Ability of Polycyclic Aromatic Hydrocarbons to Induce 7-Ethoxyresorufin-o-deethylase Activity in a Trout Liver Cell Line
Along with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 24 unsubstituted polycyclic aromatic hydrocarbons (PAHs) were evaluated for their ability to induce 7-ethoxyresorufin-o-deethylase (EROD) activity in the rainbow trout liver cell line RTL-W1. When the duration and cell density of exposure were i...
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| Veröffentlicht in: | Ecotoxicology and environmental safety 1999-09, Vol.44 (1), p.118-128 |
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| creator | Bols, N.C. Schirmer, K. Joyce, E.M. Dixon, D.G. Greenberg, B.M. Whyte, J.J. |
| description | Along with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 24 unsubstituted polycyclic aromatic hydrocarbons (PAHs) were evaluated for their ability to induce 7-ethoxyresorufin-o-deethylase (EROD) activity in the rainbow trout liver cell line RTL-W1. When the duration and cell density of exposure were increased, the EC50 for EROD induction was relatively constant for TCDD, but increased for PAHs. Regardless of exposure conditions, EROD activity was not induced by 9 PAHs: naphthalene, phenanthrene, anthracene, pyrene, perylene, acenaphthylene, acenaphthene, fluorene, and fluoranthene. Two PAHs, benzo[g,h,i]perylene and coronene, induced EROD activity inconsistently. The remaining 13 PAHs consistently induced EROD activity. The EC50s for induction exhibited approximately a 110-fold range. The order of potency, from most to least potent, was benzo[k]fluoranthene, dibenzo[a,i]pyrene, dibenzo [a,h]anthracene, benzo[a]pyrene, indeno[1,2,3-cd]pyrene, benzo [b]fluoranthene, pentacene, benzo[b]anthracene, benzo[b] fluorene, chrysene, benzo[a]anthracene, benzo[e]pyrene, and triphenylene. When the induction potency was expressed relative to TCDD, the toxic equivalency factors (TEFs) ranged from 0.001 to 0.000 01. When expressed relative to benzo[a]pyrene, the TEFs ranged from 3.44 to 0.03. |
| doi_str_mv | 10.1006/eesa.1999.1808 |
| format | Article |
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When the duration and cell density of exposure were increased, the EC50 for EROD induction was relatively constant for TCDD, but increased for PAHs. Regardless of exposure conditions, EROD activity was not induced by 9 PAHs: naphthalene, phenanthrene, anthracene, pyrene, perylene, acenaphthylene, acenaphthene, fluorene, and fluoranthene. Two PAHs, benzo[g,h,i]perylene and coronene, induced EROD activity inconsistently. The remaining 13 PAHs consistently induced EROD activity. The EC50s for induction exhibited approximately a 110-fold range. The order of potency, from most to least potent, was benzo[k]fluoranthene, dibenzo[a,i]pyrene, dibenzo [a,h]anthracene, benzo[a]pyrene, indeno[1,2,3-cd]pyrene, benzo [b]fluoranthene, pentacene, benzo[b]anthracene, benzo[b] fluorene, chrysene, benzo[a]anthracene, benzo[e]pyrene, and triphenylene. When the induction potency was expressed relative to TCDD, the toxic equivalency factors (TEFs) ranged from 0.001 to 0.000 01. When expressed relative to benzo[a]pyrene, the TEFs ranged from 3.44 to 0.03.</description><identifier>ISSN: 0147-6513</identifier><identifier>EISSN: 1090-2414</identifier><identifier>DOI: 10.1006/eesa.1999.1808</identifier><identifier>PMID: 10499998</identifier><identifier>CODEN: EESADV</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>7-ethoxyresorufin O-deethylase ; Agnatha. Pisces ; Animal, plant and microbial ecology ; Animals ; Applied ecology ; benzo(k)fluoranthene ; Biological and medical sciences ; Cell Line ; Cytochrome P-450 CYP1A1 - drug effects ; Cytochrome P-450 CYP1A1 - metabolism ; Dibenzo(a,l)pyrene ; Ecotoxicology, biological effects of pollution ; Effects of pollution and side effects of pesticides on vertebrates ; Enzyme Induction ; EROD ; Fundamental and applied biological sciences. Psychology ; Hydrocarbons, Aromatic - pharmacology ; Hydrocarbons, Aromatic - toxicity ; Liver - enzymology ; Oncorhynchus mykiss ; Oncorhynchus mykiss - physiology ; polycyclic aromatic hydrocarbons ; Polycyclic Compounds - pharmacology ; Polycyclic Compounds - toxicity ; rainbow trout ; Reference Values ; RTL-W1 ; toxic equivalency factors ; Water Pollutants, Chemical - pharmacology ; Water Pollutants, Chemical - toxicity</subject><ispartof>Ecotoxicology and environmental safety, 1999-09, Vol.44 (1), p.118-128</ispartof><rights>1999 Academic Press</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1999 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-be8f528ea581c28ef0d616d8c9f13c15320cf72e6877c8f38273276c2595fb043</citedby><cites>FETCH-LOGICAL-c528t-be8f528ea581c28ef0d616d8c9f13c15320cf72e6877c8f38273276c2595fb043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0147651399918080$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1957589$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10499998$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bols, N.C.