Regulation of nestin expression after cortical ablation in adult rat brain
During embryogenesis, transient expression of nestin in proliferating neuroepithelial stem cells signals the commitment of progenitor cells to differentiate. Although adult mammalian brain contains very little nestin, significant upregulation of nestin has been reported following cerebral injury, le...
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| Veröffentlicht in: | Brain research 2004-05, Vol.1008 (2), p.139-146 |
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| creator | Douen, A.G Dong, Li Vanance, S Munger, R Hogan, M.J Thompson, C.S Hakim, A.M |
| description | During embryogenesis, transient expression of nestin in proliferating neuroepithelial stem cells signals the commitment of progenitor cells to differentiate. Although adult mammalian brain contains very little nestin, significant upregulation of nestin has been reported following cerebral injury, leading to speculation that nestin may be involved in brain repair. In this study, we assessed the temporal profile of nestin expression following ablation injury of the sensory barrel cortex and investigated the influence of contralateral whisker stimulation on nestin expression. Since the adult mammalian brain contains proliferating neuronal progenitor cells that can be labeled with bromodeoxyuridine (BrdU), we also determined the association of nestin reexpression with BrdU-labeled cells. Nestin reexpression was detected predominantly in the ipsilateral cortex 3 days post-ablation. There was no significant nestin upregulation in the subcortical region. Nestin reexpression was most marked surrounding the lesion, but also extended throughout the entire lateral cortex. Nestin in the ipsilateral cortex subsided by day 7, although perilesional nestin expression was still apparent 28 days post-injury. Western blot analysis of nestin expression 3 days post-ablation confirmed a significant two-fold increase in nestin expression (
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| doi_str_mv | 10.1016/j.brainres.2003.08.070 |
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p<0.05). Double immunofluorescence labeling demonstrated that the majority of nestin expression occurred in astrocytes. We were unable to detect any colocalization with neuronal makers. However, BrdU-labeled cells, which were readily detected in the subventricular zone prior to injury, were readily detected in the perilesional area 3 days post-ablation, concomitant with nestin in this area. Confocal microscopy detected several BrdU-positive cells expressing nestin. Taken together, the data support a potential role for nestin reexpression in brain repair.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2003.08.070</identifier><identifier>PMID: 15145750</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Antimetabolites ; Biological and medical sciences ; Blotting, Western ; Brain Chemistry - physiology ; Brain repair ; BrdU ; Bromodeoxyuridine ; Cerebral Cortex - physiology ; Cortical ablation ; Deoxyglucose - pharmacology ; Development. Senescence. Regeneration. Transplantation ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Indicators and Reagents ; Intermediate Filament Proteins - biosynthesis ; Male ; Microscopy, Confocal ; Motor Cortex - physiology ; Nerve Regeneration - physiology ; Nerve Tissue Proteins - biosynthesis ; Nestin ; Physical Stimulation ; Rats ; Rats, Wistar ; Stem cell ; Stem Cells - physiology ; Vertebrates: nervous system and sense organs ; Vibrissae - physiology</subject><ispartof>Brain research, 2004-05, Vol.1008 (2), p.139-146</ispartof><rights>2004</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-d4f651efe02313c5c9de6b95dc5074176b7e3d21c291763c55da0e02388516ca3</citedby><cites>FETCH-LOGICAL-c460t-d4f651efe02313c5c9de6b95dc5074176b7e3d21c291763c55da0e02388516ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006899304002331$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15778843$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15145750$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Douen, A.G</creatorcontrib><creatorcontrib>Dong, Li</creatorcontrib><creatorcontrib>Vanance, S</creatorcontrib><creatorcontrib>Munger, R</creatorcontrib><creatorcontrib>Hogan, M.J</creatorcontrib><creatorcontrib>Thompson, C.S</creatorcontrib><creatorcontrib>Hakim, A.M</creatorcontrib><title>Regulation