A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin

A chemically defined protein free medium, DF6S, was developed for the cultivation of a recombinant Chinese hamster ovary cell line (CHO2DS) producing human prothrombin in suspension batch culture. DF6S was formulated by optimizing DME/F12 with amino acids and supplementing the optimized DME/F12 with...

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Veröffentlicht in:	Biotechnology letters 2000, Vol.22 (10), p.837-841
Hauptverfasser:	ZHAOLIE CHEN, KAI IDING, LÜTKEMEYER, D, LEHMANN, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids aurintricarboxylic acid Biological and medical sciences Biotechnology Cells Coagulation factors ethanolamine ferric sulfate Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Production of active biomolecules prothrombin putrescine Rodents sodium pyruvate Sulfates
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