Isolation and characterization of luminescent bacterium for sludge biodegradation
Microtox is based on the inhibition of luminescence of the bacterium Vibrio fischeri by the toxicants. This technique has been accepted by the USEPA (United States Environmental Protection Agency) as a biomonitoring tool for remediation of toxicants such as hydrocarbon sludge. In the present study,...
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| Veröffentlicht in: | Journal of environmental biology 2015-11, Vol.36 (6), p.1255-1261 |
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| container_title | Journal of environmental biology |
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| creator | Zahaba, Maryam Halmi, Mohd Izuan Effendi Ahmad, Siti Aqlima Shukor, Mohd Yunus Syed, Mohd Arif |
| description | Microtox is based on the inhibition of luminescence of the bacterium Vibrio fischeri by the toxicants. This technique has been accepted by the USEPA (United States Environmental Protection Agency) as a biomonitoring tool for remediation of toxicants such as hydrocarbon sludge. In the present study, a luminescent bacterium was isolated from yellow striped scad (Selaroides leptolepis) and was tentatively identified as Vibrio sp. isolate MZ. This aerobic isolate showed high luminescence activity in a broad range of temperature from 25 to 35 °C. In addition, optimal conditions for high bioluminescence activity in range of pH 7.5 to 8.5 and 10 gl(-1) of sodium chloride, 10 gl(-1) of peptone and 10 gl(-1) of sucrose as carbon source. Bench scale biodegradation 1% sludge (w/v) was set up and degradation was determined using gas chromatography with flame ionised detector (GC-FID). In this study, Rhodococcus sp. strain AQ5NOL2 was used to degrade the sludge. Based on the preliminary results obtained, Vibrio sp. isolate MZwas able to monitor the biodegradation of sludge. Therefore, Vibrio sp. isolate MZ has the potential to be used as a biomonitoring agent for biomonitoring of sludge biodegradation particularly in the tropical ranged environment. |
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This technique has been accepted by the USEPA (United States Environmental Protection Agency) as a biomonitoring tool for remediation of toxicants such as hydrocarbon sludge. In the present study, a luminescent bacterium was isolated from yellow striped scad (Selaroides leptolepis) and was tentatively identified as Vibrio sp. isolate MZ. This aerobic isolate showed high luminescence activity in a broad range of temperature from 25 to 35 °C. In addition, optimal conditions for high bioluminescence activity in range of pH 7.5 to 8.5 and 10 gl(-1) of sodium chloride, 10 gl(-1) of peptone and 10 gl(-1) of sucrose as carbon source. Bench scale biodegradation 1% sludge (w/v) was set up and degradation was determined using gas chromatography with flame ionised detector (GC-FID). In this study, Rhodococcus sp. strain AQ5NOL2 was used to degrade the sludge. Based on the preliminary results obtained, Vibrio sp. isolate MZwas able to monitor the biodegradation of sludge. Therefore, Vibrio sp. isolate MZ has the potential to be used as a biomonitoring agent for biomonitoring of sludge biodegradation particularly in the tropical ranged environment.</description><identifier>ISSN: 0254-8704</identifier><identifier>EISSN: 2394-0379</identifier><identifier>PMID: 26688958</identifier><language>eng</language><publisher>India: Triveni Enterprises</publisher><subject>Animals ; Bacteria - classification ; Bacteria - metabolism ; Biodegradation ; Biodegradation, Environmental ; Bioluminescence ; Biomonitoring ; Bioreactors ; Carbon sources ; Environmental protection ; Environmental science ; Fishes - microbiology ; Gas chromatography ; Luminescence ; Peptones ; Phylogeny ; Rhodococcus ; Selaroides leptolepis ; Sewage ; Sludge ; Sodium chloride ; Toxicants ; Vibrio - genetics ; Vibrio - physiology ; Vibrio fischeri</subject><ispartof>Journal of environmental biology, 2015-11, Vol.36 (6), p.1255-1261</ispartof><rights>Copyright Triveni Enterprises Nov 2015</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26688958$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zahaba, Maryam</creatorcontrib><creatorcontrib>Halmi, Mohd Izuan Effendi</creatorcontrib><creatorcontrib>Ahmad, Siti Aqlima</creatorcontrib><creatorcontrib>Shukor, Mohd Yunus</creatorcontrib><creatorcontrib>Syed, Mohd Arif</creatorcontrib><title>Isolation and characterization of luminescent bacterium for sludge biodegradation</title><title>Journal of environmental biology</title><addtitle>J Environ Biol</addtitle><description>Microtox is based on the inhibition of luminescence of the bacterium Vibrio fischeri by the toxicants. 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This technique has been accepted by the USEPA (United States Environmental Protection Agency) as a biomonitoring tool for remediation of toxicants such as hydrocarbon sludge. In the present study, a luminescent bacterium was isolated from yellow striped scad (Selaroides leptolepis) and was tentatively identified as Vibrio sp. isolate MZ. This aerobic isolate showed high luminescence activity in a broad range of temperature from 25 to 35 °C. In addition, optimal conditions for high bioluminescence activity in range of pH 7.5 to 8.5 and 10 gl(-1) of sodium chloride, 10 gl(-1) of peptone and 10 gl(-1) of sucrose as carbon source. Bench scale biodegradation 1% sludge (w/v) was set up and degradation was determined using gas chromatography with flame ionised detector (GC-FID). In this study, Rhodococcus sp. strain AQ5NOL2 was used to degrade the sludge. Based on the preliminary results obtained, Vibrio sp. isolate MZwas able to monitor the biodegradation of sludge. Therefore, Vibrio sp. isolate MZ has the potential to be used as a biomonitoring agent for biomonitoring of sludge biodegradation particularly in the tropical ranged environment.</abstract><cop>India</cop><pub>Triveni Enterprises</pub><pmid>26688958</pmid><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0254-8704 |
| ispartof | Journal of environmental biology, 2015-11, Vol.36 (6), p.1255-1261 |
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| subjects | Animals Bacteria - classification Bacteria - metabolism Biodegradation Biodegradation, Environmental Bioluminescence Biomonitoring Bioreactors Carbon sources Environmental protection Environmental science Fishes - microbiology Gas chromatography Luminescence Peptones Phylogeny Rhodococcus Selaroides leptolepis Sewage Sludge Sodium chloride Toxicants Vibrio - genetics Vibrio - physiology Vibrio fischeri |
| title | Isolation and characterization of luminescent bacterium for sludge biodegradation |
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