Cytogenetic Damage in Circulating Lymphocytes and Buccal Mucosa Cells of Head-and-neck Cancer Patients Undergoing Radiotherapy
This study evaluated cytogenetic damage by measuring the frequency of micronucleated cells (MNC) in peripheral blood and buccal mucosa of head-and-neck cancer patients undergoing radiotherapy. MNC frequencies were assessed in 31 patients before, during, and after radiotherapy, and in 17 healthy cont...
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| Veröffentlicht in: | JOURNAL OF RADIATION RESEARCH 2005-06, Vol.46 (2), p.135-142 |
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| creator | Minicucci, Eliana Maria Kowalski, Luis Paulo Maia, Maria Aparecida Conte Pereira, Adelino Ribeiro, Lúcia Regina de Camargo, João Lauro Viana Salvadori, Daisy Maria Favero |
| description | This study evaluated cytogenetic damage by measuring the frequency of micronucleated cells (MNC) in peripheral blood and buccal mucosa of head-and-neck cancer patients undergoing radiotherapy. MNC frequencies were assessed in 31 patients before, during, and after radiotherapy, and in 17 healthy controls matched for gender, age, and smoking habits. Results showed no statistically significant difference between patients and controls prior to radiotherapy in cytokinesis-blocked lymphocytes or buccal mucosa cells. During treatment, increased MNC frequencies were observed in both cell types. Micronucleated lymphocyte levels remained high in samples collected 30 to 140 days after the end of treatment, while MNC frequency in buccal mucosa decreased to values statistically similar to baseline values. There is controversy over the effects of age, smoking habit, tumor stage, and/or metastasis on MNC frequency. However, increased frequency of micronucleated buccal mucosa cells was seen in patients under 60 years old and in those with tumors >4cm. In conclusion, the data show that radiotherapy has a potent clastogenic effect in circulating lymphocytes and buccal mucosa cells of head-and-neck cancer patients, and that the baseline MNC frequency in these two tissues is not a sensitive marker for head-and neck neoplasm. |
| doi_str_mv | 10.1269/jrr.46.135 |
| format | Article |
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C. Camargo ; Sao Paulo - SP - Brazil ; Departamento de Dermatologia - Faculdade de Medicina - UNESP ; Botucatu - SP - Brazil ; Departamento de Patologia ; Nucleo de Avaliacao Toxicogenetica e Cancerigena - TOXICAN</creatorcontrib><description>This study evaluated cytogenetic damage by measuring the frequency of micronucleated cells (MNC) in peripheral blood and buccal mucosa of head-and-neck cancer patients undergoing radiotherapy. MNC frequencies were assessed in 31 patients before, during, and after radiotherapy, and in 17 healthy controls matched for gender, age, and smoking habits. Results showed no statistically significant difference between patients and controls prior to radiotherapy in cytokinesis-blocked lymphocytes or buccal mucosa cells. During treatment, increased MNC frequencies were observed in both cell types. Micronucleated lymphocyte levels remained high in samples collected 30 to 140 days after the end of treatment, while MNC frequency in buccal mucosa decreased to values statistically similar to baseline values. There is controversy over the effects of age, smoking habit, tumor stage, and/or metastasis on MNC frequency. However, increased frequency of micronucleated buccal mucosa cells was seen in patients under 60 years old and in those with tumors >4cm. In conclusion, the data show that radiotherapy has a potent clastogenic effect in circulating lymphocytes and buccal mucosa cells of head-and-neck cancer patients, and that the baseline MNC frequency in these two tissues is not a sensitive marker for head-and neck neoplasm.