The investigation of the prenatal and postnatal alcohol exposure-induced neurodegeneration in rat brain: protection by betaine and/or omega-3
Purpose We aim to study the effect of neurodegeneration on the brain of rat pups caused by prenatal and postnatal ethanol exposure with modified liquid diet to elucidate protective effects of betaine and omega-3 supplementation. When ethanol is consumed during prenatal and postnatal periods, it may...
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| Veröffentlicht in: | Child's nervous system 2016-03, Vol.32 (3), p.467-474 |
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| creator | Kusat Ol, Kevser Kanbak, Güngör Oğlakcı Ilhan, Ayşegül Burukoglu, Dilek Yücel, Ferruh |
| description | Purpose
We aim to study the effect of neurodegeneration on the brain of rat pups caused by prenatal and postnatal ethanol exposure with modified liquid diet to elucidate protective effects of betaine and omega-3 supplementation. When ethanol is consumed during prenatal and postnatal periods, it may result in fetal alcohol syndrome (FAS) in the offspring.
Methods
Rats were divided into control, ethanol, ethanol + betaine, ethanol + omega-3, ethanol + omega-3 + betaine groups. The effect of betaine and omega-3 in response to ethanol-induced changes on the brain, by biochemical analyses cytochrome c, caspase-3, calpain, cathepsin B and L, DNA fragmentation, histological and morfometric methods were evaluated.
Results
Caspase-3, calpain, cathepsin B, and cytochrome c levels in ethanol group were significantly higher than control. Caspase-3, calpain levels were decreased in ethanol + betaine, ethanol + omega-3, and ethanol + omega-3 + betaine groups compared to ethanol group. Cathepsin B in ethanol + omega-3 + betaine group was decreased compared to ethanol, ethanol + betaine groups. Cathepsin L and DNA fragmentation were found not statistically significant. We found similar results in histological and morfometric parameters.
Conclusion
We found that pre- and postnatal ethanol exposure is capable of triggering necrotic cell death in rat brains, omega-3, and betaine reduce neurodegeneration. Omega-3 and betaine may prove beneficial for neurodegeneration, particularly in preventing FAS. |
| doi_str_mv | 10.1007/s00381-015-2990-1 |
| format | Article |
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We aim to study the effect of neurodegeneration on the brain of rat pups caused by prenatal and postnatal ethanol exposure with modified liquid diet to elucidate protective effects of betaine and omega-3 supplementation. When ethanol is consumed during prenatal and postnatal periods, it may result in fetal alcohol syndrome (FAS) in the offspring.
Methods
Rats were divided into control, ethanol, ethanol + betaine, ethanol + omega-3, ethanol + omega-3 + betaine groups. The effect of betaine and omega-3 in response to ethanol-induced changes on the brain, by biochemical analyses cytochrome c, caspase-3, calpain, cathepsin B and L, DNA fragmentation, histological and morfometric methods were evaluated.
Results
Caspase-3, calpain, cathepsin B, and cytochrome c levels in ethanol group were significantly higher than control. Caspase-3, calpain levels were decreased in ethanol + betaine, ethanol + omega-3, and ethanol + omega-3 + betaine groups compared to ethanol group. Cathepsin B in ethanol + omega-3 + betaine group was decreased compared to ethanol, ethanol + betaine groups. Cathepsin L and DNA fragmentation were found not statistically significant. We found similar results in histological and morfometric parameters.
