Biophysical and pharmacological characterization of voltage-sensitive calcium currents in neonatal rat inferior colliculus neurons
Calcium conductances have been found in neonatal inferior colliculus neurons, however the biophysical and pharmacological profiles of the underlying calcium currents have not yet been characterized. In this study, we examined which types of voltage-activated calcium currents comprise the whole-cell...
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| description | Calcium conductances have been found in neonatal inferior colliculus neurons, however the biophysical and pharmacological profiles of the underlying calcium currents have not yet been characterized. In this study, we examined which types of voltage-activated calcium currents comprise the whole-cell inward current of neonatal inferior colliculus neurons (10–22
μm in diameter). On the basis of their voltage-dependence and pharmacological sensitivities, three major components of barium currents were identified. A low threshold voltage-activated current that activated around −70
mV, a mid threshold voltage-activated current that activated near −50
mV, and a high threshold voltage-activated current that activated around −40
mV. Low and mid threshold voltage-activated currents were present in 33% and 41% of the recordings, respectively, whereas high threshold voltage-activated currents were recorded in all inferior colliculus neurons tested. Nickel chloride (50
μM) and U-92032 (1
μM), which both block low threshold voltage-activated currents, reduced the amplitude of low threshold voltage-activated peak currents at a test potential of −60
mV by 72% and 10%, respectively. In addition, 50
μM nickel chloride and 1
μM U-92032 reduced the amplitude of mid threshold voltage-activated peak currents measured at −20
mV by 55% and 21%, respectively. Further pharmacological analysis indicated the presence of multiple types of high threshold voltage-activated currents in neonatal inferior colliculus neurons. The dihydropyridine nimodipine (1
μM), a selective L-type current antagonist, reduced the amplitude of high threshold voltage-activated peak currents by 25%. In addition, FPL 64176 (1
μM), a non-dihydropyridine L-type current agonist caused a dramatic 534% increase in the amplitude of the slow sustained component of the tail current measured at −40
mV. These data indicate that inferior colliculus neurons express L-type channels. ω-Conotoxin GVIA (1
μM), a selective blocker of N-type current, inhibited high threshold voltage-activated peak currents by 28% indicating the presence of N-type channels. ω-Agatoxin IVA (300
nM), a potent P/Q-type antagonist, reduced high threshold voltage-activated peak currents by 27%, suggesting that inferior colliculus neurons express P/Q-type channels. Concomitant application of nimodipine (1
μM), ω-conotoxin GVIA (1
μM) and ω-agatoxin IVA (300
nM) onto inferior colliculus neurons decreased the control high threshold voltage-activated peak |
| doi_str_mv | 10.1016/S0306-4522(00)00006-3 |
| format | Article |
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μm in diameter). On the basis of their voltage-dependence and pharmacological sensitivities, three major components of barium currents were identified. A low threshold voltage-activated current that activated around −70
mV, a mid threshold voltage-activated current that activated near −50
mV, and a high threshold voltage-activated current that activated around −40
mV. Low and mid threshold voltage-activated currents were present in 33% and 41% of the recordings, respectively, whereas high threshold voltage-activated currents were recorded in all inferior colliculus neurons tested. Nickel chloride (50
μM) and U-92032 (1
μM), which both block low threshold voltage-activated currents, reduced the amplitude of low threshold voltage-activated peak currents at a test potential of −60
mV by 72% and 10%, respectively. In addition, 50
μM nickel chloride and 1
μM U-92032 reduced the amplitude of mid threshold voltage-activated peak currents measured at −20
mV by 55% and 21%, respectively. Further pharmacological analysis indicated the presence of multiple types of high threshold voltage-activated currents in neonatal inferior colliculus neurons. The dihydropyridine nimodipine (1
μM), a selective L-type current antagonist, reduced the amplitude of high threshold voltage-activated peak currents by 25%. In addition, FPL 64176 (1
μM), a non-dihydropyridine L-type current agonist caused a dramatic 534% increase in the amplitude of the slow sustained component of the tail current measured at −40
mV. These data indicate that inferior colliculus neurons express L-type channels. ω-Conotoxin GVIA (1
μM), a selective blocker of N-type current, inhibited high threshold voltage-activated peak currents by 28% indicating the presence of N-type channels. ω-Agatoxin IVA (300
nM), a potent P/Q-type antagonist, reduced high threshold voltage-activated peak currents by 27%, suggesting that inferior colliculus neurons express P/Q-type channels. Concomitant application of nimodipine (1
μM), ω-conotoxin GVIA (1
μM) and ω-agatoxin IVA (300
nM) onto inferior colliculus neurons decreased the control high threshold voltage-activated peak currents only by 62%.