</description><identifier>ISSN: 0449-3060</identifier><identifier>EISSN: 1349-9157</identifier><identifier>DOI: 10.1269/jrr.46.135</identifier><identifier>PMID: 15988130</identifier><language>eng</language><publisher>England: THE JAPAN RADIATION RESEARCH SOCIETY</publisher><subject>Adult ; Age Distribution ; Aged ; Chromosome Aberrations - statistics & numerical data ; Comorbidity ; Female ; Head and Neck Neoplasms - blood ; Head and Neck Neoplasms - epidemiology ; Head and Neck Neoplasms - genetics ; Head and Neck Neoplasms - radiotherapy ; Humans ; Lymphocytes - radiation effects ; Male ; Micronucleus Tests ; Middle Aged ; Mouth Mucosa - radiation effects ; Prevalence ; Radiation Injuries - blood ; Radiation Injuries - epidemiology ; Radiation Injuries - genetics ; Radiation Protection - methods ; Radiotherapy - statistics & numerical data ; Risk Assessment - methods ; Risk Factors ; Smoking - epidemiology ; Treatment Outcome</subject><ispartof>JOURNAL OF RADIATION RESEARCH, 2005-06, Vol.46 (2), p.135-142</ispartof><rights>Copyright Japan Science and Technology Agency 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-2619ee6b82009cfc280defaa15cc746ea7f48b3b792eb0d37a8de013518813c53</citedby><cites>FETCH-LOGICAL-c558t-2619ee6b82009cfc280defaa15cc746ea7f48b3b792eb0d37a8de013518813c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15988130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Minicucci, Eliana Maria</creatorcontrib><creatorcontrib>Kowalski, Luis Paulo</creatorcontrib><creatorcontrib>Maia, Maria Aparecida Conte</creatorcontrib><creatorcontrib>Pereira, Adelino</creatorcontrib><creatorcontrib>Ribeiro, Lúcia Regina</creatorcontrib><creatorcontrib>de Camargo, João Lauro Viana</creatorcontrib><creatorcontrib>Salvadori, Daisy Maria Favero</creatorcontrib><creatorcontrib>Faculdade de Medicina - UNESP</creatorcontrib><creatorcontrib>Hospital A. C. Camargo</creatorcontrib><creatorcontrib>Sao Paulo - SP - Brazil</creatorcontrib><creatorcontrib>Departamento de Dermatologia - Faculdade de Medicina - UNESP</creatorcontrib><creatorcontrib>Botucatu - SP - Brazil</creatorcontrib><creatorcontrib>Departamento de Patologia</creatorcontrib><creatorcontrib>Nucleo de Avaliacao Toxicogenetica e Cancerigena - TOXICAN</creatorcontrib><title>Cytogenetic Damage in Circulating Lymphocytes and Buccal Mucosa Cells of Head-and-neck Cancer Patients Undergoing Radiotherapy</title><title>JOURNAL OF RADIATION RESEARCH</title><addtitle>J Radiat Res</addtitle><description>This study evaluated cytogenetic damage by measuring the frequency of micronucleated cells (MNC) in peripheral blood and buccal mucosa of head-and-neck cancer patients undergoing radiotherapy. MNC frequencies were assessed in 31 patients before, during, and after radiotherapy, and in 17 healthy controls matched for gender, age, and smoking habits. Results showed no statistically significant difference between patients and controls prior to radiotherapy in cytokinesis-blocked lymphocytes or buccal mucosa cells. During treatment, increased MNC frequencies were observed in both cell types. Micronucleated lymphocyte levels remained high in samples collected 30 to 140 days after the end of treatment, while MNC frequency in buccal mucosa decreased to values statistically similar to baseline values. There is controversy over the effects of age, smoking habit, tumor stage, and/or metastasis on MNC frequency. However, increased frequency of micronucleated buccal mucosa cells was seen in patients under 60 years old and in those with tumors >4cm. In conclusion, the data show that radiotherapy has a potent clastogenic effect in circulating lymphocytes and buccal mucosa cells of head-and-neck cancer patients, and that the baseline MNC frequency in these two tissues is not a sensitive marker for head-and neck neoplasm.