A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin
A chemically defined protein free medium, DF6S, was developed for the cultivation of a recombinant Chinese hamster ovary cell line (CHO2DS) producing human prothrombin in suspension batch culture. DF6S was formulated by optimizing DME/F12 with amino acids and supplementing the optimized DME/F12 with...
Gespeichert in:
| Veröffentlicht in: | Biotechnology letters 2000, Vol.22 (10), p.837-841 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 841 |
|---|---|
| container_issue | 10 |
| container_start_page | 837 |
| container_title | Biotechnology letters |
| container_volume | 22 |
| creator | ZHAOLIE CHEN KAI IDING LÜTKEMEYER, D LEHMANN, J |
| description | A chemically defined protein free medium, DF6S, was developed for the cultivation of a recombinant Chinese hamster ovary cell line (CHO2DS) producing human prothrombin in suspension batch culture. DF6S was formulated by optimizing DME/F12 with amino acids and supplementing the optimized DME/F12 with aurintricarboxylic acid, ethanolamine, ferric sulfate, Pluronic F68, putrescine and sodium pyruvate. From a seeding density of 2.3 × 10^sup 5^ cells ml^sup -1^, CHO2DS cells grown in suspension in DF6S medium reached a maximal cell density of 1.92 × 10^sup 6^ cells ml^sup -1^ with an accumulated prothrombin concentration of 16.7 mg l^sup -1^ after 6 days in culture.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1023/a:1005665530028 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_proquest_miscellaneous_17662565</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17662565</sourcerecordid><originalsourceid>FETCH-LOGICAL-c390t-48ac839c06b575b9e8fa3facde9d932ca175dd97f27e10b1fb6baf45cf5043673</originalsourceid><addsrcrecordid>eNpd0DFPwzAQBWALgUQpzKwWQmwBO47tmK2qCkWq1AXmyLHPravEKXYi1H9PWjox3fK9u6dD6J6SZ0py9qJfKSFcCM4ZIXl5gSaUS5YJKcUlmhBa0IwXKr9GNyntCCFKEjlBmxluup_MdKnHZgutN7ppDtiC8wEs3seuBx-wiwC4BeuHFrsuYo0jmK6tfdChx_PlGhtoGtyMoWPGDsaHzSm9jSd2i66cbhLcnecUfb0tPufLbLV-_5jPVplhivRZUWpTMmWIqLnktYLSaea0saCsYrnRVHJrlXS5BEpq6mpRa1dw4zgpmJBsip7-9o63vwdIfdX6dOymA3RDqqgUIueCj_DhH9x1Qwxjt0qyoqSi5GxEj2ek0_gYF3UwPlX76FsdDxUtcjFK9gtENHO5</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>734816853</pqid></control><display><type>article</type><title>A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin</title><source>SpringerLink Journals - AutoHoldings</source><creator>ZHAOLIE CHEN ; KAI IDING ; LÜTKEMEYER, D ; LEHMANN, J</creator><creatorcontrib>ZHAOLIE CHEN ; KAI IDING ; LÜTKEMEYER, D ; LEHMANN, J</creatorcontrib><description>A chemically defined protein free medium, DF6S, was developed for the cultivation of a recombinant Chinese hamster ovary cell line (CHO2DS) producing human prothrombin in suspension batch culture. DF6S was formulated by optimizing DME/F12 with amino acids and supplementing the optimized DME/F12 with aurintricarboxylic acid, ethanolamine, ferric sulfate, Pluronic F68, putrescine and sodium pyruvate. From a seeding density of 2.3 × 10^sup 5^ cells ml^sup -1^, CHO2DS cells grown in suspension in DF6S medium reached a maximal cell density of 1.92 × 10^sup 6^ cells ml^sup -1^ with an accumulated prothrombin concentration of 16.7 mg l^sup -1^ after 6 days in culture.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1023/a:1005665530028</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Amino acids ; aurintricarboxylic acid ; Biological and medical sciences ; Biotechnology ; Cells ; Coagulation factors ; ethanolamine ; ferric sulfate ; Fundamental and applied biological sciences. Psychology ; Health. Pharmaceutical industry ; Industrial applications and implications. Economical aspects ; Production of active biomolecules ; prothrombin ; putrescine ; Rodents ; sodium pyruvate ; Sulfates</subject><ispartof>Biotechnology letters, 2000, Vol.22 (10), p.837-841</ispartof><rights>2000 INIST-CNRS</rights><rights>Kluwer Academic Publishers 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-48ac839c06b575b9e8fa3facde9d932ca175dd97f27e10b1fb6baf45cf5043673</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27922,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1426816$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>ZHAOLIE CHEN</creatorcontrib><creatorcontrib>KAI IDING</creatorcontrib><creatorcontrib>LÜTKEMEYER, D</creatorcontrib><creatorcontrib>LEHMANN, J</creatorcontrib><title>A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin</title><title>Biotechnology letters</title><description>A chemically defined protein free medium, DF6S, was developed for the cultivation of a recombinant Chinese hamster ovary cell line (CHO2DS) producing human prothrombin in suspension batch culture. DF6S was formulated by optimizing DME/F12 with amino acids and supplementing the optimized DME/F12 with aurintricarboxylic acid, ethanolamine, ferric sulfate, Pluronic F68, putrescine and sodium pyruvate. From a seeding density of 2.3 × 10^sup 5^ cells ml^sup -1^, CHO2DS cells grown in suspension in DF6S medium reached a maximal cell density of 1.92 × 10^sup 6^ cells ml^sup -1^ with an accumulated prothrombin concentration of 16.7 mg l^sup -1^ after 6 days in culture.