Thus, inferior colliculus neurons may express at least one more type of calcium current in addition to low and mid threshold voltage-activated currents and L-type, N-type and P/Q-type high threshold currents.</description><identifier>ISSN: 0306-4522</identifier><identifier>EISSN: 1873-7544</identifier><identifier>DOI: 10.1016/S0306-4522(00)00006-3</identifier><identifier>PMID: 10727793</identifier><identifier>CODEN: NRSCDN</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Animals, Newborn ; Barium - metabolism ; Barium - pharmacology ; Biological and medical sciences ; calcium channel ; Calcium Channel Blockers - pharmacology ; Calcium Channels - drug effects ; Calcium Channels - physiology ; dihydropyridine ; Drug Combinations ; Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation ; Female ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Inferior Colliculi - cytology ; Inferior Colliculi - drug effects ; Inferior Colliculi - metabolism ; Male ; Neurons - cytology ; Neurons - drug effects ; Neurons - metabolism ; nickel ; Nickel - pharmacology ; Nimodipine - pharmacology ; omega-Agatoxin IVA - pharmacology ; omega-Conotoxin GVIA - pharmacology ; Patch-Clamp Techniques ; Piperazines - pharmacology ; Rats ; Rats, Sprague-Dawley ; Tropolone - analogs & derivatives ; Tropolone - pharmacology ; U-92032 ; Vertebrates: nervous system and sense organs ; ω-agatoxin ; ω-conotoxin GVIA</subject><ispartof>Neuroscience, 2000-01, Vol.96 (4), p.753-765</ispartof><rights>2000 IBRO</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-95e5ca6c437d46818253592d89802662fbcae479db6580de3b559fb748fa44ac3</citedby><cites>FETCH-LOGICAL-c473t-95e5ca6c437d46818253592d89802662fbcae479db6580de3b559fb748fa44ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0306-4522(00)00006-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1317466$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10727793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>N'Gouemo, P.</creatorcontrib><creatorcontrib>Rittenhouse, A.R.</creatorcontrib><title>Biophysical and pharmacological characterization of voltage-sensitive calcium currents in neonatal rat inferior colliculus neurons</title><title>Neuroscience</title><addtitle>Neuroscience</addtitle><description>Calcium conductances have been found in neonatal inferior colliculus neurons, however the biophysical and pharmacological profiles of the underlying calcium currents have not yet been characterized. In this study, we examined which types of voltage-activated calcium currents comprise the whole-cell inward current of neonatal inferior colliculus neurons (10–22
μm in diameter). On the basis of their voltage-dependence and pharmacological sensitivities, three major components of barium currents were identified. A low threshold voltage-activated current that activated around −70
mV, a mid threshold voltage-activated current that activated near −50
mV, and a high threshold voltage-activated current that activated around −40
mV. Low and mid threshold voltage-activated currents were present in 33% and 41% of the recordings, respectively, whereas high threshold voltage-activated currents were recorded in all inferior colliculus neurons tested. Nickel chloride (50
μM) and U-92032 (1
μM), which both block low threshold voltage-activated currents, reduced the amplitude of low threshold voltage-activated peak currents at a test potential of −60
mV by 72% and 10%, respectively. In addition, 50
μM nickel chloride and 1
μM U-92032 reduced the amplitude of mid threshold voltage-activated peak currents measured at −20
mV by 55% and 21%, respectively. Further pharmacological analysis indicated the presence of multiple types of high threshold voltage-activated currents in neonatal inferior colliculus neurons. The dihydropyridine nimodipine (1
μM), a selective L-type current antagonist, reduced the amplitude of high threshold voltage-activated peak currents by 25%. In addition, FPL 64176 (1
μM), a non-dihydropyridine L-type current agonist caused a dramatic 534% increase in the amplitude of the slow sustained component of the tail current measured at −40
mV. These data indicate that inferior colliculus neurons express L-type channels. ω-Conotoxin GVIA (1
μM), a selective blocker of N-type current, inhibited high threshold voltage-activated peak currents by 28% indicating the presence of N-type channels. ω-Agatoxin IVA (300
nM), a potent P/Q-type antagonist, reduced high threshold voltage-activated peak currents by 27%, suggesting that inferior colliculus neurons express P/Q-type channels. Concomitant application of nimodipine (1
μM), ω-conotoxin GVIA (1
μM) and ω-agatoxin IVA (300
nM) onto inferior colliculus neurons decreased the control high threshold voltage-activated peak currents only by 62%.
