Structural and Environmental Features of Novel MdfA Variant and MdfA Genes in Recombinant Regions of Escherichia coli
Novel mdfA gene variants were identified simultaneously from 3 of 13 positive isolates of PCR amplification in Escherichia coli from patients. These 13 positive isolates showed resistance to chloramphenicol, tetracycline, and erythromycin. The 3 mdfA gene variants were of the same genotype and all t...
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| Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2014-10, Vol.20 (5), p.392-398 |
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| creator | Wang, Dongguo Liang, Haiyan Chen, Jiayu Mou, Yonghua Qi, Yongxiao |
| description | Novel
mdfA
gene variants were identified simultaneously from 3 of 13 positive isolates of PCR amplification in
Escherichia coli
from patients. These 13 positive isolates showed resistance to chloramphenicol, tetracycline, and erythromycin. The 3
mdfA
gene variants were of the same genotype and all the 13 positive isolates were investigated by conjugation experiment,
Eco
RI restriction, and gene mapping. Conjugation experiments demonstrated that the novel
mdfA
variant and
mdfA
genes were located on plasmids that were restricted by
Eco
RI for ∼8.2 kb-length, which was also validated by gene mapping. Further study indicated three types of genetic structures (A, B, and C) in the recombinant plasmids harboring
mdfA
and surrounding genes, and structure B was first reported in the article. Structure A comprises two partial-length and six full-length genes, including the
mdfA
gene variant in the recombinant plasmid; structure B comprises four full-length genes, the
mdfA
,
ybjG
,
dacC
, and
ybjI
; structure C comprises two full-length genes, the
mdfA
and
dacC
. These results suggested that the
mdfA
gene can function as transporter responsible for multidrug resistance and also mediated the synergistic function with its surrounding genes in conjugative plasmids. |
| doi_str_mv | 10.1089/mdr.2013.0201 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1768572740</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1768572740</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-8f9493a0ac56946fff751866f546f9fa8fb18a9768077f928b782e62b7a2c67c3</originalsourceid><addsrcrecordid>eNqFkUtP3DAUha2KqsCUJVsUiU03mfoVP5YIDQMSbSWgbCPHcw1GiU3tBKn_vs4MsOgGL_w49ztHsg5CxwQvCVb6-7BJS4oJW-Kyf0IHRDNSK87VXrljKWpBNd9Hhzk_YYwbItgXtE-5UJwqcYCm2zFNdpyS6SsTNtUqvPgUwwBhLMoFmDKCXEVX_Ywv0Fc_Nu6sujfJmzBuDVthDaFAPlQ3YOPQ-TBPb-DBx7D1rrJ9hOTtozeVjb3_ij4702c4ej0X6PfF6u78sr7-tb46P7uuLdNqrJXTXDODjW2E5sI5JxuihHBNeWhnlOuIMloKhaV0mqpOKgqCdtJQK6RlC_Rtl_uc4p8J8tgOPlvoexMgTrklxdpIKjn-GG2EIIRxRgp6-h_6FKcUykdmqilLY1WoekfZFHNO4Nrn5AeT_rYEt3N1bamunatr5-oKf_KaOnUDbN7pt64KwHbALJsQeg8dpPGD2H8k16Q4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1565555908</pqid></control><display><type>article</type><title>Structural and Environmental Features of Novel MdfA Variant and MdfA Genes in Recombinant Regions of Escherichia coli</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Wang, Dongguo ; Liang, Haiyan ; Chen, Jiayu ; Mou, Yonghua ; Qi, Yongxiao</creator><creatorcontrib>Wang, Dongguo ; Liang, Haiyan ; Chen, Jiayu ; Mou, Yonghua ; Qi, Yongxiao</creatorcontrib><description>Novel
mdfA
gene variants were identified simultaneously from 3 of 13 positive isolates of PCR amplification in
Escherichia coli
from patients. These 13 positive isolates showed resistance to chloramphenicol, tetracycline, and erythromycin. The 3
mdfA
gene variants were of the same genotype and all the 13 positive isolates were investigated by conjugation experiment,
Eco
RI restriction, and gene mapping. Conjugation experiments demonstrated that the novel
mdfA
variant and
mdfA
genes were located on plasmids that were restricted by
Eco
RI for ∼8.2 kb-length, which was also validated by gene mapping. Further study indicated three types of genetic structures (A, B, and C) in the recombinant plasmids harboring
mdfA
and surrounding genes, and structure B was first reported in the article. Structure A comprises two partial-length and six full-length genes, including the
mdfA
gene variant in the recombinant plasmid; structure B comprises four full-length genes, the
mdfA
,
ybjG
,
dacC
, and
ybjI
; structure C comprises two full-length genes, the
mdfA
and
dacC
. These results suggested that the
mdfA
