Label-free electrochemical detection of prostate-specific antigen based on nucleic acid aptamer
We report a label-free aptasensor to make direct detection of prostate specific antigen (PSA, a biomarker of prostate cancer) using a quinone-containing conducting copolymer acting as redox transducer and grafting matrix for immobilization of the short aptamer strands. It is shown that capture of PS...
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| Veröffentlicht in: | Biosensors & bioelectronics 2015-06, Vol.68, p.49-54 |
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| container_title | Biosensors & bioelectronics |
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| creator | Souada, M Piro, B Reisberg, S Anquetin, G Noël, V Pham, M C |
| description | We report a label-free aptasensor to make direct detection of prostate specific antigen (PSA, a biomarker of prostate cancer) using a quinone-containing conducting copolymer acting as redox transducer and grafting matrix for immobilization of the short aptamer strands. It is shown that capture of PSA generates a current decrease (signal-off) measured by Square Wave Voltammetry. This current decrease is specific for PSA above a limit of quantification in the ng mL(-1) range. The change in current is used to determine the PSA-aptamer dissociation constant K(D), of ca. 2.6 nM. To consolidate the proof of concept, a heterogeneous competitive exchange with a complementary DNA strand which breaks PSA-aptamer interactions is studied. This double-check followed by a current increase provides full assurance of a perfectly specific recognition. |
| doi_str_mv | 10.1016/j.bios.2014.12.033 |
| format | Article |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Antigens Aptamers, Nucleotide - chemistry Biosensing Techniques Biosensors Constants Exchange Humans Male Oligonucleotides - chemistry Prostate Prostate-Specific Antigen - chemistry Prostate-Specific Antigen - isolation & purification Prostatic Neoplasms - diagnosis Recognition Strands Transducers |
| title | Label-free electrochemical detection of prostate-specific antigen based on nucleic acid aptamer |
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