[PUBLICATION ABSTRACT]</description><subject>Amino acids</subject><subject>aurintricarboxylic acid</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cells</subject><subject>Coagulation factors</subject><subject>ethanolamine</subject><subject>ferric sulfate</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. Pharmaceutical industry</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Production of active biomolecules</subject><subject>prothrombin</subject><subject>putrescine</subject><subject>Rodents</subject><subject>sodium pyruvate</subject><subject>Sulfates</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0DFPwzAQBWALgUQpzKwWQmwBO47tmK2qCkWq1AXmyLHPravEKXYi1H9PWjox3fK9u6dD6J6SZ0py9qJfKSFcCM4ZIXl5gSaUS5YJKcUlmhBa0IwXKr9GNyntCCFKEjlBmxluup_MdKnHZgutN7ppDtiC8wEs3seuBx-wiwC4BeuHFrsuYo0jmK6tfdChx_PlGhtoGtyMoWPGDsaHzSm9jSd2i66cbhLcnecUfb0tPufLbLV-_5jPVplhivRZUWpTMmWIqLnktYLSaea0saCsYrnRVHJrlXS5BEpq6mpRa1dw4zgpmJBsip7-9o63vwdIfdX6dOymA3RDqqgUIueCj_DhH9x1Qwxjt0qyoqSi5GxEj2ek0_gYF3UwPlX76FsdDxUtcjFK9gtENHO5</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>ZHAOLIE CHEN</creator><creator>KAI IDING</creator><creator>LÜTKEMEYER, D</creator><creator>LEHMANN, J</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7QO</scope></search><sort><creationdate>2000</creationdate><title>A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin</title><author>ZHAOLIE CHEN ; KAI IDING ; LÜTKEMEYER, D ; LEHMANN, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-48ac839c06b575b9e8fa3facde9d932ca175dd97f27e10b1fb6baf45cf5043673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino acids</topic><topic>aurintricarboxylic acid</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cells</topic><topic>Coagulation factors</topic><topic>ethanolamine</topic><topic>ferric sulfate</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Production of active biomolecules</topic><topic>prothrombin</topic><topic>putrescine</topic><topic>Rodents</topic><topic>sodium pyruvate</topic><topic>Sulfates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ZHAOLIE CHEN</creatorcontrib><creatorcontrib>KAI IDING</creatorcontrib><creatorcontrib>LÜTKEMEYER, D</creatorcontrib><creatorcontrib>LEHMANN, J</creatorcontrib><collection>Pascal-Francis</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ZHAOLIE CHEN</au><au>KAI IDING</au><au>LÜTKEMEYER, D</au><au>LEHMANN, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin</atitle><jtitle>Biotechnology letters</jtitle><date>2000</date><risdate>2000</risdate><volume>22</volume><issue>10</issue><spage>837</spage><epage>841</epage><pages>837-841</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>A chemically defined protein free medium, DF6S, was developed for the cultivation of a recombinant Chinese hamster ovary cell line (CHO2DS) producing human prothrombin in suspension batch culture. DF6S was formulated by optimizing DME/F12 with amino acids and supplementing the optimized DME/F12 with aurintricarboxylic acid, ethanolamine, ferric sulfate, Pluronic F68, putrescine and sodium pyruvate. From a seeding density of 2.3 × 10^sup 5^ cells ml^sup -1^, CHO2DS cells grown in suspension in DF6S medium reached a maximal cell density of 1.92 × 10^sup 6^ cells ml^sup -1^ with an accumulated prothrombin concentration of 16.7 mg l^sup -1^ after 6 days in culture.[PUBLICATION ABSTRACT]</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1023/a:1005665530028</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2000, Vol.22 (10), p.837-841 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17662565 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Amino acids aurintricarboxylic acid Biological and medical sciences Biotechnology Cells Coagulation factors ethanolamine ferric sulfate Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Production of active biomolecules prothrombin putrescine Rodents sodium pyruvate Sulfates |
| title | A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T15%3A48%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20low-cost%20chemically%20defined%20protein%20free%20medium%20for%20a%20recombinant%20CHO%20cell%20line%20producing%20prothrombin&rft.jtitle=Biotechnology%20letters&rft.au=ZHAOLIE%20CHEN&rft.date=2000&rft.volume=22&rft.issue=10&rft.spage=837&rft.epage=841&rft.pages=837-841&rft.issn=0141-5492&rft.eissn=1573-6776&rft.coden=BILED3&rft_id=info:doi/10.1023/a:1005665530028&rft_dat=%3Cproquest_pasca%3E17662565%3C/proquest_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=734816853&rft_id=info:pmid/&rfr_iscdi=true |