage=527&rft.pages=520-527&rft.issn=0269-3879&rft.eissn=1099-0801&rft_id=info:doi/10.1002/bmc.3577&rft_dat=%3Cproquest_cross%3E1770861724%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1770861724&rft_id=info:pmid/26234772&rfr_iscdi=true