Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi
The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including...
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| Veröffentlicht in: | Journal of microbiological methods 2016-02, Vol.121, p.41-43 |
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| container_title | Journal of microbiological methods |
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| creator | Andrews, Megan Y. Santelli, Cara M. Duckworth, Owen W. |
| description | The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including fungi.
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•Modified CAS layer plates have reduced toxicity, making them suitable for fungi.•Method allows visual and quantitative comparison of siderophore exudation.•Siderophore exudation by fungi on CAS layer plates can be quantified as function of time. |
| doi_str_mv | 10.1016/j.mimet.2015.12.012 |
| format | Article |
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[Display omitted]
•Modified CAS layer plates have reduced toxicity, making them suitable for fungi.•Method allows visual and quantitative comparison of siderophore exudation.•Siderophore exudation by fungi on CAS layer plates can be quantified as function of time.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2015.12.012</identifier><identifier>PMID: 26712125</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Agar - chemistry ; CAS assay ; Culture Media ; Fungi ; Fungi - growth & development ; Fungi - metabolism ; Humans ; Hydroxybenzoates - chemistry ; Indicators and Reagents - chemistry ; Microbiological Techniques - methods ; Mycology - methods ; Quantitative assay ; Siderophores ; Siderophores - biosynthesis</subject><ispartof>Journal of microbiological methods, 2016-02, Vol.121, p.41-43</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-7030d6ecb3e21ed003e90514ef3b8792722bc7265a0b51b5b0be44d468b092873</citedby><cites>FETCH-LOGICAL-c437t-7030d6ecb3e21ed003e90514ef3b8792722bc7265a0b51b5b0be44d468b092873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2015.12.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26712125$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Andrews, Megan Y.</creatorcontrib><creatorcontrib>Santelli, Cara M.</creatorcontrib><creatorcontrib>Duckworth, Owen W.</creatorcontrib><title>Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including fungi.
[Display omitted]
•Modified CAS layer plates have reduced toxicity, making them suitable for fungi.•Method allows visual and quantitative comparison of siderophore exudation.•Siderophore exudation by fungi on CAS layer plates can be quantified as function of time.</description><subject>Agar - chemistry</subject><subject>CAS assay</subject><subject>Culture Media</subject><subject>Fungi</subject><subject>Fungi - growth & development</subject><subject>Fungi - metabolism</subject><subject>Humans</subject><subject>Hydroxybenzoates - chemistry</subject><subject>Indicators and Reagents - chemistry</subject><subject>Microbiological Techniques - methods</subject><subject>Mycology - methods</subject><subject>Quantitative assay</subject><subject>Siderophores</subject><subject>Siderophores - biosynthesis</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkclu2zAQQImiQeOk_YICBY-9WOEiidKhB8PIBhjIIcmZ4DJqaFiiTFJB_Pehl-ZY5EQM582CeQj9pKSghNZX66J3PaSCEVoVlBWEsi9oRhvB5g2v2q9olikxF_n_HF3EuCYZ5GXzDZ2zWlBGWTVDryu1g4DHjUqAl4tHrGJUO9z5gNML4O2khuSSSs4P2Hc4OgvBjy8-AB6Dt5M5ZNRgsYUEoXfDBwtvkz0Go0o5N8RD224a_rrv6KxTmwg_Tu8ler65flrezVcPt_fLxWpuSi5S3p0TW4PRHBgFSwiHllS0hI7rRrRMMKaNYHWliK6orjTRUJa2rBtNWtYIfol-H_vmZbcTxCR7Fw1sNmoAP0VJRV21ZUPq8jMoF4Tlc2aUH1ETfIwBOjkG16uwk5TIvRu5lgc3cu9GUiazg1z16zRg0j3Yj5p_MjLw5whAvsirgyCjcTAYsC6ASdJ6998B7_WGocA</recordid><startdate>201602</startdate><enddate>201602</enddate><creator>Andrews, Megan Y.</creator><creator>Santelli, Cara M.</creator><creator>Duckworth, Owen W.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>201602</creationdate><title>Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi</title><author>Andrews, Megan Y. ; Santelli, Cara M. ; Duckworth, Owen W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-7030d6ecb3e21ed003e90514ef3b8792722bc7265a0b51b5b0be44d468b092873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Agar - chemistry</topic><topic>CAS assay</topic><topic>Culture Media</topic><topic>Fungi</topic><topic>Fungi - growth & development</topic><topic>Fungi - metabolism</topic><topic>Humans</topic><topic>Hydroxybenzoates - chemistry</topic><topic>Indicators and Reagents - chemistry</topic><topic>Microbiological Techniques - methods</topic><topic>Mycology - methods</topic><topic>Quantitative assay</topic><topic>Siderophores</topic><topic>Siderophores - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andrews, Megan Y.</creatorcontrib><creatorcontrib>Santelli, Cara M.</creatorcontrib><creatorcontrib>Duckworth, Owen W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andrews, Megan Y.</au><au>Santelli, Cara M.</au><au>Duckworth, Owen W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2016-02</date><risdate>2016</risdate><volume>121</volume><spage>41</spage><epage>43</epage><pages>41-43</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including fungi.
[Display omitted]
•Modified CAS layer plates have reduced toxicity, making them suitable for fungi.•Method allows visual and quantitative comparison of siderophore exudation.•Siderophore exudation by fungi on CAS layer plates can be quantified as function of time.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26712125</pmid><doi>10.1016/j.mimet.2015.12.012</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2016-02, Vol.121, p.41-43 |
| issn | 0167-7012 1872-8359 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agar - chemistry CAS assay Culture Media Fungi Fungi - growth & development Fungi - metabolism Humans Hydroxybenzoates - chemistry Indicators and Reagents - chemistry Microbiological Techniques - methods Mycology - methods Quantitative assay Siderophores Siderophores - biosynthesis |
| title | Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi |
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