Ultrastructural changes of kidney in diabetic rats
To demonstrate histopathological changes in diabetic kidneys of the rat on ultrastructural level with focusing on glomerular cellular environment. Male Wistar rats were administered streptozotocin and after 8 weeks, their kidneys were studied using qualitative transmission electron microscope analys...
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| Veröffentlicht in: | Bratislava Medical Journal 2016, Vol.117 (3), p.161-165 |
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| creator | Miko, M Jakubovsky, J Vrabcova, M Varga, I |
| description | To demonstrate histopathological changes in diabetic kidneys of the rat on ultrastructural level with focusing on glomerular cellular environment.
Male Wistar rats were administered streptozotocin and after 8 weeks, their kidneys were studied using qualitative transmission electron microscope analysis.
In diabetic kidneys, the presence of irregularly thickened glomerular membrana basalis (GBM), often up to six times as compared to healthy kidney GBM, mainly around peripheral capillary loops, was striking. We also demonstrated lamination and split appearance of GBM, with presence of delicate fibrillar structure, which was absent within normal GBM. Accumulation of mesangial matrix was found only occasionally.
Model may be useful to better understand the mechanisms that play role in the progression of DM-induced renal disease and to comprehend the morphological changes, which this process leaves within the tissue. The clear association between ultra-structure and functional markers may not be necessary (Fig. 4, Ref. 27). |
| doi_str_mv | 10.4149/BLL_2016_029 |
| format | Article |
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Male Wistar rats were administered streptozotocin and after 8 weeks, their kidneys were studied using qualitative transmission electron microscope analysis.
In diabetic kidneys, the presence of irregularly thickened glomerular membrana basalis (GBM), often up to six times as compared to healthy kidney GBM, mainly around peripheral capillary loops, was striking. We also demonstrated lamination and split appearance of GBM, with presence of delicate fibrillar structure, which was absent within normal GBM. Accumulation of mesangial matrix was found only occasionally.
Model may be useful to better understand the mechanisms that play role in the progression of DM-induced renal disease and to comprehend the morphological changes, which this process leaves within the tissue. The clear association between ultra-structure and functional markers may not be necessary (Fig. 4, Ref. 27).</description><identifier>ISSN: 0006-9248</identifier><identifier>ISSN: 1336-0345</identifier><identifier>EISSN: 1336-0345</identifier><identifier>DOI: 10.4149/BLL_2016_029</identifier><identifier>PMID: 26925747</identifier><language>eng</language><publisher>Slovakia</publisher><subject>Animals ; Basement Membrane ; Diabetes Mellitus, Experimental - complications ; Diabetes Mellitus, Experimental - pathology ; Diabetic Nephropathies - pathology ; Disease Progression ; Extracellular Matrix ; Kidney - pathology ; Kidney - ultrastructure ; Kidney Glomerulus ; Male ; Microscopy, Electron, Transmission ; Rats ; Rats, Wistar ; Streptozocin</subject><ispartof>Bratislava Medical Journal, 2016, Vol.117 (3), p.161-165</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-753effc8e181f6c2cd1013d447c04c3a4eccb88c87d6985a056132b633fb2e0d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26925747$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miko, M</creatorcontrib><creatorcontrib>Jakubovsky, J</creatorcontrib><creatorcontrib>Vrabcova, M</creatorcontrib><creatorcontrib>Varga, I</creatorcontrib><title>Ultrastructural changes of kidney in diabetic rats</title><title>Bratislava Medical Journal</title><addtitle>Bratisl Lek Listy</addtitle><description>To demonstrate histopathological changes in diabetic kidneys of the rat on ultrastructural level with focusing on glomerular cellular environment.
Male Wistar rats were administered streptozotocin and after 8 weeks, their kidneys were studied using qualitative transmission electron microscope analysis.
In diabetic kidneys, the presence of irregularly thickened glomerular membrana basalis (GBM), often up to six times as compared to healthy kidney GBM, mainly around peripheral capillary loops, was striking. We also demonstrated lamination and split appearance of GBM, with presence of delicate fibrillar structure, which was absent within normal GBM. Accumulation of mesangial matrix was found only occasionally.