Thus, inferior colliculus neurons may express at least one more type of calcium current in addition to low and mid threshold voltage-activated currents and L-type, N-type and P/Q-type high threshold currents.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Barium - metabolism</subject><subject>Barium - pharmacology</subject><subject>Biological and medical sciences</subject><subject>calcium channel</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium Channels - drug effects</subject><subject>Calcium Channels - physiology</subject><subject>dihydropyridine</subject><subject>Drug Combinations</subject><subject>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Inferior Colliculi - cytology</subject><subject>Inferior Colliculi - drug effects</subject><subject>Inferior Colliculi - metabolism</subject><subject>Male</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>nickel</subject><subject>Nickel - pharmacology</subject><subject>Nimodipine - pharmacology</subject><subject>omega-Agatoxin IVA - pharmacology</subject><subject>omega-Conotoxin GVIA - pharmacology</subject><subject>Patch-Clamp Techniques</subject><subject>Piperazines - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Tropolone - analogs & derivatives</subject><subject>Tropolone - pharmacology</subject><subject>U-92032</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>ω-agatoxin</subject><subject>ω-conotoxin GVIA</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEuLFTEQRoMozp3Rn6BkIaKL1qTz6l6JDuMDBlyo61Cdrp6JdCfXJH1hXPrLzX2g7qxNUeHUl-IQ8oSzV5xx_foLE0w3UrXtC8Zeslq6EffIhndGNEZJeZ9s_iBn5Dzn73tISfGQnHFmWmN6sSG_3vm4vb3L3sFMIYx0ewtpARfneHN4c3UGVzD5n1B8DDROdBfnAjfYZAzZF79DWknn14W6NSUMJVMfaMAYoNSIBKXOU42Iidbk2bt1XnMF1hRDfkQeTDBnfHzqF-Tb-6uvlx-b688fPl2-vW6cNKI0vULlQDspzCh1x7tWCdW3Y9d3rNW6nQYHKE0_Dlp1bEQxKNVPg5HdBFKCExfk-TF3m-KPFXOxi88O5xnqpWu23Oi-511fQXUEXYo5J5zsNvkF0p3lzO7l24N8uzdrGbMH-VbUvaenD9ZhwfGfraPtCjw7AZCrsSlBcD7_5QQ3UuuKvTliWG3sPCabncfgcPQJXbFj9P-55DdTR6Pq</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>N'Gouemo, P.</creator><creator>Rittenhouse, A.R.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope></search><sort><creationdate>20000101</creationdate><title>Biophysical and pharmacological characterization of voltage-sensitive calcium currents in neonatal rat inferior colliculus neurons</title><author>N'Gouemo, P. ; Rittenhouse, A.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-95e5ca6c437d46818253592d89802662fbcae479db6580de3b559fb748fa44ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Barium - metabolism</topic><topic>Barium - pharmacology</topic><topic>Biological and medical sciences</topic><topic>calcium channel</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium Channels - drug effects</topic><topic>Calcium Channels - physiology</topic><topic>dihydropyridine</topic><topic>Drug Combinations</topic><topic>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Inferior Colliculi - cytology</topic><topic>Inferior Colliculi - drug effects</topic><topic>Inferior Colliculi - metabolism</topic><topic>Male</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>nickel</topic><topic>Nickel - pharmacology</topic><topic>Nimodipine - pharmacology</topic><topic>omega-Agatoxin IVA - pharmacology</topic><topic>omega-Conotoxin GVIA - pharmacology</topic><topic>Patch-Clamp Techniques</topic><topic>Piperazines - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Tropolone - analogs & derivatives</topic><topic>Tropolone - pharmacology</topic><topic>U-92032</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>ω-agatoxin</topic><topic>ω-conotoxin GVIA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>N'Gouemo, P.</creatorcontrib><creatorcontrib>Rittenhouse, A.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>N'Gouemo, P.</au><au>Rittenhouse, A.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biophysical and pharmacological characterization of voltage-sensitive calcium currents in neonatal rat inferior colliculus neurons</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>96</volume><issue>4</issue><spage>753</spage><epage>765</epage><pages>753-765</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><coden>NRSCDN</coden><abstract>Calcium conductances have been found in neonatal inferior colliculus neurons, however the biophysical and pharmacological profiles of the underlying calcium currents have not yet been characterized. In this study, we examined which types of voltage-activated calcium currents comprise the whole-cell inward current of neonatal inferior colliculus neurons (10–22
