Assessment of liver fibrosis in primary biliary cholangitis: Comparison between indirect serum markers and fibrosis morphometry

Abstract Background The accuracy of non-invasive methods for the quantification of liver fibrosis in primary biliary cholangitis (PBC) is still debated. Aims To determine the histo-morphometric measurement of fibrotic tissue and to explore the possible association between indirect markers (APRI, FOR...

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Veröffentlicht in:Digestive and liver disease 2016-03, Vol.48 (3), p.298-301
Hauptverfasser: Stasi, Cristina, Leoncini, Luisa, Biagini, Maria Rosa, Arena, Umberto, Madiai, Stefania, Laffi, Giacomo, Marra, Fabio, Milani, Stefano
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container_end_page 301
container_issue 3
container_start_page 298
container_title Digestive and liver disease
container_volume 48
creator Stasi, Cristina
Leoncini, Luisa
Biagini, Maria Rosa
Arena, Umberto
Madiai, Stefania
Laffi, Giacomo
Marra, Fabio
Milani, Stefano
description Abstract Background The accuracy of non-invasive methods for the quantification of liver fibrosis in primary biliary cholangitis (PBC) is still debated. Aims To determine the histo-morphometric measurement of fibrotic tissue and to explore the possible association between indirect markers (APRI, FORNS, FIB-4, and Lok) and morphometry. Methods Retrospective analysis of medical data from patients with PBC, on whom needle liver biopsy was performed as part of the diagnostic assessment. One section of each biopsy stained with Sirius red was used for calculating the percentage of collagen. Quantitative measure of fibrotic tissue (fibrosis morphometry) was calculated as a percentage of collagen content by digital image analysis. Morphometry results were divided into four groups reflecting Ludwig's staging and compared with values for indirect serum markers. Results 50 PBC patients were enrolled (86% females, mean age 57 ± 12.30 years), 19 were Ludwig's stage I (38%), 14 stage II (28%), 12 stage III (24%), and five stage IV (10%). Morphometry results were significantly different among Ludwig stages ( p < 0.05). No significant differences were found for indirect serum markers. A significant correlation was found between morphometry results and indirect serum markers tested ( p < 0.05). Conclusion In our cohort, the histo-morphometric values of fibrotic tissue increased progressively with Ludwig's stages of PBC, while non-invasive markers did not.
doi_str_mv 10.1016/j.dld.2015.10.024
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Aims To determine the histo-morphometric measurement of fibrotic tissue and to explore the possible association between indirect markers (APRI, FORNS, FIB-4, and Lok) and morphometry. Methods Retrospective analysis of medical data from patients with PBC, on whom needle liver biopsy was performed as part of the diagnostic assessment. One section of each biopsy stained with Sirius red was used for calculating the percentage of collagen. Quantitative measure of fibrotic tissue (fibrosis morphometry) was calculated as a percentage of collagen content by digital image analysis. Morphometry results were divided into four groups reflecting Ludwig's staging and compared with values for indirect serum markers. Results 50 PBC patients were enrolled (86% females, mean age 57 ± 12.30 years), 19 were Ludwig's stage I (38%), 14 stage II (28%), 12 stage III (24%), and five stage IV (10%). Morphometry results were significantly different among Ludwig stages ( p &lt; 0.05). No significant differences were found for indirect serum markers. A significant correlation was found between morphometry results and indirect serum markers tested ( p &lt; 0.05). Conclusion In our cohort, the histo-morphometric values of fibrotic tissue increased progressively with Ludwig's stages of PBC, while non-invasive markers did not.</description><identifier>ISSN: 1590-8658</identifier><identifier>EISSN: 1878-3562</identifier><identifier>DOI: 10.1016/j.dld.2015.10.024</identifier><identifier>PMID: 26632448</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Aged ; Alanine Transaminase - blood ; Alkaline Phosphatase - blood ; Aspartate Aminotransferases - blood ; Biomarkers - blood ; Biopsy ; Biopsy, Needle ; Cohort Studies ; Disease Progression ; Female ; Fibrosis ; gamma-Glutamyltransferase - blood ; Gastroenterology and Hepatology ; Humans ; Image Processing, Computer-Assisted ; Indirect serum markers ; Liver - pathology ; Liver Cirrhosis, Biliary - blood ; Liver Cirrhosis, Biliary - pathology ; Ludwig's stages ; Male ; Middle Aged ; Morphometry ; Primary biliary cirrhosis ; Retrospective Studies</subject><ispartof>Digestive and liver disease, 2016-03, Vol.48 (3), p.298-301</ispartof><rights>Editrice Gastroenterologica Italiana S.r.l.</rights><rights>2015 Editrice Gastroenterologica Italiana S.r.l.</rights><rights>Copyright © 2015 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-1151dc223322a6c5d2c59416dff6abd24909e14025198839250168cf7eaafe483</citedby><cites>FETCH-LOGICAL-c408t-1151dc223322a6c5d2c59416dff6abd24909e14025198839250168cf7eaafe483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dld.2015.10.024$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26632448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stasi, Cristina</creatorcontrib><creatorcontrib>Leoncini, Luisa</creatorcontrib><creatorcontrib>Biagini, Maria Rosa</creatorcontrib><creatorcontrib>Arena, Umberto</creatorcontrib><creatorcontrib>Madiai, Stefania</creatorcontrib><creatorcontrib>Laffi, Giacomo</creatorcontrib><creatorcontrib>Marra, Fabio</creatorcontrib><creatorcontrib>Milani, Stefano</creatorcontrib><title>Assessment of liver fibrosis in primary biliary cholangitis: Comparison between indirect serum markers and fibrosis morphometry</title><title>Digestive and liver disease</title><addtitle>Dig Liver Dis</addtitle><description>Abstract Background The