Extracellular K5 polysaccharidic antigen recovery performed by different cell separation technologies
The K5 polysaccharidic antigen obtainable from a strain of Escherichia coli is the non-sulphated precursor in heparin biosynthesis; K5 is composed by two components, 16000 and 1500 Da, their ratio depending on the fermentation conditions. In this study an assessment was made of how various cell-cult...
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| Veröffentlicht in: | Biotechnology letters 2000-05, Vol.22 (9), p.759-766 |
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| container_title | Biotechnology letters |
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| creator | MANZONI, M BERGOMI, S ROLLINI, M CAVAZZONI, V FELISAZ, C MARINONI, C |
| description | The K5 polysaccharidic antigen obtainable from a strain of Escherichia coli is the non-sulphated precursor in heparin biosynthesis; K5 is composed by two components, 16000 and 1500 Da, their ratio depending on the fermentation conditions. In this study an assessment was made of how various cell-culture filtrate separation technologies affected the components themselves and their ratio. A dynamic membrane filtration technology, a tubular ceramic module for tangential flow microfiltration and the standard discontinuous centrifugation were comparatively employed to perform the separation. Experiments carried out on E. coli cultures containing the two components in different ratios showed that the DMF system is a suitable technology for K5 isolation.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1023/A:1005612405051 |
| format | Article |
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In this study an assessment was made of how various cell-culture filtrate separation technologies affected the components themselves and their ratio. A dynamic membrane filtration technology, a tubular ceramic module for tangential flow microfiltration and the standard discontinuous centrifugation were comparatively employed to perform the separation. Experiments carried out on E. coli cultures containing the two components in different ratios showed that the DMF system is a suitable technology for K5 isolation.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1023/A:1005612405051</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Bacteria ; Biological and medical sciences ; Biosynthesis ; Biotechnology ; Centrifugation ; E coli ; Escherichia coli ; Fermentation ; Filtrate ; Fundamental and applied biological sciences. 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| subjects | Bacteria Biological and medical sciences Biosynthesis Biotechnology Centrifugation E coli Escherichia coli Fermentation Filtrate Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Membrane filtration Microbiology Other active biomolecules polysaccharides Production of active biomolecules |
| title | Extracellular K5 polysaccharidic antigen recovery performed by different cell separation technologies |
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