Effect of amino acid substitution at the P sub(3) and P sub(4) subsites of fusion proteins on Kex2 protease activity

The effect of amino acid substitution at the P sub(3) and P sub(4) subsites on the cleavage activity of a recombinant secretory-type Kex2 protease (Kex2-660) was investigated using recombinant fusion proteins. They were constructed from a truncated Escherichia coli beta -galactosidase ( beta G-117S)...

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Veröffentlicht in:	Biotechnology and applied biochemistry 2000-08, Vol.32 (1), p.53-60
Hauptverfasser:	Suzuki, Y, Ikeda, N, Kataoka, E, Ohsuye, K
Format:	Artikel
Sprache:	eng
Schlagworte:	amino acids Kex2 protein
Online-Zugang:	Volltext
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