Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine
Quantitative cysteine-independent ligation of a Gd(3+) tag to genetically encoded p-azido-L-phenylalanine via Cu(I)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd(3+)-Gd(3+) distance measurements by double electron-electron resonance (DEER) experiments, as the po...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2015-01, Vol.51 (88), p.15898-15901 |
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| container_issue | 88 |
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| container_title | Chemical communications (Cambridge, England) |
| container_volume | 51 |
| creator | Abdelkader, E H Feintuch, A Yao, X Adams, L A Aurelio, L Graham, B Goldfarb, D Otting, G |
| description | Quantitative cysteine-independent ligation of a Gd(3+) tag to genetically encoded p-azido-L-phenylalanine via Cu(I)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd(3+)-Gd(3+) distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd(3+) ion relative to the protein can be predicted with high accuracy. |
| doi_str_mv | 10.1039/c5cc07121f |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2015-01, Vol.51 (88), p.15898-15901 |
| issn | 1359-7345 1364-548X |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Azides - chemistry Carrier Proteins - chemical synthesis Carrier Proteins - chemistry Click Chemistry Deer Electron Spin Resonance Spectroscopy Escherichia coli - chemistry Gadolinium Glutamates - chemistry Mutagenesis, Site-Directed Phenylalanine - analogs & derivatives Phenylalanine - chemistry Phenylalanine - genetics Position measurement Protein Structure, Tertiary Proteins Spectroscopy Spin Labels Synthesis (chemistry) Tags |
| title | Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine |
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