In vitro regeneration through direct shoot organogenesis in honey orange (Citrus tangerina)

Rapid propagation of Honey orange (Citrus tangerina) was achieved by induction of shoots from epicotyl and cotyledonary node explants and rooting of cotyledonary node derived shoots. Significant explant differences were observed in the induction of direct shoots. Cotyledonary node explant is the most efficient in regeneration frequency followed by epicotyl explant. Cotyledonary node explants cultured on Murashige and Skoog (MS) medium supplemented with 8.88 µM N6-benzyladenine (BA) and 0.54 µM α-naphthaleneacetic acid (NAA) developed more than five shoots per explant. The isolated shoots transferred onto the MS medium supplemented with 5.4 µM NAA rooted 100% within 30 days.