Integrated label-free silicon nanowire sensor arrays for (bio)chemical analysis
We present a label-free (bio)chemical analysis platform that uses all-electrical silicon nanowire sensor arrays integrated with a small volume microfluidic flow-cell for real-time (bio)chemical analysis and detection. The integrated sensing platform contains an automated multi-sample injection syste...
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| Veröffentlicht in: | Analyst (London) 2013-06, Vol.138 (11), p.3221-3229 |
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| container_title | Analyst (London) |
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| creator | De, Arpita van Nieuwkasteele, Jan Carlen, Edwin T van den Berg, Albert |
| description | We present a label-free (bio)chemical analysis platform that uses all-electrical silicon nanowire sensor arrays integrated with a small volume microfluidic flow-cell for real-time (bio)chemical analysis and detection. The integrated sensing platform contains an automated multi-sample injection system that eliminates erroneous sensor responses from sample switching due to flow rate fluctuations and provides precise sample volumes down to 10 nl. Biochemical sensing is demonstrated with real-time 15-mer DNA-PNA (peptide nucleic acid) duplex hybridization measurements from different sample concentrations in a low ionic strength, and the equilibrium dissociation constant KD ≈ 140 nM has been extracted from the experimental data using the first order Langmuir binding model. Chemical sensing is demonstrated with pH measurements from different injected samples in flow that have sensitivities consistent with the gate-oxide materials. A differential sensor measurement configuration results in a 30× reduction in sensor drift. The integrated label-free analysis platform is suitable for a wide range of small volume chemical and biochemical analyses. |
| doi_str_mv | 10.1039/c3an36586g |
| format | Article |
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| ispartof | Analyst (London), 2013-06, Vol.138 (11), p.3221-3229 |
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| language | eng |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Biochemistry Biosensing Techniques - instrumentation Buffers Detection DNA - chemistry Hydrogen-Ion Concentration Microfluidic Analytical Techniques - instrumentation Nanomaterials Nanostructure Nanotechnology - instrumentation Nanowires Nucleic Acid Hybridization Peptide Nucleic Acids - chemistry Platforms Real time Sensor arrays Sensors Silicon - chemistry Systems Integration Transistors, Electronic |
| title | Integrated label-free silicon nanowire sensor arrays for (bio)chemical analysis |
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