Biochemical characterization of Aspergillus awamori exoinulinase: substrate binding characteristics and regioselectivity of hydrolysis

1H-NMR analysis was applied to investigate the hydrolytic activity of Aspergillus awamori inulinase. The obtained NMR signals and deduced metabolite pattern revealed that the enzyme cleaves off only fructose from inulin and does not possess transglycosylating activity. Kinetics for the enzyme hydrol...

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Veröffentlicht in:	Biochimica et biophysica acta 2003-08, Vol.1650 (1), p.22-29
Hauptverfasser:	Kulminskaya, Anna A., Arand, Michael, Eneyskaya, Elena V., Ivanen, Dina R., Shabalin, Konstantin A., Shishlyannikov, Sergei M., Saveliev, Andrew N., Korneeva, Olga S., Neustroev, Kirill N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspergillus - chemistry Aspergillus - enzymology Aspergillus - metabolism Aspergillus awamori Binding Sites Exoinulinase Fructooligosaccharide Fructosyl-binding site Glycoside Hydrolases - chemistry Glycoside Hydrolases - genetics Glycoside Hydrolases - metabolism Hydrolysis Inulin - chemistry Magnetic Resonance Spectroscopy Multiple attack Time Factors
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