Pichia pastoris production of a prolyl 4-hydroxylase derived from Chondrosia reniformis sponge: A new biotechnological tool for the recombinant production of marine collagen

•Production of yeast strain expressing an active sponge P4H tetramer.•Production of yeast strain hydroxylating a procollagen polypeptide from C. reniformis.•Biotechnological tool hydroxylating prolines in X and Y position in marine collagens. Prolyl 4-hydroxylase (P4H) is a α2β2 tetramer catalyzing...

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