High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP
By mediating exclusive recombination in selected cell types, mouse strains expressing the site-specific recombinase Cre or FLP add a required, selective dimension to studies of mammalian gene function and cell fate. Having two highly active recombinases would advance such studies and elaborate new a...
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| Veröffentlicht in: | Nature genetics 2000-06, Vol.25 (2), p.139-140 |
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| container_title | Nature genetics |
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| creator | Rodríguez, Carolyn I. Buchholz, Frank Galloway, Jenna Sequerra, Reynaldo Kasper, Jocelyn Ayala, Ramses Stewart, A. Francis Dymecki, Susan M. |
| description | By mediating exclusive recombination in selected cell types, mouse strains expressing the site-specific recombinase Cre or FLP add a required, selective dimension to studies of mammalian gene function and cell fate. Having two highly active recombinases would advance such studies and elaborate new approaches for conditional genetic manipulations. Here we describe deleter mice harbouring FLPe, a recombinase variant isolated by protein evolution to have enhanced thermostability. We show that all FLPe deleter strains achieve maximum target gene excision in both somatic and germ cells, demonstrating that FLPe is highly effective in mice and an important alternative to, and complement for, the Cre-loxP system for in vivo genetic engineering. |
| doi_str_mv | 10.1038/75973 |
| format | Article |
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We show that all FLPe deleter strains achieve maximum target gene excision in both somatic and germ cells, demonstrating that FLPe is highly effective in mice and an important alternative to, and complement for, the Cre-loxP system for in vivo genetic engineering.</description><identifier>ISSN: 1061-4036</identifier><identifier>EISSN: 1546-1718</identifier><identifier>DOI: 10.1038/75973</identifier><identifier>PMID: 10835623</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Agriculture ; Amino Acid Substitution - genetics ; Analysis ; Animal Genetics and Genomics ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; correspondence ; DNA Nucleotidyltransferases - genetics ; DNA Nucleotidyltransferases - metabolism ; Enzyme Stability ; Female ; FLP protein ; Gene Function ; Genes, Reporter - genetics ; Genetic Engineering - methods ; Genetic research ; Human Genetics ; Integrases - genetics ; Integrases - metabolism ; Male ; Mice ; Mice, Transgenic ; Mutagenesis, Site-Directed - genetics ; Organ Specificity ; Physiological aspects ; Recombinases ; Recombination, Genetic - genetics ; Sequence Deletion - genetics ; Transgenes - genetics ; Viral Proteins</subject><ispartof>Nature genetics, 2000-06, Vol.25 (2), p.139-140</ispartof><rights>Nature America Inc. 2000</rights><rights>COPYRIGHT 2000 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jun 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-1c54c23602c0af7cc45812b3157e1806f3bc0dafdedcf63b3e58601b88afc8d53</citedby><cites>FETCH-LOGICAL-c469t-1c54c23602c0af7cc45812b3157e1806f3bc0dafdedcf63b3e58601b88afc8d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/75973$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/75973$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10835623$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodríguez, Carolyn I.</creatorcontrib><creatorcontrib>Buchholz, Frank</creatorcontrib><creatorcontrib>Galloway, Jenna</creatorcontrib><creatorcontrib>Sequerra, Reynaldo</creatorcontrib><creatorcontrib>Kasper, Jocelyn</creatorcontrib><creatorcontrib>Ayala, Ramses</creatorcontrib><creatorcontrib>Stewart, A. Francis</creatorcontrib><creatorcontrib>Dymecki, Susan M.</creatorcontrib><title>High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP</title><title>Nature genetics</title><addtitle>Nat Genet</addtitle><addtitle>Nat Genet</addtitle><description>By mediating exclusive recombination in selected cell types, mouse strains expressing the site-specific recombinase Cre or FLP add a required, selective dimension to studies of mammalian gene function and cell fate. Having two highly active recombinases would advance such studies and elaborate new approaches for conditional genetic manipulations. Here we describe deleter mice harbouring FLPe, a recombinase variant isolated by protein evolution to have enhanced thermostability. 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| identifier | ISSN: 1061-4036 |
| ispartof | Nature genetics, 2000-06, Vol.25 (2), p.139-140 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | Agriculture Amino Acid Substitution - genetics Analysis Animal Genetics and Genomics Animals Biomedical and Life Sciences Biomedicine Cancer Research correspondence DNA Nucleotidyltransferases - genetics DNA Nucleotidyltransferases - metabolism Enzyme Stability Female FLP protein Gene Function Genes, Reporter - genetics Genetic Engineering - methods Genetic research Human Genetics Integrases - genetics Integrases - metabolism Male Mice Mice, Transgenic Mutagenesis, Site-Directed - genetics Organ Specificity Physiological aspects Recombinases Recombination, Genetic - genetics Sequence Deletion - genetics Transgenes - genetics Viral Proteins |
| title | High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP |
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