A GENETIC MARKER TO SEPARATE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) MORPHOTYPES
Emiliania huxleyi (Lohm.) Hay and Mohler is a ubiquitous unicellular marine alga surrounded by an elaborate covering of calcite platelets called coccoliths. It is an important primary producer involved in oceanic biogeochemistry and climate regulation. Currently, E. huxleyi is separated into five mo...
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| Veröffentlicht in: | Journal of phycology 2005-08, Vol.41 (4), p.874-879 |
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| container_title | Journal of phycology |
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| creator | Schroeder, Declan C. Biggi, Gaia F. Hall, Matthew Davy, Joanne Martínez, Joaquín Martínez Richardson, Anthony J. Malin, Gillian Wilson, William H. |
| description | Emiliania huxleyi (Lohm.) Hay and Mohler is a ubiquitous unicellular marine alga surrounded by an elaborate covering of calcite platelets called coccoliths. It is an important primary producer involved in oceanic biogeochemistry and climate regulation. Currently, E. huxleyi is separated into five morphotypes based on morphometric, physiological, biochemical, and immunological differences. However, a genetic marker has yet to be found to characterize these morphotypes. With the use of sequence analysis and denaturing gradient gel electrophoresis, we discovered a genetic marker that correlates significantly with the separation of the most widely recognized A and B morphotypes. Furthermore, we reveal that the A morphotype is composed of a number of distinct genotypes. This marker lies within the 3' untranslated region of a coccolith associated protein mRNA, which is implicated in regulating coccolith calcification. Consequently, we tentatively termed this marker the coccolith morphology motif. |
| doi_str_mv | 10.1111/j.1529-8817.2005.04188.x |
| format | Article |
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Hay and Mohler is a ubiquitous unicellular marine alga surrounded by an elaborate covering of calcite platelets called coccoliths. It is an important primary producer involved in oceanic biogeochemistry and climate regulation. Currently, E. huxleyi is separated into five morphotypes based on morphometric, physiological, biochemical, and immunological differences. However, a genetic marker has yet to be found to characterize these morphotypes. With the use of sequence analysis and denaturing gradient gel electrophoresis, we discovered a genetic marker that correlates significantly with the separation of the most widely recognized A and B morphotypes. Furthermore, we reveal that the A morphotype is composed of a number of distinct genotypes. This marker lies within the 3' untranslated region of a coccolith associated protein mRNA, which is implicated in regulating coccolith calcification. 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Hay and Mohler is a ubiquitous unicellular marine alga surrounded by an elaborate covering of calcite platelets called coccoliths. It is an important primary producer involved in oceanic biogeochemistry and climate regulation. Currently, E. huxleyi is separated into five morphotypes based on morphometric, physiological, biochemical, and immunological differences. However, a genetic marker has yet to be found to characterize these morphotypes. With the use of sequence analysis and denaturing gradient gel electrophoresis, we discovered a genetic marker that correlates significantly with the separation of the most widely recognized A and B morphotypes. Furthermore, we reveal that the A morphotype is composed of a number of distinct genotypes. This marker lies within the 3' untranslated region of a coccolith associated protein mRNA, which is implicated in regulating coccolith calcification. Consequently, we tentatively termed this marker the coccolith morphology motif.