Development of ELISA and colloidal gold immunoassay for tetrodotoxin detetcion based on monoclonal antibody

A monoclonal hybridoma cell named 5B9 against tetrodotoxin (TTX) was obtained after fusion of myeloma SP2/0 cells with spleen cells isolated from the immunized Balb/c mice. The 5B9 monoclonal antibody (McAb) with high affinity (about 2.55×109) is specific to TTX, and this McAb belongs to the immunog...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biosensors & bioelectronics 2015-09, Vol.71, p.256-260
Hauptverfasser:	Ling, Sumei, Chen, Qing-Ai, Zhang, Yuming, Wang, Rongzhi, Jin, Ni, Pang, Jie, Wang, Shihua
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Colloidal gold immunoassay Colloids ELISA Enzyme-Linked Immunosorbent Assay - methods Female Gold Gold Colloid - chemistry Hybridomas - immunology Immunoassay Limit of Detection Marine Toxins - analysis Marine Toxins - immunology Mice, Inbred BALB C Monoclonal antibodies Monoclonal antibody Strip Tetraodontiformes Tetrodotoxin Tetrodotoxin - analysis Tetrodotoxin - immunology TTX
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!