Identification and topographical characterisation of microbial nanowires in Nostoc punctiforme
Extracellular pili-like structures (PLS) produced by cyanobacteria have been poorly explored. We have done detailed topographical and electrical characterisation of PLS in Nostoc punctiforme PCC 73120 using transmission electron microscopy (TEM) and conductive atomic force microscopy (CAFM). TEM ana...
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| Veröffentlicht in: | Antonie van Leeuwenhoek 2016-03, Vol.109 (3), p.475-480 |
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| description | Extracellular pili-like structures (PLS) produced by cyanobacteria have been poorly explored. We have done detailed topographical and electrical characterisation of PLS in Nostoc punctiforme PCC 73120 using transmission electron microscopy (TEM) and conductive atomic force microscopy (CAFM). TEM analysis showed that N. punctiforme produces two separate types of PLS differing in their length and diameter. The first type of PLS are 6–7.5 nm in diameter and 0.5–2 µm in length (short/thin PLS) while the second type of PLS are ~20–40 nm in diameter and more than 10 µm long (long/thick PLS). This is the first study to report long/thick PLS in N. punctiforme. Electrical characterisation of these two different PLS by CAFM showed that both are electrically conductive and can act as microbial nanowires. This is the first report to show two distinct PLS and also identifies microbial nanowires in N. punctiforme. This study paves the way for more detailed investigation of N. punctiforme nanowires and their potential role in cell physiology and symbiosis with plants. |
| doi_str_mv | 10.1007/s10482-015-0644-7 |
| format | Article |
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J ; Gaur, Aditya ; Li, Lu Hua ; Chen, Ying ; Tripathi, Chandrakant ; Adholeya, Alok ; Ackland, M. Leigh ; Kochar, Mandira</creator><creatorcontrib>Sure, Sandeep ; Torriero, Angel A. J ; Gaur, Aditya ; Li, Lu Hua ; Chen, Ying ; Tripathi, Chandrakant ; Adholeya, Alok ; Ackland, M. Leigh ; Kochar, Mandira</creatorcontrib><description>Extracellular pili-like structures (PLS) produced by cyanobacteria have been poorly explored. We have done detailed topographical and electrical characterisation of PLS in Nostoc punctiforme PCC 73120 using transmission electron microscopy (TEM) and conductive atomic force microscopy (CAFM). TEM analysis showed that N. punctiforme produces two separate types of PLS differing in their length and diameter. The first type of PLS are 6–7.5 nm in diameter and 0.5–2 µm in length (short/thin PLS) while the second type of PLS are ~20–40 nm in diameter and more than 10 µm long (long/thick PLS). This is the first study to report long/thick PLS in N. punctiforme. Electrical characterisation of these two different PLS by CAFM showed that both are electrically conductive and can act as microbial nanowires. This is the first report to show two distinct PLS and also identifies microbial nanowires in N. punctiforme. This study paves the way for more detailed investigation of N. punctiforme nanowires and their potential role in cell physiology and symbiosis with plants.</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-015-0644-7</identifier><identifier>PMID: 26754102</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>atomic force microscopy ; Biomedical and Life Sciences ; cell physiology ; Cyanobacteria ; Life Sciences ; Medical Microbiology ; Microbiology ; Nanowires ; Nanowires - ultrastructure ; Nostoc - physiology ; Nostoc - ultrastructure ; Nostoc punctiforme ; Plant Sciences ; Short Communication ; Soil microorganisms ; Soil Science & Conservation ; Symbiosis ; Topography ; Transmission electron microscopy</subject><ispartof>Antonie van Leeuwenhoek, 2016-03, Vol.109 (3), p.475-480</ispartof><rights>Springer International Publishing Switzerland 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-1a84303885ed1a79a8fae028e57961c218635d43663f005693f9eebd220b524b3</citedby><cites>FETCH-LOGICAL-c466t-1a84303885ed1a79a8fae028e57961c218635d43663f005693f9eebd220b524b3</cites><orcidid>0000-0002-8835-9629</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10482-015-0644-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10482-015-0644-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26754102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sure, Sandeep</creatorcontrib><creatorcontrib>Torriero, Angel A. J</creatorcontrib><creatorcontrib>Gaur, Aditya</creatorcontrib><creatorcontrib>Li, Lu Hua</creatorcontrib><creatorcontrib>Chen, Ying</creatorcontrib><creatorcontrib>Tripathi, Chandrakant</creatorcontrib><creatorcontrib>Adholeya, Alok</creatorcontrib><creatorcontrib>Ackland, M. Leigh</creatorcontrib><creatorcontrib>Kochar, Mandira</creatorcontrib><title>Identification and topographical characterisation of microbial nanowires in Nostoc