gene can function as transporter responsible for multidrug resistance and also mediated the synergistic function with its surrounding genes in conjugative plasmids.</description><identifier>ISSN: 1076-6294</identifier><identifier>EISSN: 1931-8448</identifier><identifier>DOI: 10.1089/mdr.2013.0201</identifier><identifier>PMID: 24684286</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibiotics ; Biological Transport ; Chloramphenicol - pharmacology ; Conjugation, Genetic ; Deoxyribonuclease EcoRI - genetics ; Deoxyribonuclease EcoRI - metabolism ; Drug resistance ; Drug Resistance, Multiple, Bacterial - drug effects ; Drug Resistance, Multiple, Bacterial - genetics ; E coli ; Erythromycin - pharmacology ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Escherichia coli - metabolism ; Escherichia coli Infections - drug therapy ; Escherichia coli Infections - microbiology ; Escherichia coli Proteins - genetics ; Escherichia coli Proteins - metabolism ; Gene Expression Regulation, Bacterial ; Gene mapping ; Genetic recombination ; Genotype ; Humans ; Mechanisms ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Plasmids ; Plasmids - chemistry ; Plasmids - metabolism ; Serine-Type D-Ala-D-Ala Carboxypeptidase - genetics ; Serine-Type D-Ala-D-Ala Carboxypeptidase - metabolism ; Tetracycline - pharmacology</subject><ispartof>Microbial drug resistance (Larchmont, N.Y.), 2014-10, Vol.20 (5), p.392-398</ispartof><rights>2014, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2014, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-8f9493a0ac56946fff751866f546f9fa8fb18a9768077f928b782e62b7a2c67c3</citedby><cites>FETCH-LOGICAL-c398t-8f9493a0ac56946fff751866f546f9fa8fb18a9768077f928b782e62b7a2c67c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24684286$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Dongguo</creatorcontrib><creatorcontrib>Liang, Haiyan</creatorcontrib><creatorcontrib>Chen, Jiayu</creatorcontrib><creatorcontrib>Mou, Yonghua</creatorcontrib><creatorcontrib>Qi, Yongxiao</creatorcontrib><title>Structural and Environmental Features of Novel MdfA Variant and MdfA Genes in Recombinant Regions of Escherichia coli</title><title>Microbial drug resistance (Larchmont, N.Y.)</title><addtitle>Microb Drug Resist</addtitle><description>Novel
mdfA
gene variants were identified simultaneously from 3 of 13 positive isolates of PCR amplification in
Escherichia coli
from patients. These 13 positive isolates showed resistance to chloramphenicol, tetracycline, and erythromycin. The 3
mdfA
gene variants were of the same genotype and all the 13 positive isolates were investigated by conjugation experiment,
Eco
RI restriction, and gene mapping. Conjugation experiments demonstrated that the novel
mdfA
variant and
mdfA
genes were located on plasmids that were restricted by
Eco
RI for ∼8.2 kb-length, which was also validated by gene mapping. Further study indicated three types of genetic structures (A, B, and C) in the recombinant plasmids harboring
mdfA
and surrounding genes, and structure B was first reported in the article. Structure A comprises two partial-length and six full-length genes, including the
mdfA
gene variant in the recombinant plasmid; structure B comprises four full-length genes, the
mdfA
,
ybjG
,
dacC
, and
ybjI
; structure C comprises two full-length genes, the
mdfA
and
dacC
. These results suggested that the
mdfA
gene can function as transporter responsible for multidrug resistance and also mediated the synergistic function with its surrounding genes in conjugative plasmids.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Biological Transport</subject><subject>Chloramphenicol - pharmacology</subject><subject>Conjugation, Genetic</subject><subject>Deoxyribonuclease EcoRI - genetics</subject><subject>Deoxyribonuclease EcoRI - metabolism</subject><subject>Drug resistance</subject><subject>Drug Resistance, Multiple, Bacterial - drug effects</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>E coli</subject><subject>Erythromycin - pharmacology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli Infections - drug therapy</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene mapping</subject><subject>Genetic recombination</subject><subject>Genotype</subject><subject>Humans</subject><subject>Mechanisms</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Plasmids</subject><subject>Plasmids - chemistry</subject><subject>Plasmids - metabolism</subject><subject>Serine-Type D-Ala-D-Ala Carboxypeptidase - genetics</subject><subject>Serine-Type D-Ala-D-Ala Carboxypeptidase - metabolism</subject><subject>Tetracycline - pharmacology</subject><issn>1076-6294</issn><issn>1931-8448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUtP3DAUha2KqsCUJVsUiU03mfoVP5YIDQMSbSWgbCPHcw1GiU3tBKn_vs4MsOgGL_w49ztHsg5CxwQvCVb6-7BJS4oJW-Kyf0IHRDNSK87VXrljKWpBNd9Hhzk_YYwbItgXtE-5UJwqcYCm2zFNdpyS6SsTNtUqvPgUwwBhLMoFmDKCXEVX_Ywv0Fc_Nu6sujfJmzBuDVthDaFAPlQ3YOPQ-TBPb-DBx7D1rrJ9hOTtozeVjb3_ij4702c4ej0X6PfF6u78sr7-tb46P7uuLdNqrJXTXDODjW2E5sI5JxuihHBNeWhnlOuIMloKhaV0mqpOKgqCdtJQK6RlC_Rtl_uc4p8J8tgOPlvoexMgTrklxdpIKjn-GG2EIIRxRgp6-h_6FKcUykdmqilLY1WoekfZFHNO4Nrn5AeT_rYEt3N1bamunatr5-oKf_KaOnUDbN7pt64KwHbALJsQeg8dpPGD2H8k16Q4</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Wang, Dongguo</creator><creator>Liang, Haiyan</creator><creator>Chen, Jiayu</creator><creator>Mou, Yonghua</creator><creator>Qi, Yongxiao</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20141001</creationdate><title>Structural