Single-Digit Pathogen and Attomolar Detection with the Naked Eye Using Liposome-Amplified Plasmonic Immunoassay
We introduce an enzyme-free plasmonic immunoassay with a binary (all-or-none) response. The presence of a single pathogen in the sample results in a chemical cascade reaction leading to a large red to dark-blue colorimetric shift visible to the naked eye. The immediate and amplified response is init...
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| Veröffentlicht in: | Nano letters 2015-09, Vol.15 (9), p.6239-6246 |
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| container_end_page | 6246 |
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| container_issue | 9 |
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| container_title | Nano letters |
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| creator | Bui, Minh-Phuong Ngoc Ahmed, Snober Abbas, Abdennour |
| description | We introduce an enzyme-free plasmonic immunoassay with a binary (all-or-none) response. The presence of a single pathogen in the sample results in a chemical cascade reaction leading to a large red to dark-blue colorimetric shift visible to the naked eye. The immediate and amplified response is initiated by a triggered breakdown of cysteine-loaded nanoliposomes and subsequent aggregation of plasmonic gold nanoparticles. Our approach enabled visual detection of a single-digit live pathogen of Salmonella, Listeria, and E. coli O157 in water and food samples. Furthermore, the assay allowed a naked-eye detection of target antibody concentrations as low as 6.7 attomolar (600 molecules in 150 μL); six orders of magnitude lower than conventional enzyme-linked immunosorbent assay (ELISA). |
| doi_str_mv | 10.1021/acs.nanolett.5b02837 |
| format | Article |
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The presence of a single pathogen in the sample results in a chemical cascade reaction leading to a large red to dark-blue colorimetric shift visible to the naked eye. The immediate and amplified response is initiated by a triggered breakdown of cysteine-loaded nanoliposomes and subsequent aggregation of plasmonic gold nanoparticles. Our approach enabled visual detection of a single-digit live pathogen of Salmonella, Listeria, and E. coli O157 in water and food samples. Furthermore, the assay allowed a naked-eye detection of target antibody concentrations as low as 6.7 attomolar (600 molecules in 150 μL); six orders of magnitude lower than conventional enzyme-linked immunosorbent assay (ELISA).</description><subject>Amplification</subject><subject>Animals</subject><subject>Assaying</subject><subject>Colorimetry</subject><subject>Colorimetry - methods</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>Food Microbiology</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Immunoassay - methods</subject><subject>Liposomes - chemistry</subject><subject>Liposomes - ultrastructure</subject><subject>Listeria - isolation & purification</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - ultrastructure</subject><subject>Nanostructure</subject><subject>Pathogens</subject><subject>Plasmonics</subject><subject>Rabbits</subject><subject>Salmonella - isolation & purification</subject><subject>Salmonella Infections - microbiology</subject><subject>Water Microbiology</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1LxDAQhoMofv8DkRy9dJ0kTZseF79hUUE9l7Sd7kabZG1SZP-9XXb1KJ4ykOd9B-Yh5IzBhAFnl7oOE6ed7zDGiayAK5HvkEMmBSRZUfDd31mlB-QohHcAKISEfXLAMwFKqPyQ-Bfj5h0m12ZuIn3WceHn6Kh2DZ3G6K3vdE-vMWIdjXf0y8QFjQukj_oDG3qzQvoWxgY6M0sfvMVkapedac34-dzpYL0zNX2wdnBeh6BXJ2Sv1V3A0-17TN5ub16v7pPZ093D1XSWaKFYTFKOeYaQNlJVKUMpVYtcKS45FJBhIxTUTHBVSZBpUSFjjKeFYJXQqhUcxDG52PQue_85YIilNaHGrtMO_RBKlucgRCYL9g-UQaGkzNdoukHr3ofQY1sue2N1vyoZlGsr5Wil_LFSbq2MsfPthqGy2PyGfjSMAGyAdfzdD70bb_N35zeIxpuX</recordid><startdate>20150909</startdate><enddate>20150909</enddate><creator>Bui, Minh-Phuong Ngoc</creator><creator>Ahmed, Snober</creator><creator>Abbas, Abdennour</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20150909</creationdate><title>Single-Digit Pathogen and Attomolar Detection with the Naked Eye Using Liposome-Amplified Plasmonic Immunoassay</title><author>Bui, Minh-Phuong Ngoc ; 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| fulltext | fulltext |
| identifier | ISSN: 1530-6984 |
| ispartof | Nano letters, 2015-09, Vol.15 (9), p.6239-6246 |
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| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Amplification Animals Assaying Colorimetry Colorimetry - methods Enzyme-Linked Immunosorbent Assay Escherichia coli Infections - microbiology Escherichia coli O157 - isolation & purification Food Microbiology Gold Gold - chemistry Humans Immunoassay Immunoassay - methods Liposomes - chemistry Liposomes - ultrastructure Listeria - isolation & purification Metal Nanoparticles - chemistry Metal Nanoparticles - ultrastructure Nanostructure Pathogens Plasmonics Rabbits Salmonella - isolation & purification Salmonella Infections - microbiology Water Microbiology |
| title | Single-Digit Pathogen and Attomolar Detection with the Naked Eye Using Liposome-Amplified Plasmonic Immunoassay |
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