punctiforme</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><description>Extracellular pili-like structures (PLS) produced by cyanobacteria have been poorly explored. We have done detailed topographical and electrical characterisation of PLS in Nostoc punctiforme PCC 73120 using transmission electron microscopy (TEM) and conductive atomic force microscopy (CAFM). TEM analysis showed that N. punctiforme produces two separate types of PLS differing in their length and diameter. The first type of PLS are 6–7.5 nm in diameter and 0.5–2 µm in length (short/thin PLS) while the second type of PLS are ~20–40 nm in diameter and more than 10 µm long (long/thick PLS). This is the first study to report long/thick PLS in N. punctiforme. Electrical characterisation of these two different PLS by CAFM showed that both are electrically conductive and can act as microbial nanowires. This is the first report to show two distinct PLS and also identifies microbial nanowires in N. punctiforme. This study paves the way for more detailed investigation of N. punctiforme nanowires and their potential role in cell physiology and symbiosis with plants.</description><subject>atomic force microscopy</subject><subject>Biomedical and Life Sciences</subject><subject>cell physiology</subject><subject>Cyanobacteria</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Nanowires</subject><subject>Nanowires - ultrastructure</subject><subject>Nostoc - physiology</subject><subject>Nostoc - ultrastructure</subject><subject>Nostoc punctiforme</subject><subject>Plant Sciences</subject><subject>Short Communication</subject><subject>Soil microorganisms</subject><subject>Soil Science & Conservation</subject><subject>Symbiosis</subject><subject>Topography</subject><subject>Transmission electron microscopy</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1O3DAURq2KqgyUB-gGInXTTcr1v7NEI1qQEF3Q2WI5jjMYTexgJ0K8fT0KrSoWrCz7nvvZPhehLxi-YwB5njEwRWrAvAbBWC0_oBXmktSNaJoDtAIAWguQ5BAd5fxYto1Q8hM6JEJyhoGs0P1158Lke2_N5GOoTOiqKY5xm8z4UA53lX0wydjJJZ8XJPbV4G2KrS_VYEJ89snlyofqNuYp2mqcgy2RMQ3uM_rYm112J6_rMdr8uPy9vqpvfv28Xl_c1JYJMdXYKEaBKsVdh41sjOqNA6Icl43AlmAlKO8YFYL2AFw0tG-caztCoOWEtfQYfVtyxxSfZpcnPfhs3W5ngotz1lgKxQVhVBX06xv0Mc4plNcVSmJcdDFaKLxQ5aM5J9frMfnBpBeNQe_l60W-LvL1Xr6Wpef0NXluB9f96_hruwBkAXIpha1L_139TurZ0tSbqM22jEFv7ghgUcapBOFA_wBJE5gg</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Sure, Sandeep</creator><creator>Torriero, Angel A. 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J</au><au>Gaur, Aditya</au><au>Li, Lu Hua</au><au>Chen, Ying</au><au>Tripathi, Chandrakant</au><au>Adholeya, Alok</au><au>Ackland, M. Leigh</au><au>Kochar, Mandira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and topographical characterisation of microbial nanowires in Nostoc punctiforme</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><stitle>Antonie van Leeuwenhoek</stitle><addtitle>Antonie Van Leeuwenhoek</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>109</volume><issue>3</issue><spage>475</spage><epage>480</epage><pages>475-480</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><abstract>Extracellular pili-like structures (PLS) produced by cyanobacteria have been poorly explored. We have done detailed topographical and electrical characterisation of PLS in Nostoc punctiforme PCC 73120 using transmission electron microscopy (TEM) and conductive atomic force microscopy (CAFM). TEM analysis showed that N. punctiforme produces two separate types of PLS differing in their length and diameter. The first type of PLS are 6–7.5 nm in diameter and 0.5–2 µm in length (short/thin PLS) while the second type of PLS are ~20–40 nm in diameter and more than 10 µm long (long/thick PLS). This is the first study to report long/thick PLS in N. punctiforme. Electrical characterisation of these two different PLS by CAFM showed that both are electrically conductive and can act as microbial nanowires. This is the first report to show two distinct PLS and also identifies microbial nanowires in N. punctiforme. This study paves the way for more detailed investigation of N. punctiforme nanowires and their potential role in cell physiology and symbiosis with plants.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>26754102</pmid><doi>10.1007/s10482-015-0644-7</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-8835-9629</orcidid></addata></record> |
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| subjects | atomic force microscopy Biomedical and Life Sciences cell physiology Cyanobacteria Life Sciences Medical Microbiology Microbiology Nanowires Nanowires - ultrastructure Nostoc - physiology Nostoc - ultrastructure Nostoc punctiforme Plant Sciences Short Communication Soil microorganisms Soil Science & Conservation Symbiosis Topography Transmission electron microscopy |
| title | Identification and topographical characterisation of microbial nanowires in Nostoc punctiforme |
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