Individual Nucleotide Bases, Not Base Pairs, Are Critical for Triggering Site-specific DNA Cleavage by Vaccinia Topoisomerase

Vaccinia DNA topoisomerase forms a covalent DNA-(3′-phosphotyrosyl)-enzyme intermediate at a specific target site 5′-C+5C+4C+3T+2T+1p↓N-1 in duplex DNA. Here we study the effects of abasic lesions at individual positions of the scissile and nonscissile strands on the rate of single-turnover DNA tran...

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Veröffentlicht in:	The Journal of biological chemistry 2004-09, Vol.279 (38), p.39718-39726
Hauptverfasser:	Tian, Ligeng, Sayer, Jane M., Jerina, Donald M., Shuman, Stewart
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Binding Sites - genetics DNA - chemistry DNA - metabolism DNA Topoisomerases, Type I - genetics DNA Topoisomerases, Type I - metabolism Molecular Sequence Data Nucleotides - chemistry Nucleotides - metabolism Substrate Specificity Vaccinia - enzymology Vaccinia - genetics Viral Proteins - genetics Viral Proteins - metabolism
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