of nestin expression after cortical ablation in adult rat brain</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>During embryogenesis, transient expression of nestin in proliferating neuroepithelial stem cells signals the commitment of progenitor cells to differentiate. Although adult mammalian brain contains very little nestin, significant upregulation of nestin has been reported following cerebral injury, leading to speculation that nestin may be involved in brain repair. In this study, we assessed the temporal profile of nestin expression following ablation injury of the sensory barrel cortex and investigated the influence of contralateral whisker stimulation on nestin expression. Since the adult mammalian brain contains proliferating neuronal progenitor cells that can be labeled with bromodeoxyuridine (BrdU), we also determined the association of nestin reexpression with BrdU-labeled cells. Nestin reexpression was detected predominantly in the ipsilateral cortex 3 days post-ablation. There was no significant nestin upregulation in the subcortical region. Nestin reexpression was most marked surrounding the lesion, but also extended throughout the entire lateral cortex. Nestin in the ipsilateral cortex subsided by day 7, although perilesional nestin expression was still apparent 28 days post-injury. Western blot analysis of nestin expression 3 days post-ablation confirmed a significant two-fold increase in nestin expression (
p<0.05). Double immunofluorescence labeling demonstrated that the majority of nestin expression occurred in astrocytes. We were unable to detect any colocalization with neuronal makers. However, BrdU-labeled cells, which were readily detected in the subventricular zone prior to injury, were readily detected in the perilesional area 3 days post-ablation, concomitant with nestin in this area. Confocal microscopy detected several BrdU-positive cells expressing nestin. Taken together, the data support a potential role for nestin reexpression in brain repair.</description><subject>Animals</subject><subject>Antimetabolites</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brain Chemistry - physiology</subject><subject>Brain repair</subject><subject>BrdU</subject><subject>Bromodeoxyuridine</subject><subject>Cerebral Cortex - physiology</subject><subject>Cortical ablation</subject><subject>Deoxyglucose - pharmacology</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Indicators and Reagents</subject><subject>Intermediate Filament Proteins - biosynthesis</subject><subject>Male</subject><subject>Microscopy, Confocal</subject><subject>Motor Cortex - physiology</subject><subject>Nerve Regeneration - physiology</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Nestin</subject><subject>Physical Stimulation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Stem cell</subject><subject>Stem Cells - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Vibrissae - physiology</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo7vrxF5Ze9NY6aZuP3hTxE0EQPYc0mUqWbrsmrei_N-tW9OZpmOGZmZeHkAWFjALlZ8us9tp1HkOWAxQZyAwE7JA5lSJPeV7CLpkDAE9lVRUzchDCMrZFUcE-mVFGSyYYzMn9E76OrR5c3yV9k3QYBtcl-LGOl8NmqJsBfWJ6Pzij20TXExwpbcd2SLweku8sR2Sv0W3A46kekpfrq-fL2_Th8ebu8uIhNSWHIbVlwxnFBiEvaGGYqSzyumLWMBAlFbwWWNicmryKTQSY1bCBpWSUG10cktPt3bXv38YYWK1cMNi2usN-DIoKATKXZQT5FjS-D8Fjo9berbT_VBTUxqJaqh-LamNRgVTRYlxcTB_GeoX2d23SFoGTCdAhWmm87owLfzghZAwQufMth9HHu0OvgnHYGbTOoxmU7d1_Wb4AVkWTow</recordid><startdate>20040522</startdate><enddate>20040522</enddate><creator>Douen, A.G</creator><creator>Dong, Li</creator><creator>Vanance, S</creator><creator>Munger, R</creator><creator>Hogan, M.J</creator><creator>Thompson, C.S</creator><creator>Hakim, A.M</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20040522</creationdate><title>Regulation of nestin expression after cortical ablation in adult rat brain</title><author>Douen, A.G ; Dong, Li ; Vanance, S ; Munger, R ; Hogan, M.J ; Thompson, C.S ; Hakim, A.