accuracy of non-invasive methods for the quantification of liver fibrosis in primary biliary cholangitis (PBC) is still debated. Aims To determine the histo-morphometric measurement of fibrotic tissue and to explore the possible association between indirect markers (APRI, FORNS, FIB-4, and Lok) and morphometry. Methods Retrospective analysis of medical data from patients with PBC, on whom needle liver biopsy was performed as part of the diagnostic assessment. One section of each biopsy stained with Sirius red was used for calculating the percentage of collagen. Quantitative measure of fibrotic tissue (fibrosis morphometry) was calculated as a percentage of collagen content by digital image analysis. Morphometry results were divided into four groups reflecting Ludwig's staging and compared with values for indirect serum markers. Results 50 PBC patients were enrolled (86% females, mean age 57 ± 12.30 years), 19 were Ludwig's stage I (38%), 14 stage II (28%), 12 stage III (24%), and five stage IV (10%). Morphometry results were significantly different among Ludwig stages ( p &lt; 0.05). No significant differences were found for indirect serum markers. A significant correlation was found between morphometry results and indirect serum markers tested ( p &lt; 0.05). 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Leoncini, Luisa ; Biagini, Maria Rosa ; Arena, Umberto ; Madiai, Stefania ; Laffi, Giacomo ; Marra, Fabio ; Milani, Stefano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-1151dc223322a6c5d2c59416dff6abd24909e14025198839250168cf7eaafe483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aged</topic><topic>Alanine Transaminase - blood</topic><topic>Alkaline Phosphatase - blood</topic><topic>Aspartate Aminotransferases - blood</topic><topic>Biomarkers - blood</topic><topic>Biopsy</topic><topic>Biopsy, Needle</topic><topic>Cohort Studies</topic><topic>Disease Progression</topic><topic>Female</topic><topic>Fibrosis</topic><topic>gamma-Glutamyltransferase - blood</topic><topic>Gastroenterology and Hepatology</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted</topic><topic>Indirect serum markers</topic><topic>Liver - pathology</topic><topic>Liver Cirrhosis, Biliary - blood</topic><topic>Liver Cirrhosis, Biliary - pathology</topic><topic>Ludwig's stages</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Morphometry</topic><topic>Primary biliary cirrhosis</topic><topic>Retrospective Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stasi, Cristina</creatorcontrib><creatorcontrib>Leoncini, Luisa</creatorcontrib><creatorcontrib>Biagini, Maria Rosa</creatorcontrib><creatorcontrib>Arena, Umberto</creatorcontrib><creatorcontrib>Madiai, Stefania</creatorcontrib><creatorcontrib>Laffi, Giacomo</creatorcontrib><creatorcontrib>Marra, Fabio</creatorcontrib><creatorcontrib>Milani, Stefano</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Digestive and liver disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stasi, Cristina</au><au>Leoncini, Luisa</au><au>Biagini, Maria Rosa</au><au>Arena, Umberto</au><au>Madiai, Stefania</au><au>Laffi, Giacomo</au><au>Marra, Fabio</au><au>Milani, Stefano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of liver fibrosis in primary biliary cholangitis: Comparison between indirect serum markers and fibrosis morphometry</atitle><jtitle>Digestive and liver disease</jtitle><addtitle>Dig Liver Dis</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>48</volume><issue>3</issue><spage>298</spage><epage>301</epage><pages>298-301</pages><issn>1590-8658</issn><eissn>1878-3562</eissn><abstract>Abstract Background The accuracy of non-invasive methods for the quantification of liver fibrosis in primary biliary cholangitis (PBC) is still debated. Aims To determine the histo-morphometric measurement of fibrotic tissue and to explore the possible association between indirect markers (APRI, FORNS, FIB-4, and Lok) and morphometry. Methods Retrospective analysis of medical data from patients with PBC, on whom needle liver biopsy was performed as part of the diagnostic assessment. One section of each biopsy stained with Sirius red was used for calculating the percentage of collagen. Quantitative measure of fibrotic tissue (fibrosis morphometry) was calculated as a percentage of collagen content by digital image analysis. Morphometry results were divided into four groups reflecting Ludwig's staging and compared with values for indirect serum markers. Results 50 PBC patients were enrolled (86% females, mean age 57 ± 12.30 years), 19 were Ludwig's stage I (38%), 14 stage II (28%), 12 stage III (24%), and five stage IV (10%). Morphometry results were significantly different among Ludwig stages ( p &lt; 0.05). No significant differences were found for indirect serum markers. A significant correlation was found between morphometry results and indirect serum markers tested ( p &lt; 0.05). Conclusion In our cohort, the histo-morphometric values of fibrotic tissue increased progressively with Ludwig's stages of PBC, while non-invasive markers did not.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>26632448</pmid><doi>10.1016/j.dld.2015.10.024</doi><tpages>4</tpages></addata></record>
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subjects Aged
Alanine Transaminase - blood
Alkaline Phosphatase - blood
Aspartate Aminotransferases - blood
Biomarkers - blood
Biopsy
Biopsy, Needle
Cohort Studies
Disease Progression
Female
Fibrosis
gamma-Glutamyltransferase - blood
Gastroenterology and Hepatology
Humans
Image Processing, Computer-Assisted
Indirect serum markers
Liver - pathology
Liver Cirrhosis, Biliary - blood
Liver Cirrhosis, Biliary - pathology
Ludwig's stages
Male
Middle Aged
Morphometry
Primary biliary cirrhosis
Retrospective Studies
title Assessment of liver fibrosis in primary biliary cholangitis: Comparison between indirect serum markers and fibrosis morphometry
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