</description><subject>Adult</subject><subject>Age Distribution</subject><subject>Aged</subject><subject>Chromosome Aberrations - statistics & numerical data</subject><subject>Comorbidity</subject><subject>Female</subject><subject>Head and Neck Neoplasms - blood</subject><subject>Head and Neck Neoplasms - epidemiology</subject><subject>Head and Neck Neoplasms - genetics</subject><subject>Head and Neck Neoplasms - radiotherapy</subject><subject>Humans</subject><subject>Lymphocytes - radiation effects</subject><subject>Male</subject><subject>Micronucleus Tests</subject><subject>Middle Aged</subject><subject>Mouth Mucosa - radiation effects</subject><subject>Prevalence</subject><subject>Radiation Injuries - blood</subject><subject>Radiation Injuries - epidemiology</subject><subject>Radiation Injuries - genetics</subject><subject>Radiation Protection - methods</subject><subject>Radiotherapy - statistics & numerical data</subject><subject>Risk Assessment - methods</subject><subject>Risk Factors</subject><subject>Smoking - epidemiology</subject><subject>Treatment Outcome</subject><issn>0449-3060</issn><issn>1349-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkdGL1DAQxoMo3nLei3-ABAQfhK6TtkmTR62eJ6wo4j2HNJ3uZa9N1qR96It_u1l24cCXTGB-fPN9M4S8ZrBlpVAfDjFua7FlFX9GNqyqVaEYb56TDdT5X4GAK3KTkuuAcQEgGXtJrhhXUrIKNuRvu85hjx5nZ-lnM5k9Uudp66JdRjM7v6e7dTo-BLvOmKjxPf20WGtG-n2xIRna4jgmGgZ6h6Yvcr_waB9pa7zFSH9mCfRzove-x7gPJ71fpndhfsBojusr8mIwY8KbS70m97dffrd3xe7H12_tx11hOZdzUQqmEEUnSwBlB1tK6HEwhnFrm1qgaYZadlXXqBI76KvGyB4h74SdYlpeXZN3Z91jDH8WTLOeXLLZuvEYlqRZI4RUDWTw7X_gISzRZ2-a1XmjlQQlMvX-TNkYUoo46GN0k4mrZqBPZ9H5LLoWOlvI8JuL5NJN2D-hlyNk4PYM5K7Lqw1-dB6fBttHcQgxos7puQaoBZS5MH1KmJ-6ZI1iUFb_ABvPnuY</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Minicucci, Eliana Maria</creator><creator>Kowalski, Luis Paulo</creator><creator>Maia, Maria Aparecida Conte</creator><creator>Pereira, Adelino</creator><creator>Ribeiro, Lúcia Regina</creator><creator>de Camargo, João Lauro Viana</creator><creator>Salvadori, Daisy Maria Favero</creator><general>THE JAPAN RADIATION RESEARCH SOCIETY</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20050601</creationdate><title>Cytogenetic Damage in Circulating Lymphocytes and Buccal Mucosa Cells of Head-and-neck Cancer Patients Undergoing Radiotherapy</title><author>Minicucci, Eliana Maria ; Kowalski, Luis Paulo ; Maia, Maria Aparecida Conte ; Pereira, Adelino ; Ribeiro, Lúcia Regina ; de Camargo, João Lauro Viana ; Salvadori, Daisy Maria Favero</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-2619ee6b82009cfc280defaa15cc746ea7f48b3b792eb0d37a8de013518813c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adult</topic><topic>Age Distribution</topic><topic>Aged</topic><topic>Chromosome Aberrations - statistics & numerical data</topic><topic>Comorbidity</topic><topic>Female</topic><topic>Head and Neck Neoplasms - blood</topic><topic>Head and Neck Neoplasms - epidemiology</topic><topic>Head and Neck Neoplasms - genetics</topic><topic>Head and Neck Neoplasms - radiotherapy</topic><topic>Humans</topic><topic>Lymphocytes - radiation effects</topic><topic>Male</topic><topic>Micronucleus Tests</topic><topic>Middle Aged</topic><topic>Mouth Mucosa - radiation effects</topic><topic>Prevalence</topic><topic>Radiation Injuries - blood</topic><topic>Radiation Injuries - epidemiology</topic><topic>Radiation Injuries - genetics</topic><topic>Radiation Protection - methods</topic><topic>Radiotherapy - statistics & numerical data</topic><topic>Risk Assessment - methods</topic><topic>Risk Factors</topic><topic>Smoking - epidemiology</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Minicucci, Eliana Maria</creatorcontrib><creatorcontrib>Kowalski, Luis Paulo</creatorcontrib><creatorcontrib>Maia, Maria Aparecida Conte</creatorcontrib><creatorcontrib>Pereira, Adelino</creatorcontrib><creatorcontrib>Ribeiro, Lúcia Regina</creatorcontrib><creatorcontrib>de Camargo, João Lauro Viana</creatorcontrib><creatorcontrib>Salvadori, Daisy Maria Favero</creatorcontrib><creatorcontrib>Faculdade