Conclusion
We found that pre- and postnatal ethanol exposure is capable of triggering necrotic cell death in rat brains, omega-3, and betaine reduce neurodegeneration. Omega-3 and betaine may prove beneficial for neurodegeneration, particularly in preventing FAS.</description><identifier>ISSN: 0256-7040</identifier><identifier>EISSN: 1433-0350</identifier><identifier>DOI: 10.1007/s00381-015-2990-1</identifier><identifier>PMID: 26732065</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Betaine - pharmacology ; Brain - drug effects ; Central Nervous System Depressants - toxicity ; Disease Models, Animal ; Ethanol - toxicity ; Fatty Acids, Omega-3 - pharmacology ; Female ; Lipotropic Agents - pharmacology ; Medicine ; Medicine & Public Health ; Nerve Degeneration - chemically induced ; Nerve Degeneration - prevention & control ; Neurosciences ; Neurosurgery ; Original Paper ; Pregnancy ; Prenatal Exposure Delayed Effects - chemically induced ; Prenatal Exposure Delayed Effects - prevention & control ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Child's nervous system, 2016-03, Vol.32 (3), p.467-474</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-2b0b31640e0ec3269c340872e4988d5cea33bf3f5eb46cdc6c9f58f0b473a7b53</citedby><cites>FETCH-LOGICAL-c414t-2b0b31640e0ec3269c340872e4988d5cea33bf3f5eb46cdc6c9f58f0b473a7b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00381-015-2990-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00381-015-2990-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26732065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kusat Ol, Kevser</creatorcontrib><creatorcontrib>Kanbak, Güngör</creatorcontrib><creatorcontrib>Oğlakcı Ilhan, Ayşegül</creatorcontrib><creatorcontrib>Burukoglu, Dilek</creatorcontrib><creatorcontrib>Yücel, Ferruh</creatorcontrib><title>The investigation of the prenatal and postnatal alcohol exposure-induced neurodegeneration in rat brain: protection by betaine and/or omega-3</title><title>Child's nervous system</title><addtitle>Childs Nerv Syst</addtitle><addtitle>Childs Nerv Syst</addtitle><description>Purpose
We aim to study the effect of neurodegeneration on the brain of rat pups caused by prenatal and postnatal ethanol exposure with modified liquid diet to elucidate protective effects of betaine and omega-3 supplementation. When ethanol is consumed during prenatal and postnatal periods, it may result in fetal alcohol syndrome (FAS) in the offspring.
Methods
Rats were divided into control, ethanol, ethanol + betaine, ethanol + omega-3, ethanol + omega-3 + betaine groups. The effect of betaine and omega-3 in response to ethanol-induced changes on the brain, by biochemical analyses cytochrome c, caspase-3, calpain, cathepsin B and L, DNA fragmentation, histological and morfometric methods were evaluated.
Results
Caspase-3, calpain, cathepsin B, and cytochrome c levels in ethanol group were significantly higher than control. Caspase-3, calpain levels were decreased in ethanol + betaine, ethanol + omega-3, and ethanol + omega-3 + betaine groups compared to ethanol group. Cathepsin B in ethanol + omega-3 + betaine group was decreased compared to ethanol, ethanol + betaine groups. Cathepsin L and DNA fragmentation were found not statistically significant. We found similar results in histological and morfometric parameters.
Conclusion
We found that pre- and postnatal ethanol exposure is capable of triggering necrotic cell death in rat brains, omega-3, and betaine reduce neurodegeneration. Omega-3 and betaine may prove beneficial for neurodegeneration, particularly in preventing FAS.</description><subject>Animals</subject><subject>Betaine - pharmacology</subject><subject>Brain - drug effects</subject><subject>Central Nervous System Depressants - toxicity</subject><subject>Disease Models, Animal</subject><subject>Ethanol - toxicity</subject><subject>Fatty Acids, Omega-3 - pharmacology</subject><subject>Female</subject><subject>Lipotropic Agents - pharmacology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nerve Degeneration - chemically induced</subject><subject>Nerve Degeneration - prevention & control</subject><subject>Neurosciences</subject><subject>Neurosurgery</subject><subject>Original Paper</subject><subject>Pregnancy</subject><subject>Prenatal Exposure Delayed Effects - chemically induced</subject><subject>Prenatal Exposure Delayed Effects - prevention & control</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0256-7040</issn><issn>1433-0350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9O3DAQxi0Egu3CA_SCfOTiMv6TOOmtQtBWQuplOVu2M1mCsvZiJ1V5iL4z3mbbY0-2v_nmJ898hHzk8IkD6NsMIBvOgFdMtC0wfkJWXEnJQFZwSlYgqpppUHBBPuT8AsXYiPacXIhaSwF1tSK_N89Ih_AT8zRs7TTEQGNPpyLuEwY72ZHa0NF9zNPxNfr4HEeKv4o2J2RD6GaPHQ04p9jhFgOmBTQEWm7UJTuEz4UXJ_R_Cu6NOpyKigf4bUw07nBrmbwkZ70dM14dzzV5erjf3H1jjz--fr_78si84mpiwoGTvFaAgF6KuvVSQaMFqrZpusqjldL1sq_Qqdp3vvZtXzU9OKWl1a6Sa3KzcMunXucyu9kN2eM42oBxzoZrDYLXjdbFyherTzHnhL3Zp2Fn05vhYA4pmCUFU5ZrDikYXnquj_jZ7bD71_F37cUgFkMupbDFZF7inEIZ-T_Ud9R7lPw</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Kusat