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-d4f651efe02313c5c9de6b95dc5074176b7e3d21c291763c55da0e02388516ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antimetabolites</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Brain Chemistry - physiology</topic><topic>Brain repair</topic><topic>BrdU</topic><topic>Bromodeoxyuridine</topic><topic>Cerebral Cortex - physiology</topic><topic>Cortical ablation</topic><topic>Deoxyglucose - pharmacology</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Indicators and Reagents</topic><topic>Intermediate Filament Proteins - biosynthesis</topic><topic>Male</topic><topic>Microscopy, Confocal</topic><topic>Motor Cortex - physiology</topic><topic>Nerve Regeneration - physiology</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nestin</topic><topic>Physical Stimulation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Stem cell</topic><topic>Stem Cells - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Vibrissae - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Douen, A.G</creatorcontrib><creatorcontrib>Dong, Li</creatorcontrib><creatorcontrib>Vanance, S</creatorcontrib><creatorcontrib>Munger, R</creatorcontrib><creatorcontrib>Hogan, M.J</creatorcontrib><creatorcontrib>Thompson, C.S</creatorcontrib><creatorcontrib>Hakim, A.M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Douen, A.G</au><au>Dong, Li</au><au>Vanance, S</au><au>Munger, R</au><au>Hogan, M.J</au><au>Thompson, C.S</au><au>Hakim, A.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of nestin expression after cortical ablation in adult rat brain</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2004-05-22</date><risdate>2004</risdate><volume>1008</volume><issue>2</issue><spage>139</spage><epage>146</epage><pages>139-146</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>During embryogenesis, transient expression of nestin in proliferating neuroepithelial stem cells signals the commitment of progenitor cells to differentiate. Although adult mammalian brain contains very little nestin, significant upregulation of nestin has been reported following cerebral injury, leading to speculation that nestin may be involved in brain repair. In this study, we assessed the temporal profile of nestin expression following ablation injury of the sensory barrel cortex and investigated the influence of contralateral whisker stimulation on nestin expression. Since the adult mammalian brain contains proliferating neuronal progenitor cells that can be labeled with bromodeoxyuridine (BrdU), we also determined the association of nestin reexpression with BrdU-labeled cells. Nestin reexpression was detected predominantly in the ipsilateral cortex 3 days post-ablation. There was no significant nestin upregulation in the subcortical region. Nestin reexpression was most marked surrounding the lesion, but also extended throughout the entire lateral cortex. Nestin in the ipsilateral cortex subsided by day 7, although perilesional nestin expression was still apparent 28 days post-injury. Western blot analysis of nestin expression 3 days post-ablation confirmed a significant two-fold increase in nestin expression (
p<0.05). Double immunofluorescence labeling demonstrated that the majority of nestin expression occurred in astrocytes. We were unable to detect any colocalization with neuronal makers. However, BrdU-labeled cells, which were readily detected in the subventricular zone prior to injury, were readily detected in the perilesional area 3 days post-ablation, concomitant with nestin in this area. Confocal microscopy detected several BrdU-positive cells expressing nestin. Taken together, the data support a potential role for nestin reexpression in brain repair.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>15145750</pmid><doi>10.1016/j.brainres.2003.08.070</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antimetabolites Biological and medical sciences Blotting, Western Brain Chemistry - physiology Brain repair BrdU Bromodeoxyuridine Cerebral Cortex - physiology Cortical ablation Deoxyglucose - pharmacology Development. Senescence. Regeneration. Transplantation Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Immunohistochemistry Indicators and Reagents Intermediate Filament Proteins - biosynthesis Male Microscopy, Confocal Motor Cortex - physiology Nerve Regeneration - physiology Nerve Tissue Proteins - biosynthesis Nestin Physical Stimulation Rats Rats, Wistar Stem cell Stem Cells - physiology Vertebrates: nervous system and sense organs Vibrissae - physiology |
| title | Regulation of nestin expression after cortical ablation in adult rat brain |
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