Characterization and dye decolorization ability of an alkaline resistant and organic solvents tolerant laccase from Bacillus licheniformis LS04
► A novel bacterial strain exhibiting laccase activity was isolated. ► The strain was identified as Bacillus licheniformis LS04. ► The spore laccase from strain LS04 was quite stable at 80°C and pH 9.0. ► The spore laccase was also tolerant towards 1M NaCl and 30% of organic solvents. ► More than 80...
Gespeichert in:
| Veröffentlicht in: | Bioresource technology 2012-07, Vol.115, p.35-40 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 40 |
|---|---|
| container_issue | |
| container_start_page | 35 |
| container_title | Bioresource technology |
| container_volume | 115 |
| creator | Lu, Lei Zhao, Min Wang, Tian-Nv Zhao, Li-Yan Du, Mei-Hui Li, Tai-Lun Li, De-Bin |
| description | ► A novel bacterial strain exhibiting laccase activity was isolated. ► The strain was identified as Bacillus licheniformis LS04. ► The spore laccase from strain LS04 was quite stable at 80°C and pH 9.0. ► The spore laccase was also tolerant towards 1M NaCl and 30% of organic solvents. ► More than 80% of dye decolorization was achieved in 1h with acetosyringone as mediator.
A new bacterial strain exhibiting laccase activity was isolated from forest soil and was identified as Bacillus licheniformis LS04. The spore laccase of B. licheniformis LS04 demonstrated a broad pH range for catalyzing substrates. It was quite stable at high temperature and alkaline pH. There was no loss of laccase activity after 10days incubation at pH 9.0, and about 16% of the initial activity was detected after 10h at 80°C. In addition, the spore laccase was tolerant towards 1M of NaCl and 30% of organic solvents. Reactive black 5, reactive blue 19 and indigo carmine were decolorized by the spore laccase in the absence of mediator. Meanwhile, the decolorization process was efficiently promoted when acetosyringone was present, with more than 80% of color removal in 1h at pH 6.6 or 9.0. The unusual properties indicated a high potential in industrial applications for this novel spore laccase. |
| doi_str_mv | 10.1016/j.biortech.2011.07.111 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1770374768</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0960852411010790</els_id><sourcerecordid>1038617591</sourcerecordid><originalsourceid>FETCH-LOGICAL-c500t-ad04ffd5313a44a0714f63766a5fc84516b19f5da98268011acb7f328b48aef93</originalsourceid><addsrcrecordid>eNqNkc9uEzEQxi0EoqHwCpWPXHax17u29wZE5Y8UiQNwtma9Y-LgrIvtVAovwSvjkBaO5TTSzO-bGX0fIVectZxx-WrXTj6mgnbbdozzlqmWc_6IrLhWoulGJR-TFRsla_TQ9RfkWc47xpjgqntKLjqupe64WpFf6y0ksAWT_wnFx4XCMtP5iHRGG0P815588OVIo6sEhfAdgl-QJsw-F1jKH11M32DxluYYbnEpmZYYMJ2mAayFjNSluKdvwfoQDpkGb7e4eBfT3me6-cz65-SJg5DxxV29JF_fXX9Zf2g2n95_XL_ZNHZgrDQws965eRBcQN8DU7x3UigpYXBW9wOXEx_dMMOoO6mrQWAn5USnp14DulFckpfnvTcp_jhgLqZ-YDEEWDAesuFKMaF6JfXDKBNacjWM_D_QulcKJrqKyjNqU8w5oTM3ye8hHSt04qTZmfuIzSliw5SpEVfh1d2Nw7TH-a_sPtMKvD4DWP279ZhMth4Xi7NPaIuZo3_oxm8ohrz4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1017763032</pqid></control><display><type>article</type><title>Characterization and dye decolorization ability of an alkaline resistant and organic solvents tolerant laccase from Bacillus licheniformis LS04</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Lu, Lei ; Zhao, Min ; Wang, Tian-Nv ; Zhao, Li-Yan ; Du, Mei-Hui ; Li, Tai-Lun ; Li, De-Bin</creator><creatorcontrib>Lu, Lei ; Zhao, Min ; Wang, Tian-Nv ; Zhao, Li-Yan ; Du, Mei-Hui ; Li, Tai-Lun ; Li, De-Bin</creatorcontrib><description>► A novel bacterial strain exhibiting laccase activity was isolated. ► The strain was identified as Bacillus licheniformis LS04. ► The spore laccase from strain LS04 was quite stable at 80°C and pH 9.0. ► The spore laccase was also tolerant towards 1M NaCl and 30% of organic solvents. ► More than 80% of dye decolorization was achieved in 1h with acetosyringone as mediator.