and Environmental Features of Novel MdfA Variant and MdfA Genes in Recombinant Regions of Escherichia coli</title><author>Wang, Dongguo ; Liang, Haiyan ; Chen, Jiayu ; Mou, Yonghua ; Qi, Yongxiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-8f9493a0ac56946fff751866f546f9fa8fb18a9768077f928b782e62b7a2c67c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Biological Transport</topic><topic>Chloramphenicol - pharmacology</topic><topic>Conjugation, Genetic</topic><topic>Deoxyribonuclease EcoRI - genetics</topic><topic>Deoxyribonuclease EcoRI - metabolism</topic><topic>Drug resistance</topic><topic>Drug Resistance, Multiple, Bacterial - drug effects</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>E coli</topic><topic>Erythromycin - pharmacology</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli Infections - drug therapy</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene mapping</topic><topic>Genetic recombination</topic><topic>Genotype</topic><topic>Humans</topic><topic>Mechanisms</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Plasmids</topic><topic>Plasmids - chemistry</topic><topic>Plasmids - metabolism</topic><topic>Serine-Type D-Ala-D-Ala Carboxypeptidase - genetics</topic><topic>Serine-Type D-Ala-D-Ala Carboxypeptidase - metabolism</topic><topic>Tetracycline - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Dongguo</creatorcontrib><creatorcontrib>Liang, Haiyan</creatorcontrib><creatorcontrib>Chen, Jiayu</creatorcontrib><creatorcontrib>Mou, Yonghua</creatorcontrib><creatorcontrib>Qi, Yongxiao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Dongguo</au><au>Liang, Haiyan</au><au>Chen, Jiayu</au><au>Mou, Yonghua</au><au>Qi, Yongxiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural and Environmental Features of Novel MdfA Variant and MdfA Genes in Recombinant Regions of Escherichia coli</atitle><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle><addtitle>Microb Drug Resist</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>20</volume><issue>5</issue><spage>392</spage><epage>398</epage><pages>392-398</pages><issn>1076-6294</issn><eissn>1931-8448</eissn><abstract>Novel
mdfA
gene variants were identified simultaneously from 3 of 13 positive isolates of PCR amplification in
Escherichia coli
from patients. These 13 positive isolates showed resistance to chloramphenicol, tetracycline, and erythromycin. The 3
mdfA
gene variants were of the same genotype and all the 13 positive isolates were investigated by conjugation experiment,
Eco
RI restriction, and gene mapping. Conjugation experiments demonstrated that the novel
mdfA
variant and
mdfA
genes were located on plasmids that were restricted by
Eco
RI for ∼8.2 kb-length, which was also validated by gene mapping. Further study indicated three types of genetic structures (A, B, and C) in the recombinant plasmids harboring
mdfA
and surrounding genes, and structure B was first reported in the article. Structure A comprises two partial-length and six full-length genes, including the
mdfA
gene variant in the recombinant plasmid; structure B comprises four full-length genes, the
mdfA
,
ybjG
,
dacC
, and
ybjI
; structure C comprises two full-length genes, the
mdfA
and
dacC
. These results suggested that the
mdfA
gene can function as transporter responsible for multidrug resistance and also mediated the synergistic function with its surrounding genes in conjugative plasmids.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>24684286</pmid><doi>10.1089/mdr.2013.0201</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1076-6294 |
| ispartof | Microbial drug resistance (Larchmont, N.Y.), 2014-10, Vol.20 (5), p.392-398 |
| issn | 1076-6294 1931-8448 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1768572740 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Anti-Bacterial Agents - pharmacology Antibiotics Biological Transport Chloramphenicol - pharmacology Conjugation, Genetic Deoxyribonuclease EcoRI - genetics Deoxyribonuclease EcoRI - metabolism Drug resistance Drug Resistance, Multiple, Bacterial - drug effects Drug Resistance, Multiple, Bacterial - genetics E coli Erythromycin - pharmacology Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli - metabolism Escherichia coli Infections - drug therapy Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Gene Expression Regulation, Bacterial Gene mapping Genetic recombination Genotype Humans Mechanisms Membrane Proteins - genetics Membrane Proteins - metabolism Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Plasmids Plasmids - chemistry Plasmids - metabolism Serine-Type D-Ala-D-Ala Carboxypeptidase - genetics Serine-Type D-Ala-D-Ala Carboxypeptidase - metabolism Tetracycline - pharmacology |
| title | Structural and Environmental Features of Novel MdfA Variant and MdfA Genes in Recombinant Regions of Escherichia coli |
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