Model may be useful to better understand the mechanisms that play role in the progression of DM-induced renal disease and to comprehend the morphological changes, which this process leaves within the tissue. The clear association between ultra-structure and functional markers may not be necessary (Fig. 4, Ref. 27).</description><subject>Animals</subject><subject>Basement Membrane</subject><subject>Diabetes Mellitus, Experimental - complications</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Diabetic Nephropathies - pathology</subject><subject>Disease Progression</subject><subject>Extracellular Matrix</subject><subject>Kidney - pathology</subject><subject>Kidney - ultrastructure</subject><subject>Kidney Glomerulus</subject><subject>Male</subject><subject>Microscopy, Electron, Transmission</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Streptozocin</subject><issn>0006-9248</issn><issn>1336-0345</issn><issn>1336-0345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0DtPwzAYhWELgWgpbMwoIwOBz9fYI1TcpEgsdLYcXyCQS7Gdof-eohbEdJZHZ3gROsdwzTBTN3d1rQlgoYGoAzTHlIoSKOOHaA4AolSEyRk6SekDgFGOxTGaEaEIr1g1R2TV5WhSjpPNUzRdYd_N8OZTMYbis3WD3xTtULjWND63togmp1N0FEyX_Nl-F2j1cP-6fCrrl8fn5W1dWqpULitOfQhWeixxEJZYhwFTx1hlgVlqmLe2kdLKygkluQEuMCWNoDQ0xIOjC3S5-13H8WvyKeu-TdZ3nRn8OCWNqwoIoULxLb3aURvHlKIPeh3b3sSNxqB_Kun_lbb8Yv88Nb13f_g3C_0GOz5hrA</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Miko, M</creator><creator>Jakubovsky, J</creator><creator>Vrabcova, M</creator><creator>Varga, I</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2016</creationdate><title>Ultrastructural changes of kidney in diabetic rats</title><author>Miko, M ; Jakubovsky, J ; Vrabcova, M ; Varga, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-753effc8e181f6c2cd1013d447c04c3a4eccb88c87d6985a056132b633fb2e0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Basement Membrane</topic><topic>Diabetes Mellitus, Experimental - complications</topic><topic>Diabetes Mellitus, Experimental - pathology</topic><topic>Diabetic Nephropathies - pathology</topic><topic>Disease Progression</topic><topic>Extracellular Matrix</topic><topic>Kidney - pathology</topic><topic>Kidney - ultrastructure</topic><topic>Kidney Glomerulus</topic><topic>Male</topic><topic>Microscopy, Electron, Transmission</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Streptozocin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miko, M</creatorcontrib><creatorcontrib>Jakubovsky, J</creatorcontrib><creatorcontrib>Vrabcova, M</creatorcontrib><creatorcontrib>Varga, I</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bratislava Medical Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miko, M</au><au>Jakubovsky, J</au><au>Vrabcova, M</au><au>Varga, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructural changes of kidney in diabetic rats</atitle><jtitle>Bratislava Medical Journal</jtitle><addtitle>Bratisl Lek Listy</addtitle><date>2016</date><risdate>2016</risdate><volume>117</volume><issue>3</issue><spage>161</spage><epage>165</epage><pages>161-165</pages><issn>0006-9248</issn><issn>1336-0345</issn><eissn>1336-0345</eissn><abstract>To demonstrate histopathological changes in diabetic kidneys of the rat on ultrastructural level with focusing on glomerular cellular environment.
Male Wistar rats were administered streptozotocin and after 8 weeks, their kidneys were studied using qualitative transmission electron microscope analysis.
In diabetic kidneys, the presence of irregularly thickened glomerular membrana basalis (GBM), often up to six times as compared to healthy kidney GBM, mainly around peripheral capillary loops, was striking. We also demonstrated lamination and split appearance of GBM, with presence of delicate fibrillar structure, which was absent within normal GBM. Accumulation of mesangial matrix was found only occasionally.
Model may be useful to better understand the mechanisms that play role in the progression of DM-induced renal disease and to comprehend the morphological changes, which this process leaves within the tissue. The clear association between ultra-structure and functional markers may not be necessary (Fig. 4, Ref. 27).</abstract><cop>Slovakia</cop><pmid>26925747</pmid><doi>10.4149/BLL_2016_029</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Bratislava Medical Journal, 2016, Vol.117 (3), p.161-165 |
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| subjects | Animals Basement Membrane Diabetes Mellitus, Experimental - complications Diabetes Mellitus, Experimental - pathology Diabetic Nephropathies - pathology Disease Progression Extracellular Matrix Kidney - pathology Kidney - ultrastructure Kidney Glomerulus Male Microscopy, Electron, Transmission Rats Rats, Wistar Streptozocin |
| title | Ultrastructural changes of kidney in diabetic rats |
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