Microfluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA
Some of the cyanobacteria produce protease inhibitor oligopeptides such as cyanopeptolins and cause drinking water contamination; hence, their detection has great importance to monitor the well-being of water sources that is used for human consumption. In the current study, a fast and sensitive nucl...
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creator | Ölcer, Zehra Esen, Elif Ersoy, Aylin Budak, Sinan Sever Kaya, Dilek Yağmur Gök, Mehmet Barut, Serkan Üstek, Duran Uludag, Yildiz |
description | Some of the cyanobacteria produce protease inhibitor oligopeptides such as cyanopeptolins and cause drinking water contamination; hence, their detection has great importance to monitor the well-being of water sources that is used for human consumption. In the current study, a fast and sensitive nucleic acid biosensor assay has been described where cyanopeptolin coding region of one of the cyanobacteria (Planktothrix agardhii NIVA-CYA 116) genome has been used as target for monitoring of the fresh water resources. A biochip that has two sets of Au electrode arrays, each consist of shared reference/counter electrodes and 3 working electrodes has been used for the assay. The biochip has been integrated to a microfluidics system and all steps of the assay have been performed during the reagent flow to achieve fast and sensitive DNA detection. On-line hybridization of the target on to the capture probe immobilized surface resulted in a very short assay duration with respect to the conventional static assays. The binding of the avidin and enzyme modified Au nanoparticles to the biotinylated detection probe and the subsequent injection of the substrate enabled a real-time amperometric measurement with a detection limit of 6×10−12M target DNA (calibration curve r2=0.98). The developed assay enables fast and sensitive detection of cyanopeptolin producing cyanobacteria from freshwater samples and hence shows a promising technology for toxic microorganism detection from environmental samples.
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•Electrode array integrated microfluidics fabricated and used.•Real-time amperometric detection achieved with non-stop microfluidic flow.•Cyanobacteria detection achieved down to picomolar.•PCR products tested successfully. |
doi_str_mv | 10.1016/j.bios.2015.03.052 |
format | Article |
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[Display omitted]
•Electrode array integrated microfluidics fabricated and used.•Real-time amperometric detection achieved with non-stop microfluidic flow.•Cyanobacteria detection achieved down to picomolar.•PCR products tested successfully.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2015.03.052</identifier><identifier>PMID: 25845335</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amperometry ; Assaying ; Biosensing Techniques - instrumentation ; Biosensors ; Conductometry - instrumentation ; Cyanobacteria ; Cyanobacteria - genetics ; Cyanobacteria - isolation & purification ; Cyanopeptolin ; Deoxyribonucleic acid ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; Drinking water ; Electrical measurements ; Electrochemical sensing ; Electrodes ; Equipment Design ; Equipment Failure Analysis ; Gold - chemistry ; Lab-On-A-Chip Devices ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - ultrastructure ; Microelectrodes ; Microfluidics ; Planktothrix agardhii ; Real-time electrochemical profiling ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Biosensors & bioelectronics, 2015-08, Vol.70, p.426-432</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-8577be3af143974b5a1d5d3bebdafc744691f08d6d51f15821888069ac80f55a3</citedby><cites>FETCH-LOGICAL-c422t-8577be3af143974b5a1d5d3bebdafc744691f08d6d51f15821888069ac80f55a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2015.03.052$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25845335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ölcer, Zehra</creatorcontrib><creatorcontrib>Esen, Elif</creatorcontrib><creatorcontrib>Ersoy, Aylin</creatorcontrib><creatorcontrib>Budak, Sinan</creatorcontrib><creatorcontrib>Sever Kaya, Dilek</creatorcontrib><creatorcontrib>Yağmur Gök, Mehmet</creatorcontrib><creatorcontrib>Barut, Serkan</creatorcontrib><creatorcontrib>Üstek, Duran</creatorcontrib><creatorcontrib>Uludag, Yildiz</creatorcontrib><title>Microfluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Some of the cyanobacteria produce protease inhibitor oligopeptides such as cyanopeptolins and cause drinking water contamination; hence, their detection has great importance to monitor the well-being of water sources that is used for human consumption. In the current study, a fast and sensitive nucleic acid biosensor assay has been described where cyanopeptolin coding region of one of the cyanobacteria (Planktothrix agardhii NIVA-CYA 116) genome has been used as target for monitoring of the fresh water resources. A biochip that has two sets of Au electrode arrays, each consist of shared reference/counter electrodes and 3 working electrodes has been used for the assay. The biochip has been integrated to a microfluidics system and all steps of the assay have been performed during the reagent flow to achieve fast and sensitive DNA detection. On-line hybridization of the target on to the capture probe immobilized surface resulted in a very short assay duration with respect to the conventional static assays. The binding of the avidin and enzyme modified Au nanoparticles to the biotinylated detection probe and the subsequent injection of the substrate enabled a real-time amperometric measurement with a detection limit of 6×10−12M target DNA (calibration curve r2=0.98). The developed assay enables fast and sensitive detection of cyanopeptolin producing cyanobacteria from freshwater samples and hence shows a promising technology for toxic microorganism detection from environmental samples.