</creatorcontrib><creatorcontrib>Schirmer, K.</creatorcontrib><creatorcontrib>Joyce, E.M.</creatorcontrib><creatorcontrib>Dixon, D.G.</creatorcontrib><creatorcontrib>Greenberg, B.M.</creatorcontrib><creatorcontrib>Whyte, J.J.</creatorcontrib><title>Ability of Polycyclic Aromatic Hydrocarbons to Induce 7-Ethoxyresorufin-o-deethylase Activity in a Trout Liver Cell Line</title><title>Ecotoxicology and environmental safety</title><addtitle>Ecotoxicol Environ Saf</addtitle><description>Along with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 24 unsubstituted polycyclic aromatic hydrocarbons (PAHs) were evaluated for their ability to induce 7-ethoxyresorufin-o-deethylase (EROD) activity in the rainbow trout liver cell line RTL-W1. When the duration and cell density of exposure were increased, the EC50 for EROD induction was relatively constant for TCDD, but increased for PAHs. Regardless of exposure conditions, EROD activity was not induced by 9 PAHs: naphthalene, phenanthrene, anthracene, pyrene, perylene, acenaphthylene, acenaphthene, fluorene, and fluoranthene. Two PAHs, benzo[g,h,i]perylene and coronene, induced EROD activity inconsistently. The remaining 13 PAHs consistently induced EROD activity. The EC50s for induction exhibited approximately a 110-fold range. The order of potency, from most to least potent, was benzo[k]fluoranthene, dibenzo[a,i]pyrene, dibenzo [a,h]anthracene, benzo[a]pyrene, indeno[1,2,3-cd]pyrene, benzo [b]fluoranthene, pentacene, benzo[b]anthracene, benzo[b] fluorene, chrysene, benzo[a]anthracene, benzo[e]pyrene, and triphenylene. When the induction potency was expressed relative to TCDD, the toxic equivalency factors (TEFs) ranged from 0.001 to 0.000 01. When expressed relative to benzo[a]pyrene, the TEFs ranged from 3.44 to 0.03.</description><subject>7-ethoxyresorufin O-deethylase</subject><subject>Agnatha. Pisces</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>benzo(k)fluoranthene</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cytochrome P-450 CYP1A1 - drug effects</subject><subject>Cytochrome P-450 CYP1A1 - metabolism</subject><subject>Dibenzo(a,l)pyrene</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effects of pollution and side effects of pesticides on vertebrates</subject><subject>Enzyme Induction</subject><subject>EROD</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrocarbons, Aromatic - pharmacology</subject><subject>Hydrocarbons, Aromatic - toxicity</subject><subject>Liver - enzymology</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - physiology</subject><subject>polycyclic aromatic hydrocarbons</subject><subject>Polycyclic Compounds - pharmacology</subject><subject>Polycyclic Compounds - toxicity</subject><subject>rainbow trout</subject><subject>Reference Values</subject><subject>RTL-W1</subject><subject>toxic equivalency factors</subject><subject>Water Pollutants, Chemical - pharmacology</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0147-6513</issn><issn>1090-2414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhiMEokvhyhH5gLhlsZP467haFVppJTiUs-VMxqpRNi62s2r-fR3tSnBB6mnewzOvxn6q6iOjW0ap-IqY7JZprbdMUfWq2jCqad10rHtdbSjrZC04a6-qdyn9ppS2lPO31RWjXVnRalM97Xo_-ryQ4MjPMC6wwOiB7GI42lzC7TLEADb2YUokB3I3DTMgkfVNfghPS8QU4uz8VId6QMwPy2gTkh1kf1pb_UQsuY9hzuTgTxjJHsexxAnfV2-cHRN-uMzr6te3m_v9bX348f1uvzvUwBuV6x6VKwEtVwzKdHQQTAwKtGMtMN42FJxsUCgpQblWNbJtpICGa-562rXX1Zdz72MMf2ZM2Rx9gnKFnTDMyTApNBVavwBspRLqBY0dZ0wJWcDtGYQYUorozGP0RxsXw6hZ7ZnVnlntmdVeWfh0aZ77Iw7_4GddBfh8AWwCO7poJ_DpL6e55Gp9ijpjWD725DGaBB4nwMFHhGyG4P93wjMS0LX9</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>Bols, N.C.</creator><creator>Schirmer, K.</creator><creator>Joyce, E.M.</creator><creator>Dixon, D.G.</creator><creator>Greenberg, B.M.</creator><creator>Whyte, J.J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>7QH</scope></search><sort><creationdate>19990901</creationdate><title>Ability of Polycyclic Aromatic Hydrocarbons to Induce 7-Ethoxyresorufin-o-deethylase Activity in a Trout Liver Cell Line</title><author>Bols, N.C. ; Schirmer, K. ; Joyce, E.M. ; Dixon, D.G. ; Greenberg, B.M. ; Whyte, J.