de Medicina - UNESP</creatorcontrib><creatorcontrib>Hospital A. C. Camargo</creatorcontrib><creatorcontrib>Sao Paulo - SP - Brazil</creatorcontrib><creatorcontrib>Departamento de Dermatologia - Faculdade de Medicina - UNESP</creatorcontrib><creatorcontrib>Botucatu - SP - Brazil</creatorcontrib><creatorcontrib>Departamento de Patologia</creatorcontrib><creatorcontrib>Nucleo de Avaliacao Toxicogenetica e Cancerigena - TOXICAN</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>JOURNAL OF RADIATION RESEARCH</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Minicucci, Eliana Maria</au><au>Kowalski, Luis Paulo</au><au>Maia, Maria Aparecida Conte</au><au>Pereira, Adelino</au><au>Ribeiro, Lúcia Regina</au><au>de Camargo, João Lauro Viana</au><au>Salvadori, Daisy Maria Favero</au><aucorp>Faculdade de Medicina - UNESP</aucorp><aucorp>Hospital A. C. Camargo</aucorp><aucorp>Sao Paulo - SP - Brazil</aucorp><aucorp>Departamento de Dermatologia - Faculdade de Medicina - UNESP</aucorp><aucorp>Botucatu - SP - Brazil</aucorp><aucorp>Departamento de Patologia</aucorp><aucorp>Nucleo de Avaliacao Toxicogenetica e Cancerigena - TOXICAN</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytogenetic Damage in Circulating Lymphocytes and Buccal Mucosa Cells of Head-and-neck Cancer Patients Undergoing Radiotherapy</atitle><jtitle>JOURNAL OF RADIATION RESEARCH</jtitle><addtitle>J Radiat Res</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>46</volume><issue>2</issue><spage>135</spage><epage>142</epage><pages>135-142</pages><issn>0449-3060</issn><eissn>1349-9157</eissn><abstract>This study evaluated cytogenetic damage by measuring the frequency of micronucleated cells (MNC) in peripheral blood and buccal mucosa of head-and-neck cancer patients undergoing radiotherapy. MNC frequencies were assessed in 31 patients before, during, and after radiotherapy, and in 17 healthy controls matched for gender, age, and smoking habits. Results showed no statistically significant difference between patients and controls prior to radiotherapy in cytokinesis-blocked lymphocytes or buccal mucosa cells. During treatment, increased MNC frequencies were observed in both cell types. Micronucleated lymphocyte levels remained high in samples collected 30 to 140 days after the end of treatment, while MNC frequency in buccal mucosa decreased to values statistically similar to baseline values. There is controversy over the effects of age, smoking habit, tumor stage, and/or metastasis on MNC frequency. However, increased frequency of micronucleated buccal mucosa cells was seen in patients under 60 years old and in those with tumors >4cm. In conclusion, the data show that radiotherapy has a potent clastogenic effect in circulating lymphocytes and buccal mucosa cells of head-and-neck cancer patients, and that the baseline MNC frequency in these two tissues is not a sensitive marker for head-and neck neoplasm.</abstract><cop>England</cop><pub>THE JAPAN RADIATION RESEARCH SOCIETY</pub><pmid>15988130</pmid><doi>10.1269/jrr.46.135</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Age Distribution Aged Chromosome Aberrations - statistics & numerical data Comorbidity Female Head and Neck Neoplasms - blood Head and Neck Neoplasms - epidemiology Head and Neck Neoplasms - genetics Head and Neck Neoplasms - radiotherapy Humans Lymphocytes - radiation effects Male Micronucleus Tests Middle Aged Mouth Mucosa - radiation effects Prevalence Radiation Injuries - blood Radiation Injuries - epidemiology Radiation Injuries - genetics Radiation Protection - methods Radiotherapy - statistics & numerical data Risk Assessment - methods Risk Factors Smoking - epidemiology Treatment Outcome |
| title | Cytogenetic Damage in Circulating Lymphocytes and Buccal Mucosa Cells of Head-and-neck Cancer Patients Undergoing Radiotherapy |
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