[Display omitted]
•Electrode array integrated microfluidics fabricated and used.•Real-time amperometric detection achieved with non-stop microfluidic flow.•Cyanobacteria detection achieved down to picomolar.•PCR products tested successfully.</description><subject>Amperometry</subject><subject>Assaying</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensors</subject><subject>Conductometry - instrumentation</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - isolation & purification</subject><subject>Cyanopeptolin</subject><subject>Deoxyribonucleic acid</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>Drinking water</subject><subject>Electrical measurements</subject><subject>Electrochemical sensing</subject><subject>Electrodes</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Gold - chemistry</subject><subject>Lab-On-A-Chip Devices</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - ultrastructure</subject><subject>Microelectrodes</subject><subject>Microfluidics</subject><subject>Planktothrix agardhii</subject><subject>Real-time electrochemical profiling</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uFDEQhC0EIkvgBTggH8NhBv-MPR6Jy2r5ixQCB0DiZHnsNutlZryxvYi8AY-NVxs4Ig6tvnxV3apC6CklLSVUvti1Y4i5ZYSKlvCWCHYPrajqedMxLu6jFRmEbISU_Aw9ynlHCOnpQB6iMyZUJzgXK_TrfbAp-ukQXLAZm8XhxSxxb1IJdoKMR5PBYTPvIcUZSgoWH6_CkmPCvk7ZAnZQwJYQFxw9trfVYDS2QAoGX3yczPK9xLJN4Sc230xy2xDw9eWXdbP5usaUyuf41fX6MXrgzZThyd0-R5_fvP60eddcfXh7uVlfNbZjrDRK9P0I3Hja8aHvRmGoE46PMDrjbd91cqCeKCedoJ4KxahSisjBWEW8EIafo4uT7z7FmwPkoueQLUz1S4iHrGnfE854N8j_QLkYKJO9qig7oTXMnBN4vU9hNulWU6KPZemdPsamj2VpwnUtq4qe3fkfxhncX8mfdirw8gRADeRHgKSzDbBYcCHVvLWL4V_-vwGJq6Xs</recordid><startdate>20150815</startdate><enddate>20150815</enddate><creator>Ölcer, Zehra</creator><creator>Esen, Elif</creator><creator>Ersoy, Aylin</creator><creator>Budak, Sinan</creator><creator>Sever Kaya, Dilek</creator><creator>Yağmur Gök, Mehmet</creator><creator>Barut, Serkan</creator><creator>Üstek, Duran</creator><creator>Uludag, Yildiz</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7SP</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>20150815</creationdate><title>Microfluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA</title><author>Ölcer, Zehra ; Esen, Elif ; Ersoy, Aylin ; Budak, Sinan ; Sever Kaya, Dilek ; Yağmur Gök, Mehmet ; Barut, Serkan ; Üstek, Duran ; Uludag, Yildiz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-8577be3af143974b5a1d5d3bebdafc744691f08d6d51f15821888069ac80f55a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amperometry</topic><topic>Assaying</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensors</topic><topic>Conductometry - instrumentation</topic><topic>Cyanobacteria</topic><topic>Cyanobacteria - genetics</topic><topic>Cyanobacteria - isolation & purification</topic><topic>Cyanopeptolin</topic><topic>Deoxyribonucleic acid</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>Drinking water</topic><topic>Electrical measurements</topic><topic>Electrochemical sensing</topic><topic>Electrodes</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Gold - chemistry</topic><topic>Lab-On-A-Chip Devices</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metal Nanoparticles - ultrastructure</topic><topic>Microelectrodes</topic><topic>Microfluidics</topic><topic>Planktothrix agardhii</topic><topic>Real-time electrochemical profiling</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ölcer, Zehra</creatorcontrib><creatorcontrib>Esen, Elif</creatorcontrib><creatorcontrib>Ersoy, Aylin</creatorcontrib><creatorcontrib>Budak, Sinan</creatorcontrib><creatorcontrib>Sever