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-be8f528ea581c28ef0d616d8c9f13c15320cf72e6877c8f38273276c2595fb043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>7-ethoxyresorufin O-deethylase</topic><topic>Agnatha. Pisces</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>benzo(k)fluoranthene</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cytochrome P-450 CYP1A1 - drug effects</topic><topic>Cytochrome P-450 CYP1A1 - metabolism</topic><topic>Dibenzo(a,l)pyrene</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Effects of pollution and side effects of pesticides on vertebrates</topic><topic>Enzyme Induction</topic><topic>EROD</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrocarbons, Aromatic - pharmacology</topic><topic>Hydrocarbons, Aromatic - toxicity</topic><topic>Liver - enzymology</topic><topic>Oncorhynchus mykiss</topic><topic>Oncorhynchus mykiss - physiology</topic><topic>polycyclic aromatic hydrocarbons</topic><topic>Polycyclic Compounds - pharmacology</topic><topic>Polycyclic Compounds - toxicity</topic><topic>rainbow trout</topic><topic>Reference Values</topic><topic>RTL-W1</topic><topic>toxic equivalency factors</topic><topic>Water Pollutants, Chemical - pharmacology</topic><topic>Water Pollutants, Chemical - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bols, N.C.</creatorcontrib><creatorcontrib>Schirmer, K.</creatorcontrib><creatorcontrib>Joyce, E.M.</creatorcontrib><creatorcontrib>Dixon, D.G.</creatorcontrib><creatorcontrib>Greenberg, B.M.</creatorcontrib><creatorcontrib>Whyte, J.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Aqualine</collection><jtitle>Ecotoxicology and environmental safety</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bols, N.C.</au><au>Schirmer, K.</au><au>Joyce, E.M.</au><au>Dixon, D.G.</au><au>Greenberg, B.M.</au><au>Whyte, J.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ability of Polycyclic Aromatic Hydrocarbons to Induce 7-Ethoxyresorufin-o-deethylase Activity in a Trout Liver Cell Line</atitle><jtitle>Ecotoxicology and environmental safety</jtitle><addtitle>Ecotoxicol Environ Saf</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>44</volume><issue>1</issue><spage>118</spage><epage>128</epage><pages>118-128</pages><issn>0147-6513</issn><eissn>1090-2414</eissn><coden>EESADV</coden><abstract>Along with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 24 unsubstituted polycyclic aromatic hydrocarbons (PAHs) were evaluated for their ability to induce 7-ethoxyresorufin-o-deethylase (EROD) activity in the rainbow trout liver cell line RTL-W1. When the duration and cell density of exposure were increased, the EC50 for EROD induction was relatively constant for TCDD, but increased for PAHs. Regardless of exposure conditions, EROD activity was not induced by 9 PAHs: naphthalene, phenanthrene, anthracene, pyrene, perylene, acenaphthylene, acenaphthene, fluorene, and fluoranthene. Two PAHs, benzo[g,h,i]perylene and coronene, induced EROD activity inconsistently. The remaining 13 PAHs consistently induced EROD activity. The EC50s for induction exhibited approximately a 110-fold range. The order of potency, from most to least potent, was benzo[k]fluoranthene, dibenzo[a,i]pyrene, dibenzo [a,h]anthracene, benzo[a]pyrene, indeno[1,2,3-cd]pyrene, benzo [b]fluoranthene, pentacene, benzo[b]anthracene, benzo[b] fluorene, chrysene, benzo[a]anthracene, benzo[e]pyrene, and triphenylene. When the induction potency was expressed relative to TCDD, the toxic equivalency factors (TEFs) ranged from 0.001 to 0.000 01. When expressed relative to benzo[a]pyrene, the TEFs ranged from 3.44 to 0.03.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>10499998</pmid><doi>10.1006/eesa.1999.1808</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Ecotoxicology and environmental safety, 1999-09, Vol.44 (1), p.118-128 |
| issn | 0147-6513 1090-2414 |
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| subjects | 7-ethoxyresorufin O-deethylase Agnatha. Pisces Animal, plant and microbial ecology Animals Applied ecology benzo(k)fluoranthene Biological and medical sciences Cell Line Cytochrome P-450 CYP1A1 - drug effects Cytochrome P-450 CYP1A1 - metabolism Dibenzo(a,l)pyrene Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on vertebrates Enzyme Induction EROD Fundamental and applied biological sciences. Psychology Hydrocarbons, Aromatic - pharmacology Hydrocarbons, Aromatic - toxicity Liver - enzymology Oncorhynchus mykiss Oncorhynchus mykiss - physiology polycyclic aromatic hydrocarbons Polycyclic Compounds - pharmacology Polycyclic Compounds - toxicity rainbow trout Reference Values RTL-W1 toxic equivalency factors Water Pollutants, Chemical - pharmacology Water Pollutants, Chemical - toxicity |
| title | Ability of Polycyclic Aromatic Hydrocarbons to Induce 7-Ethoxyresorufin-o-deethylase Activity in a Trout Liver Cell Line |
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