Oxidative damage induced by copper in mouse primary hepatocytes by single-cell analysis
Copper can disturb the intracellular redox balance, induce oxidative stress, and subsequently cause irreversible damage, leading to a variety of diseases. In the present study, mouse primary hepatocytes were chosen to elucidate the in vitro oxidative damage of short-term copper exposure (10–200 μM)...
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| Veröffentlicht in: | Environmental science and pollution research international 2016-01, Vol.23 (2), p.1335-1343 |
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| creator | Jing, Mingyang Liu, Yang Song, Wei Yan, Yunxing Yan, Wenbao Liu, Rutao |
| description | Copper can disturb the intracellular redox balance, induce oxidative stress, and subsequently cause irreversible damage, leading to a variety of diseases. In the present study, mouse primary hepatocytes were chosen to elucidate the in vitro oxidative damage of short-term copper exposure (10–200 μM) by single-cell analysis. We evaluated the toxicity of copper by reactive oxygen species (ROS), glutathione (GSH), and oxidative DNA damage at the single-cell level. Oxidative damage induced by copper was verified by the morphological changes, persistent elevations of excessive ROS and malondialdehyde (MDA), a decrease in GSH level, and the oxidative DNA damage. Furthermore, the average ROS generation, GSH consumption, and the indicators in DNA damage did not significantly change at relatively low concentrations (10 or 50 μM), but we can find the alterations of parameters in some single cells clearly. Emphasis on the analysis of single cells is conducive to gain a better understanding on the toxicity of copper. This study will also complement studies on the environmental risk assessment of copper pollution. |
| doi_str_mv | 10.1007/s11356-015-5360-3 |
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In the present study, mouse primary hepatocytes were chosen to elucidate the in vitro oxidative damage of short-term copper exposure (10–200 μM) by single-cell analysis. We evaluated the toxicity of copper by reactive oxygen species (ROS), glutathione (GSH), and oxidative DNA damage at the single-cell level. Oxidative damage induced by copper was verified by the morphological changes, persistent elevations of excessive ROS and malondialdehyde (MDA), a decrease in GSH level, and the oxidative DNA damage. Furthermore, the average ROS generation, GSH consumption, and the indicators in DNA damage did not significantly change at relatively low concentrations (10 or 50 μM), but we can find the alterations of parameters in some single cells clearly. Emphasis on the analysis of single cells is conducive to gain a better understanding on the toxicity of copper. This study will also complement studies on the environmental risk assessment of copper pollution.</description><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-015-5360-3</identifier><identifier>PMID: 26358217</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Aquatic Pollution ; Atmospheric Protection/Air Quality Control/Air Pollution ; Cells, Cultured ; Copper ; Copper - toxicity ; Deoxyribonucleic acid ; DNA ; DNA damage ; DNA Damage - drug effects ; Earth and Environmental Science ; Ecotoxicology ; Environment ; Environmental assessment ; Environmental Chemistry ; Environmental Health ; Environmental risk ; Environmental studies ; Enzymes ; Glutathione ; Glutathione - metabolism ; Heavy metals ; Hepatocytes ; Hepatocytes - drug effects ; Hepatocytes - metabolism ; Humidity ; Laboratories ; Land pollution ; Liver ; malondialdehyde ; Malondialdehyde - metabolism ; Metals ; Mice ; Oxidative stress ; Oxidative Stress - drug effects ; Physiology ; Pollution ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Reagents ; Remediation ; Research Article ; Risk assessment ; Single-Cell Analysis ; Toxicity ; Toxicology ; Waste Water Technology ; Water Management ; Water Pollution Control</subject><ispartof>Environmental science and pollution research international, 2016-01, Vol.23 (2), p.1335-1343</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-5dd2262c285d9f1f93b24f739bcdd62fd1dc0431bf0fb1a426ee3cf1998326683</citedby><cites>FETCH-LOGICAL-c540t-5dd2262c285d9f1f93b24f739bcdd62fd1dc0431bf0fb1a426ee3cf1998326683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11356-015-5360-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11356-015-5360-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26358217$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jing, Mingyang</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Song, Wei</creatorcontrib><creatorcontrib>Yan, Yunxing</creatorcontrib><creatorcontrib>Yan, Wenbao</creatorcontrib><creatorcontrib>Liu, Rutao</creatorcontrib><title>Oxidative damage induced by copper in mouse primary hepatocytes by single-cell analysis</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>Copper can disturb the intracellular redox balance, induce oxidative stress, and subsequently cause irreversible damage, leading to a variety of diseases. In the present study, mouse primary hepatocytes were chosen to elucidate the in vitro oxidative damage of short-term copper exposure (10–200 μM) by single-cell analysis. We evaluated the toxicity of copper by reactive oxygen species (ROS), glutathione (GSH), and oxidative DNA damage at the single-cell level. Oxidative damage induced by copper was verified by the morphological changes, persistent elevations of excessive ROS and malondialdehyde (MDA), a decrease in GSH level, and the oxidative DNA damage. Furthermore, the average ROS generation, GSH consumption, and the indicators in DNA damage did not significantly change at relatively low concentrations (10 or 50 μM), but we can find the alterations of parameters in some single cells clearly. Emphasis on the analysis of single cells is conducive to gain a better understanding on the toxicity of copper. This study will also complement studies on the environmental risk assessment of copper pollution.