A new bacterial strain exhibiting laccase activity was isolated from forest soil and was identified as Bacillus licheniformis LS04. The spore laccase of B. licheniformis LS04 demonstrated a broad pH range for catalyzing substrates. It was quite stable at high temperature and alkaline pH. There was no loss of laccase activity after 10days incubation at pH 9.0, and about 16% of the initial activity was detected after 10h at 80°C. In addition, the spore laccase was tolerant towards 1M of NaCl and 30% of organic solvents. Reactive black 5, reactive blue 19 and indigo carmine were decolorized by the spore laccase in the absence of mediator. Meanwhile, the decolorization process was efficiently promoted when acetosyringone was present, with more than 80% of color removal in 1h at pH 6.6 or 9.0. The unusual properties indicated a high potential in industrial applications for this novel spore laccase.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2011.07.111</identifier><identifier>PMID: 21868217</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adaptation, Physiological - drug effects ; Alkalies - pharmacology ; Bacillus - drug effects ; Bacillus - enzymology ; Bacillus - genetics ; Bacillus - isolation & purification ; Bacillus licheniformis ; Bacteria ; Base Sequence ; Biodegradation, Environmental - drug effects ; Characterization ; Color ; Coloring Agents - isolation & purification ; Decoloring ; Decolorization ; Enzyme Inhibitors - pharmacology ; Enzyme Stability - drug effects ; Hydrogen-Ion Concentration - drug effects ; Isolation ; Laccase ; Laccase - metabolism ; Organic Chemicals - pharmacology ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Solvents ; Solvents - pharmacology ; Spores ; Spores, Bacterial - enzymology ; Substrate Specificity - drug effects ; Temperature</subject><ispartof>Bioresource technology, 2012-07, Vol.115, p.35-40</ispartof><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-ad04ffd5313a44a0714f63766a5fc84516b19f5da98268011acb7f328b48aef93</citedby><cites>FETCH-LOGICAL-c500t-ad04ffd5313a44a0714f63766a5fc84516b19f5da98268011acb7f328b48aef93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852411010790$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21868217$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Lei</creatorcontrib><creatorcontrib>Zhao, Min</creatorcontrib><creatorcontrib>Wang, Tian-Nv</creatorcontrib><creatorcontrib>Zhao, Li-Yan</creatorcontrib><creatorcontrib>Du, Mei-Hui</creatorcontrib><creatorcontrib>Li, Tai-Lun</creatorcontrib><creatorcontrib>Li, De-Bin</creatorcontrib><title>Characterization and dye decolorization ability of an alkaline resistant and organic solvents tolerant laccase from Bacillus licheniformis LS04</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>► A novel bacterial strain exhibiting laccase activity was isolated. ► The strain was identified as Bacillus licheniformis LS04. ► The spore laccase from strain LS04 was quite stable at 80°C and pH 9.0. ► The spore laccase was also tolerant towards 1M NaCl and 30% of organic solvents. ► More than 80% of dye decolorization was achieved in 1h with acetosyringone as mediator.