μm in diameter). On the basis of their voltage-dependence and pharmacological sensitivities, three major components of barium currents were identified. A low threshold voltage-activated current that activated around −70
mV, a mid threshold voltage-activated current that activated near −50
mV, and a high threshold voltage-activated current that activated around −40
mV. Low and mid threshold voltage-activated currents were present in 33% and 41% of the recordings, respectively, whereas high threshold voltage-activated currents were recorded in all inferior colliculus neurons tested. Nickel chloride (50
μM) and U-92032 (1
μM), which both block low threshold voltage-activated currents, reduced the amplitude of low threshold voltage-activated peak currents at a test potential of −60
mV by 72% and 10%, respectively. In addition, 50
μM nickel chloride and 1
μM U-92032 reduced the amplitude of mid threshold voltage-activated peak currents measured at −20
mV by 55% and 21%, respectively. Further pharmacological analysis indicated the presence of multiple types of high threshold voltage-activated currents in neonatal inferior colliculus neurons. The dihydropyridine nimodipine (1
μM), a selective L-type current antagonist, reduced the amplitude of high threshold voltage-activated peak currents by 25%. In addition, FPL 64176 (1
μM), a non-dihydropyridine L-type current agonist caused a dramatic 534% increase in the amplitude of the slow sustained component of the tail current measured at −40
mV. These data indicate that inferior colliculus neurons express L-type channels. ω-Conotoxin GVIA (1
μM), a selective blocker of N-type current, inhibited high threshold voltage-activated peak currents by 28% indicating the presence of N-type channels. ω-Agatoxin IVA (300
nM), a potent P/Q-type antagonist, reduced high threshold voltage-activated peak currents by 27%, suggesting that inferior colliculus neurons express P/Q-type channels. Concomitant application of nimodipine (1
μM), ω-conotoxin GVIA (1
μM) and ω-agatoxin IVA (300
nM) onto inferior colliculus neurons decreased the control high threshold voltage-activated peak currents only by 62%.
Thus, inferior colliculus neurons may express at least one more type of calcium current in addition to low and mid threshold voltage-activated currents and L-type, N-type and P/Q-type high threshold currents.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10727793</pmid><doi>10.1016/S0306-4522(00)00006-3</doi><tpages>13</tpages></addata></record> |
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| ispartof | Neuroscience, 2000-01, Vol.96 (4), p.753-765 |
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| subjects | Animals Animals, Newborn Barium - metabolism Barium - pharmacology Biological and medical sciences calcium channel Calcium Channel Blockers - pharmacology Calcium Channels - drug effects Calcium Channels - physiology dihydropyridine Drug Combinations Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation Female Fundamental and applied biological sciences. Psychology In Vitro Techniques Inferior Colliculi - cytology Inferior Colliculi - drug effects Inferior Colliculi - metabolism Male Neurons - cytology Neurons - drug effects Neurons - metabolism nickel Nickel - pharmacology Nimodipine - pharmacology omega-Agatoxin IVA - pharmacology omega-Conotoxin GVIA - pharmacology Patch-Clamp Techniques Piperazines - pharmacology Rats Rats, Sprague-Dawley Tropolone - analogs & derivatives Tropolone - pharmacology U-92032 Vertebrates: nervous system and sense organs ω-agatoxin ω-conotoxin GVIA |
| title | Biophysical and pharmacological characterization of voltage-sensitive calcium currents in neonatal rat inferior colliculus neurons |
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