</description><subject>Animals</subject><subject>Aquatic Pollution</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Cells, Cultured</subject><subject>Copper</subject><subject>Copper - toxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Environment</subject><subject>Environmental assessment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Environmental risk</subject><subject>Environmental studies</subject><subject>Enzymes</subject><subject>Glutathione</subject><subject>Glutathione - metabolism</subject><subject>Heavy metals</subject><subject>Hepatocytes</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - metabolism</subject><subject>Humidity</subject><subject>Laboratories</subject><subject>Land pollution</subject><subject>Liver</subject><subject>malondialdehyde</subject><subject>Malondialdehyde - metabolism</subject><subject>Metals</subject><subject>Mice</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Physiology</subject><subject>Pollution</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Reagents</subject><subject>Remediation</subject><subject>Research Article</subject><subject>Risk assessment</subject><subject>Single-Cell Analysis</subject><subject>Toxicity</subject><subject>Toxicology</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><issn>0944-1344</issn><issn>1614-7499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kEtv1TAQhS0EopeWH9ANjdQNG8OM7TjxElXlIVXqAqouLcePS6q8aicV99_jKJcKsWBlafydM2cOIecIHxCg-pgQeSkpYElLLoHyF2SHEgWthFIvyQ6UEBS5ECfkTUoPAAwUq16TEyZ5WTOsduT-9lfrzNw--cKZ3ux90Q5usd4VzaGw4zT5mCdFPy7JF1NsexMPxU8_mXm0h9mnFUvtsO88tb7rCjOY7pDadEZeBdMl__b4npK7z9c_rr7Sm9sv364-3VBbCphp6RxjkllWl04FDIo3TISKq8Y6J1lw6CwIjk2A0KARTHrPbUClas6krPkpeb_5TnF8XHyadd-mNYkZfM6ssZJ1WTFWQ0Yv_0EfxiXmvCtV1xK4QJEp3Cgbx5SiD_p4tUbQa-t6a13n1vXauuZZ8-7ovDS9d8-KPzVngG1Ayl_D3se_Vv_H9WITBTNqs49t0nffGaAEQI75Kv4bIWOVvQ</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Jing, Mingyang</creator><creator>Liu, Yang</creator><creator>Song, Wei</creator><creator>Yan, Yunxing</creator><creator>Yan, Wenbao</creator><creator>Liu, Rutao</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7TV</scope><scope>7U7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7ST</scope><scope>7TG</scope><scope>KL.</scope><scope>SOI</scope></search><sort><creationdate>20160101</creationdate><title>Oxidative damage induced by copper in mouse primary hepatocytes by single-cell analysis</title><author>Jing, Mingyang ; Liu, Yang ; Song, Wei ; Yan, Yunxing ; Yan, Wenbao ; Liu, Rutao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-5dd2262c285d9f1f93b24f739bcdd62fd1dc0431bf0fb1a426ee3cf1998326683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Aquatic Pollution</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Cells, Cultured</topic><topic>Copper</topic><topic>Copper - 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Academic</collection><collection>Environment Abstracts</collection><jtitle>Environmental science and pollution research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jing, Mingyang</au><au>Liu, Yang</au><au>Song, Wei</au><au>Yan, Yunxing</au><au>Yan, Wenbao</au><au>Liu, Rutao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oxidative damage induced by copper in mouse primary hepatocytes by single-cell analysis</atitle><jtitle>Environmental science and pollution research international</jtitle><stitle>Environ Sci Pollut Res</stitle><addtitle>Environ Sci Pollut Res Int</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>23</volume><issue>2</issue><spage>1335</spage><epage>1343</epage><pages>1335-1343</pages><issn>0944-1344</issn><eissn>1614-7499</eissn><abstract>Copper can disturb the intracellular redox balance, induce oxidative stress, and subsequently cause irreversible damage, leading to a variety of diseases. In the present study, mouse primary hepatocytes were chosen to elucidate the in vitro oxidative damage of short-term copper exposure (10–200 μM) by single-cell analysis. We evaluated the toxicity of copper by reactive oxygen species (ROS), glutathione (GSH), and oxidative DNA damage at the single-cell level. Oxidative damage induced by copper was verified by the morphological changes, persistent elevations of excessive ROS and malondialdehyde (MDA), a decrease in GSH level, and the oxidative DNA damage. Furthermore, the average ROS generation, GSH consumption, and the indicators in DNA damage did not significantly change at relatively low concentrations (10 or 50 μM), but we can find the alterations of parameters in some single cells clearly. Emphasis on the analysis of single cells is conducive to gain a better understanding on the toxicity of copper. This study will also complement studies on the environmental risk assessment of copper pollution.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26358217</pmid><doi>10.1007/s11356-015-5360-3</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution Cells, Cultured Copper Copper - toxicity Deoxyribonucleic acid DNA DNA damage DNA Damage - drug effects Earth and Environmental Science Ecotoxicology Environment Environmental assessment Environmental Chemistry Environmental Health Environmental risk Environmental studies Enzymes Glutathione Glutathione - metabolism Heavy metals Hepatocytes Hepatocytes - drug effects Hepatocytes - metabolism Humidity Laboratories Land pollution Liver malondialdehyde Malondialdehyde - metabolism Metals Mice Oxidative stress Oxidative Stress - drug effects Physiology Pollution Reactive oxygen species Reactive Oxygen Species - metabolism Reagents Remediation Research Article Risk assessment Single-Cell Analysis Toxicity Toxicology Waste Water Technology Water Management Water Pollution Control |
| title | Oxidative damage induced by copper in mouse primary hepatocytes by single-cell analysis |
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