A new bacterial strain exhibiting laccase activity was isolated from forest soil and was identified as Bacillus licheniformis LS04. The spore laccase of B. licheniformis LS04 demonstrated a broad pH range for catalyzing substrates. It was quite stable at high temperature and alkaline pH. There was no loss of laccase activity after 10days incubation at pH 9.0, and about 16% of the initial activity was detected after 10h at 80°C. In addition, the spore laccase was tolerant towards 1M of NaCl and 30% of organic solvents. Reactive black 5, reactive blue 19 and indigo carmine were decolorized by the spore laccase in the absence of mediator. Meanwhile, the decolorization process was efficiently promoted when acetosyringone was present, with more than 80% of color removal in 1h at pH 6.6 or 9.0. The unusual properties indicated a high potential in industrial applications for this novel spore laccase.</description><subject>Adaptation, Physiological - drug effects</subject><subject>Alkalies - pharmacology</subject><subject>Bacillus - drug effects</subject><subject>Bacillus - enzymology</subject><subject>Bacillus - genetics</subject><subject>Bacillus - isolation & purification</subject><subject>Bacillus licheniformis</subject><subject>Bacteria</subject><subject>Base Sequence</subject><subject>Biodegradation, Environmental - drug effects</subject><subject>Characterization</subject><subject>Color</subject><subject>Coloring Agents - isolation & purification</subject><subject>Decoloring</subject><subject>Decolorization</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Enzyme Stability - drug effects</subject><subject>Hydrogen-Ion Concentration - drug effects</subject><subject>Isolation</subject><subject>Laccase</subject><subject>Laccase - metabolism</subject><subject>Organic Chemicals - pharmacology</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Solvents</subject><subject>Solvents - pharmacology</subject><subject>Spores</subject><subject>Spores, Bacterial - enzymology</subject><subject>Substrate Specificity - drug effects</subject><subject>Temperature</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9uEzEQxi0EoqHwCpWPXHax17u29wZE5Y8UiQNwtma9Y-LgrIvtVAovwSvjkBaO5TTSzO-bGX0fIVectZxx-WrXTj6mgnbbdozzlqmWc_6IrLhWoulGJR-TFRsla_TQ9RfkWc47xpjgqntKLjqupe64WpFf6y0ksAWT_wnFx4XCMtP5iHRGG0P815588OVIo6sEhfAdgl-QJsw-F1jKH11M32DxluYYbnEpmZYYMJ2mAayFjNSluKdvwfoQDpkGb7e4eBfT3me6-cz65-SJg5DxxV29JF_fXX9Zf2g2n95_XL_ZNHZgrDQws965eRBcQN8DU7x3UigpYXBW9wOXEx_dMMOoO6mrQWAn5USnp14DulFckpfnvTcp_jhgLqZ-YDEEWDAesuFKMaF6JfXDKBNacjWM_D_QulcKJrqKyjNqU8w5oTM3ye8hHSt04qTZmfuIzSliw5SpEVfh1d2Nw7TH-a_sPtMKvD4DWP279ZhMth4Xi7NPaIuZo3_oxm8ohrz4</recordid><startdate>201207</startdate><enddate>201207</enddate><creator>Lu, Lei</creator><creator>Zhao, Min</creator><creator>Wang, Tian-Nv</creator><creator>Zhao, Li-Yan</creator><creator>Du, Mei-Hui</creator><creator>Li, Tai-Lun</creator><creator>Li, De-Bin</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7SU</scope><scope>7TB</scope><scope>KR7</scope></search><sort><creationdate>201207</creationdate><title>Characterization and dye decolorization ability of an alkaline resistant and organic solvents tolerant laccase from Bacillus licheniformis LS04</title><author>Lu, Lei ; Zhao, Min ; Wang, Tian-Nv ; Zhao, Li-Yan ; Du, Mei-Hui ; Li, Tai-Lun ; Li, De-Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-ad04ffd5313a44a0714f63766a5fc84516b19f5da98268011acb7f328b48aef93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adaptation, Physiological - drug effects</topic><topic>Alkalies - pharmacology</topic><topic>Bacillus - drug effects</topic><topic>Bacillus - enzymology</topic><topic>Bacillus - genetics</topic><topic>Bacillus - isolation & purification</topic><topic>Bacillus licheniformis</topic><topic>Bacteria</topic><topic>Base Sequence</topic><topic>Biodegradation, Environmental - drug effects</topic><topic>Characterization</topic><topic>Color</topic><topic>Coloring Agents - isolation & purification</topic><topic>Decoloring</topic><topic>Decolorization</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Enzyme Stability - drug effects</topic><topic>Hydrogen-Ion Concentration - drug effects</topic><topic>Isolation</topic><topic>Laccase</topic><topic>Laccase - metabolism</topic><topic>Organic Chemicals - pharmacology</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Solvents</topic><topic>Solvents - pharmacology</topic><topic>Spores</topic><topic>Spores, Bacterial - enzymology</topic><topic>Substrate Specificity - drug effects</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Lei</creatorcontrib><creatorcontrib>Zhao, Min</creatorcontrib><creatorcontrib>Wang, Tian-Nv</creatorcontrib><creatorcontrib>Zhao, Li-Yan</creatorcontrib><creatorcontrib>Du, Mei-Hui</creatorcontrib><creatorcontrib>Li, Tai-Lun</creatorcontrib><creatorcontrib>Li, De-Bin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Lei</au><au>Zhao, Min</au><au>Wang, Tian-Nv</au><au>Zhao, Li-Yan</au><au>Du, Mei-Hui</au><au>Li, Tai-Lun</au><au>Li, De-Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and dye decolorization ability of an alkaline resistant and organic solvents tolerant laccase from Bacillus licheniformis LS04</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2012-07</date><risdate>2012</risdate><volume>115</volume><spage>35</spage><epage>40</epage><pages>35-40</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>► A novel bacterial strain exhibiting laccase activity was isolated. ► The strain was identified as Bacillus licheniformis LS04. ► The spore laccase from strain LS04 was quite stable at 80°C and pH 9.0. ► The spore laccase was also tolerant towards 1M NaCl and 30% of organic solvents. ► More than 80% of dye decolorization was achieved in 1h with acetosyringone as mediator.
A new bacterial strain exhibiting laccase activity was isolated from forest soil and was identified as Bacillus licheniformis LS04. The spore laccase of B. licheniformis LS04 demonstrated a broad pH range for catalyzing substrates. It was quite stable at high temperature and alkaline pH. There was no loss of laccase activity after 10days incubation at pH 9.0, and about 16% of the initial activity was detected after 10h at 80°C. In addition, the spore laccase was tolerant towards 1M of NaCl and 30% of organic solvents. Reactive black 5, reactive blue 19 and indigo carmine were decolorized by the spore laccase in the absence of mediator. Meanwhile, the decolorization process was efficiently promoted when acetosyringone was present, with more than 80% of color removal in 1h at pH 6.6 or 9.0. The unusual properties indicated a high potential in industrial applications for this novel spore laccase.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>21868217</pmid><doi>10.1016/j.biortech.2011.07.111</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-8524 |
| ispartof | Bioresource technology, 2012-07, Vol.115, p.35-40 |
| issn | 0960-8524 1873-2976 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1770374768 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adaptation, Physiological - drug effects Alkalies - pharmacology Bacillus - drug effects Bacillus - enzymology Bacillus - genetics Bacillus - isolation & purification Bacillus licheniformis Bacteria Base Sequence Biodegradation, Environmental - drug effects Characterization Color Coloring Agents - isolation & purification Decoloring Decolorization Enzyme Inhibitors - pharmacology Enzyme Stability - drug effects Hydrogen-Ion Concentration - drug effects Isolation Laccase Laccase - metabolism Organic Chemicals - pharmacology Phylogeny RNA, Ribosomal, 16S - genetics Solvents Solvents - pharmacology Spores Spores, Bacterial - enzymology Substrate Specificity - drug effects Temperature |
| title | Characterization and dye decolorization ability of an alkaline resistant and organic solvents tolerant laccase from Bacillus licheniformis LS04 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T09%3A53%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20and%20dye%20decolorization%20ability%20of%20an%20alkaline%20resistant%20and%20organic%20solvents%20tolerant%20laccase%20from%20Bacillus%20licheniformis%20LS04&rft.jtitle=Bioresource%20technology&rft.au=Lu,%20Lei&rft.date=2012-07&rft.volume=115&rft.spage=35&rft.epage=40&rft.pages=35-40&rft.issn=0960-8524&rft.eissn=1873-2976&rft_id=info:doi/10.1016/j.biortech.2011.07.111&rft_dat=%3Cproquest_cross%3E1038617591%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1017763032&rft_id=info:pmid/21868217&rft_els_id=S0960852411010790&rfr_iscdi=true |