Kaya, Dilek</creatorcontrib><creatorcontrib>Yağmur Gök, Mehmet</creatorcontrib><creatorcontrib>Barut, Serkan</creatorcontrib><creatorcontrib>Üstek, Duran</creatorcontrib><creatorcontrib>Uludag, Yildiz</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ölcer, Zehra</au><au>Esen, Elif</au><au>Ersoy, Aylin</au><au>Budak, Sinan</au><au>Sever Kaya, Dilek</au><au>Yağmur Gök, Mehmet</au><au>Barut, Serkan</au><au>Üstek, Duran</au><au>Uludag, Yildiz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microfluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2015-08-15</date><risdate>2015</risdate><volume>70</volume><spage>426</spage><epage>432</epage><pages>426-432</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Some of the cyanobacteria produce protease inhibitor oligopeptides such as cyanopeptolins and cause drinking water contamination; hence, their detection has great importance to monitor the well-being of water sources that is used for human consumption. In the current study, a fast and sensitive nucleic acid biosensor assay has been described where cyanopeptolin coding region of one of the cyanobacteria (Planktothrix agardhii NIVA-CYA 116) genome has been used as target for monitoring of the fresh water resources. A biochip that has two sets of Au electrode arrays, each consist of shared reference/counter electrodes and 3 working electrodes has been used for the assay. The biochip has been integrated to a microfluidics system and all steps of the assay have been performed during the reagent flow to achieve fast and sensitive DNA detection. On-line hybridization of the target on to the capture probe immobilized surface resulted in a very short assay duration with respect to the conventional static assays. The binding of the avidin and enzyme modified Au nanoparticles to the biotinylated detection probe and the subsequent injection of the substrate enabled a real-time amperometric measurement with a detection limit of 6×10−12M target DNA (calibration curve r2=0.98). The developed assay enables fast and sensitive detection of cyanopeptolin producing cyanobacteria from freshwater samples and hence shows a promising technology for toxic microorganism detection from environmental samples.
[Display omitted]
•Electrode array integrated microfluidics fabricated and used.•Real-time amperometric detection achieved with non-stop microfluidic flow.•Cyanobacteria detection achieved down to picomolar.•PCR products tested successfully.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>25845335</pmid><doi>10.1016/j.bios.2015.03.052</doi><tpages>7</tpages></addata></record> |
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subjects | Amperometry Assaying Biosensing Techniques - instrumentation Biosensors Conductometry - instrumentation Cyanobacteria Cyanobacteria - genetics Cyanobacteria - isolation & purification Cyanopeptolin Deoxyribonucleic acid DNA, Bacterial - analysis DNA, Bacterial - genetics Drinking water Electrical measurements Electrochemical sensing Electrodes Equipment Design Equipment Failure Analysis Gold - chemistry Lab-On-A-Chip Devices Metal Nanoparticles - chemistry Metal Nanoparticles - ultrastructure Microelectrodes Microfluidics Planktothrix agardhii Real-time electrochemical profiling Reproducibility of Results Sensitivity and Specificity |
title | Microfluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA |
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