Ol, Kevser</creator><creator>Kanbak, Güngör</creator><creator>Oğlakcı Ilhan, Ayşegül</creator><creator>Burukoglu, Dilek</creator><creator>Yücel, Ferruh</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160301</creationdate><title>The investigation of the prenatal and postnatal alcohol exposure-induced neurodegeneration in rat brain: protection by betaine and/or omega-3</title><author>Kusat Ol, Kevser ; Kanbak, Güngör ; Oğlakcı Ilhan, Ayşegül ; Burukoglu, Dilek ; Yücel, Ferruh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-2b0b31640e0ec3269c340872e4988d5cea33bf3f5eb46cdc6c9f58f0b473a7b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Betaine - pharmacology</topic><topic>Brain - drug effects</topic><topic>Central Nervous System Depressants - toxicity</topic><topic>Disease Models, Animal</topic><topic>Ethanol - toxicity</topic><topic>Fatty Acids, Omega-3 - pharmacology</topic><topic>Female</topic><topic>Lipotropic Agents - pharmacology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nerve Degeneration - chemically induced</topic><topic>Nerve Degeneration - prevention & control</topic><topic>Neurosciences</topic><topic>Neurosurgery</topic><topic>Original Paper</topic><topic>Pregnancy</topic><topic>Prenatal Exposure Delayed Effects - chemically induced</topic><topic>Prenatal Exposure Delayed Effects - prevention & control</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kusat Ol, Kevser</creatorcontrib><creatorcontrib>Kanbak, Güngör</creatorcontrib><creatorcontrib>Oğlakcı Ilhan, Ayşegül</creatorcontrib><creatorcontrib>Burukoglu, Dilek</creatorcontrib><creatorcontrib>Yücel, Ferruh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Child's nervous system</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kusat Ol, Kevser</au><au>Kanbak, Güngör</au><au>Oğlakcı Ilhan, Ayşegül</au><au>Burukoglu, Dilek</au><au>Yücel, Ferruh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The investigation of the prenatal and postnatal alcohol exposure-induced neurodegeneration in rat brain: protection by betaine and/or omega-3</atitle><jtitle>Child's nervous system</jtitle><stitle>Childs Nerv Syst</stitle><addtitle>Childs Nerv Syst</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>32</volume><issue>3</issue><spage>467</spage><epage>474</epage><pages>467-474</pages><issn>0256-7040</issn><eissn>1433-0350</eissn><abstract>Purpose
We aim to study the effect of neurodegeneration on the brain of rat pups caused by prenatal and postnatal ethanol exposure with modified liquid diet to elucidate protective effects of betaine and omega-3 supplementation. When ethanol is consumed during prenatal and postnatal periods, it may result in fetal alcohol syndrome (FAS) in the offspring.
Methods
Rats were divided into control, ethanol, ethanol + betaine, ethanol + omega-3, ethanol + omega-3 + betaine groups. The effect of betaine and omega-3 in response to ethanol-induced changes on the brain, by biochemical analyses cytochrome c, caspase-3, calpain, cathepsin B and L, DNA fragmentation, histological and morfometric methods were evaluated.
Results
Caspase-3, calpain, cathepsin B, and cytochrome c levels in ethanol group were significantly higher than control. Caspase-3, calpain levels were decreased in ethanol + betaine, ethanol + omega-3, and ethanol + omega-3 + betaine groups compared to ethanol group. Cathepsin B in ethanol + omega-3 + betaine group was decreased compared to ethanol, ethanol + betaine groups. Cathepsin L and DNA fragmentation were found not statistically significant. We found similar results in histological and morfometric parameters.
Conclusion
We found that pre- and postnatal ethanol exposure is capable of triggering necrotic cell death in rat brains, omega-3, and betaine reduce neurodegeneration. Omega-3 and betaine may prove beneficial for neurodegeneration, particularly in preventing FAS.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26732065</pmid><doi>10.1007/s00381-015-2990-1</doi><tpages>8</tpages></addata></record> |
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| ispartof | Child's nervous system, 2016-03, Vol.32 (3), p.467-474 |
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| subjects | Animals Betaine - pharmacology Brain - drug effects Central Nervous System Depressants - toxicity Disease Models, Animal Ethanol - toxicity Fatty Acids, Omega-3 - pharmacology Female Lipotropic Agents - pharmacology Medicine Medicine & Public Health Nerve Degeneration - chemically induced Nerve Degeneration - prevention & control Neurosciences Neurosurgery Original Paper Pregnancy Prenatal Exposure Delayed Effects - chemically induced Prenatal Exposure Delayed Effects - prevention & control Rats Rats, Sprague-Dawley |
| title | The investigation of the prenatal and postnatal alcohol exposure-induced neurodegeneration in rat brain: protection by betaine and/or omega-3 |
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