</description><subject>calcification</subject><subject>calcium binding protein</subject><subject>coccolith morphotypes</subject><subject>coccolithophore</subject><subject>coccoliths</subject><subject>Emiliania huxleyi</subject><subject>Marine</subject><issn>0022-3646</issn><issn>1529-8817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNo9T11LwzAADKLgnP6HPIk-tOajTdLHOOJa7Bdtp_YppFsKnZub7Qbz31uYeA93B3ccHAAQIxePeFq72CeBIwTmLkHId5GHhXBPF2DyH1yCCUKEOJR57BrcDMMaIcSZjycgkXCuUlVFM5jI4lUVsMpgqXJZyEpBlURxJNNIwnDxEas6gg95USepKqMsD-uZkuoRJlmRh1lV56q8BVet2Qz27k-nYPGiqlnoxNk8msnY6QjxDk7LV7zhS8QaEggb2JaOruUeoQR7nAkyEmK-ZwNqfb8xomXEN3SJVl5rTMPpFNyfd_f97vtoh4PedsPSbjbmy-6Og8bjOUEJGYvOudgNB3vS-77bmv5Hm_5TM065r9_TuRZvJQ4r-qxL-gueglqm</recordid><startdate>200508</startdate><enddate>200508</enddate><creator>Schroeder, Declan C.</creator><creator>Biggi, Gaia F.</creator><creator>Hall, Matthew</creator><creator>Davy, Joanne</creator><creator>Martínez, Joaquín Martínez</creator><creator>Richardson, Anthony J.</creator><creator>Malin, Gillian</creator><creator>Wilson, William H.</creator><general>Blackwell Science Inc</general><scope>BSCLL</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>200508</creationdate><title>A GENETIC MARKER TO SEPARATE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) MORPHOTYPES</title><author>Schroeder, Declan C. ; Biggi, Gaia F. ; Hall, Matthew ; Davy, Joanne ; Martínez, Joaquín Martínez ; Richardson, Anthony J. ; Malin, Gillian ; Wilson, William H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i224t-f7d7b7c06b298e9ef36b2f742321476824760654e93e55ba8f625a3c0d4faab73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>calcification</topic><topic>calcium binding protein</topic><topic>coccolith morphotypes</topic><topic>coccolithophore</topic><topic>coccoliths</topic><topic>Emiliania huxleyi</topic><topic>Marine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schroeder, Declan C.</creatorcontrib><creatorcontrib>Biggi, Gaia F.</creatorcontrib><creatorcontrib>Hall, Matthew</creatorcontrib><creatorcontrib>Davy, Joanne</creatorcontrib><creatorcontrib>Martínez, Joaquín Martínez</creatorcontrib><creatorcontrib>Richardson, Anthony J.</creatorcontrib><creatorcontrib>Malin, Gillian</creatorcontrib><creatorcontrib>Wilson, William H.</creatorcontrib><collection>Istex</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of phycology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schroeder, Declan C.</au><au>Biggi, Gaia F.</au><au>Hall, Matthew</au><au>Davy, Joanne</au><au>Martínez, Joaquín Martínez</au><au>Richardson, Anthony J.</au><au>Malin, Gillian</au><au>Wilson, William H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A GENETIC MARKER TO SEPARATE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) MORPHOTYPES</atitle><jtitle>Journal of phycology</jtitle><date>2005-08</date><risdate>2005</risdate><volume>41</volume><issue>4</issue><spage>874</spage><epage>879</epage><pages>874-879</pages><issn>0022-3646</issn><eissn>1529-8817</eissn><abstract>Emiliania huxleyi (Lohm.) Hay and Mohler is a ubiquitous unicellular marine alga surrounded by an elaborate covering of calcite platelets called coccoliths. It is an important primary producer involved in oceanic biogeochemistry and climate regulation. Currently, E. huxleyi is separated into five morphotypes based on morphometric, physiological, biochemical, and immunological differences. However, a genetic marker has yet to be found to characterize these morphotypes. With the use of sequence analysis and denaturing gradient gel electrophoresis, we discovered a genetic marker that correlates significantly with the separation of the most widely recognized A and B morphotypes. Furthermore, we reveal that the A morphotype is composed of a number of distinct genotypes. This marker lies within the 3' untranslated region of a coccolith associated protein mRNA, which is implicated in regulating coccolith calcification. Consequently, we tentatively termed this marker the coccolith morphology motif.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Inc</pub><doi>10.1111/j.1529-8817.2005.04188.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of phycology, 2005-08, Vol.41 (4), p.874-879 |
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| language | eng |
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| source | Wiley Online Library All Journals |
| subjects | calcification calcium binding protein coccolith morphotypes coccolithophore coccoliths Emiliania huxleyi Marine |
| title | A GENETIC MARKER TO SEPARATE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) MORPHOTYPES |
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