High-yield production of a functional bacteriophage lysin with antipneumococcal activity using a plant virus-based expression system

•S. pneumoniae phage lysin Pal was produced in a plant virus-based expression system.•Highly pure Pal was obtained after purification by DEAE sepharose.•Plant-produced Pal was active against three tested S. pneumoniae strains.•Pal treatment of bacteremic mice demonstrated a decrease in blood bacteri...

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Veröffentlicht in:	Journal of biotechnology 2015-04, Vol.200, p.10-16
Hauptverfasser:	Starkevič, Urtė, Bortesi, Luisa, Virgailis, Marius, Ružauskas, Modestas, Giritch, Anatoli, Ražanskienė, Aušra
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens - genetics Amidohydrolases - genetics Amidohydrolases - metabolism Amidohydrolases - pharmacology Amidohydrolases - therapeutic use Animals Anti-Bacterial Agents - metabolism Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Antibiotics Antiinfectives and antibacterials Bacteremia - drug therapy Bacteria Bacteriophage lysin Bacteriophages Enzymes In vitro testing Mice Mice bacteremia and survival Mice, Inbred BALB C Mucoproteins Nicotiana - physiology Nicotiana - virology Nicotiana benthamiana Plant Leaves - physiology Plant Leaves - virology Plant virus-based transient expression Strain Streptococcus Phages Streptococcus pneumoniae Streptococcus pneumoniae - drug effects Streptococcus pneumoniae - physiology Tobacco mosaic virus Tobacco Mosaic Virus - physiology Viral Proteins - genetics Viral Proteins - metabolism Viral Proteins - pharmacology Viral Proteins - therapeutic use
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