Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis
Objectives MicroRNA-200 family (miR-200f) has been consistently reported to be deregulated and modulate the metastatic process in multiple cancers. In the present study, we detected the expression of miR-200f in breast cancer (BC) tissue and explored its relationships with clinicopathological charac...
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| Veröffentlicht in: | Clinical & translational oncology 2016-03, Vol.18 (3), p.283-288 |
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| container_title | Clinical & translational oncology |
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| creator | Xu, Feng He, Hua Huang, Wen Lin, Yunting Luo, Shiyu Du, Qian Duan, Ranhui |
| description | Objectives
MicroRNA-200 family (miR-200f) has been consistently reported to be deregulated and modulate the metastatic process in multiple cancers. In the present study, we detected the expression of miR-200f in breast cancer (BC) tissue and explored its relationships with clinicopathological characteristics, especially with lymph node metastasis.
Methods
Expression levels of miR-200a, miR-200b, miR-200c, miR-141, and miR-429 in 99 pairs of BC tissues and adjacent normal tissues were measured by real-time quantitative PCR. The correlation between miR-200f level and multiple clinicopathological factors was then examined by Mann–Whitney test, ANOVA, and operating characteristic (ROC) analysis.
Results
All members of the miR-200f were down-regulated in BC tissue compared with that in normal adjacent tissue; miR-200a, miR-200b, and miR-200c were highly decreased (
p
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| doi_str_mv | 10.1007/s12094-015-1364-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1765112285</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1765112285</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-a20232334b7b524113f3caba237df8df163e3e224e0fb0e4210e9f70cab374283</originalsourceid><addsrcrecordid>eNp9kE1vFDEMhiMEou3CD-CCcuQyYDuZmd1jVb4qtVRCIHGLMjMOmzIfSzwjuv-erHbh2Esc2Y9fyY9SrxDeIkD9TpBgYwvAskBT2QKfqHOsNpvCQFk-Pf3Brn-cqQuRe8jdCvG5OqOKAC2V5-rXe24Te-FO88MusUicRj0FfRvbNH39clkQgA5-iP1ex1Fvl8GPujmszLr1Y8tJR9FeZGqjn3PMnzhvdb8fdls9Th3rgefMeonyQj0Lvhd-eaor9f3jh29Xn4ubu0_XV5c3RWusnQtPQIaMsU3dlGQRTTCtbzyZugvrLmBl2DCRZQgNsCUE3oQaMmNqS2uzUm-Oubs0_V5YZjdEabnv_cjTIg7rqkQkWpcZxSOajxVJHNwuxcGnvUNwB8fu6Nhlx-7gOD8r9foUvzQDd_83_knNAB0ByaPxJyd3Py1pzCc_kvoXyIuGyw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1765112285</pqid></control><display><type>article</type><title>Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Xu, Feng ; He, Hua ; Huang, Wen ; Lin, Yunting ; Luo, Shiyu ; Du, Qian ; Duan, Ranhui</creator><creatorcontrib>Xu, Feng ; He, Hua ; Huang, Wen ; Lin, Yunting ; Luo, Shiyu ; Du, Qian ; Duan, Ranhui</creatorcontrib><description>Objectives
MicroRNA-200 family (miR-200f) has been consistently reported to be deregulated and modulate the metastatic process in multiple cancers. In the present study, we detected the expression of miR-200f in breast cancer (BC) tissue and explored its relationships with clinicopathological characteristics, especially with lymph node metastasis.
Methods
Expression levels of miR-200a, miR-200b, miR-200c, miR-141, and miR-429 in 99 pairs of BC tissues and adjacent normal tissues were measured by real-time quantitative PCR. The correlation between miR-200f level and multiple clinicopathological factors was then examined by Mann–Whitney test, ANOVA, and operating characteristic (ROC) analysis.
Results
All members of the miR-200f were down-regulated in BC tissue compared with that in normal adjacent tissue; miR-200a, miR-200b, and miR-200c were highly decreased (
p
< 0.05), while the differences of miR-141 and miR-429 between patients and the control group were not statistically significant. Furthermore, all five members were found to be distinctly decreased with the incidence of lymph node metastasis (
p
< 0.05); When the patients were divided into three groups according to the number of lymph node metastasis (0; 1–3; ≥4), a gradual decrease of miR-200f expression was observed with the increasing number of lymph node metastasis; ROC revealed that miR-200b can differentiate patients with lymph node metastasis from those without lymph node metastasis.
Conclusion
These observations imply that the down-regulation of miR-200f in human BC is associated with an invasive phenotype, and miR-200b may be useful to estimate the likelihood of the presence of pathologically positive lymph nodes.</description><identifier>ISSN: 1699-048X</identifier><identifier>EISSN: 1699-3055</identifier><identifier>DOI: 10.1007/s12094-015-1364-1</identifier><identifier>PMID: 26201425</identifier><language>eng</language><publisher>Milan: Springer Milan</publisher><subject>Adult ; Area Under Curve ; Biomarkers, Tumor - genetics ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Carcinoma, Ductal, Breast - genetics ; Carcinoma, Ductal, Breast - pathology ; Down-Regulation ; Female ; Humans ; Lymphatic Metastasis ; Medicine ; Medicine & Public Health ; MicroRNAs - genetics ; Middle Aged ; Oncology ; Real-Time Polymerase Chain Reaction ; Research Article ; ROC Curve</subject><ispartof>Clinical & translational oncology, 2016-03, Vol.18 (3), p.283-288</ispartof><rights>Federación de Sociedades Españolas de Oncología (FESEO) 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-a20232334b7b524113f3caba237df8df163e3e224e0fb0e4210e9f70cab374283</citedby><cites>FETCH-LOGICAL-c344t-a20232334b7b524113f3caba237df8df163e3e224e0fb0e4210e9f70cab374283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12094-015-1364-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12094-015-1364-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26201425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Feng</creatorcontrib><creatorcontrib>He, Hua</creatorcontrib><creatorcontrib>Huang, Wen</creatorcontrib><creatorcontrib>Lin, Yunting</creatorcontrib><creatorcontrib>Luo, Shiyu</creatorcontrib><creatorcontrib>Du, Qian</creatorcontrib><creatorcontrib>Duan, Ranhui</creatorcontrib><title>Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis</title><title>Clinical & translational oncology</title><addtitle>Clin Transl Oncol</addtitle><addtitle>Clin Transl Oncol</addtitle><description>Objectives
MicroRNA-200 family (miR-200f) has been consistently reported to be deregulated and modulate the metastatic process in multiple cancers. In the present study, we detected the expression of miR-200f in breast cancer (BC) tissue and explored its relationships with clinicopathological characteristics, especially with lymph node metastasis.
Methods
Expression levels of miR-200a, miR-200b, miR-200c, miR-141, and miR-429 in 99 pairs of BC tissues and adjacent normal tissues were measured by real-time quantitative PCR. The correlation between miR-200f level and multiple clinicopathological factors was then examined by Mann–Whitney test, ANOVA, and operating characteristic (ROC) analysis.
Results
All members of the miR-200f were down-regulated in BC tissue compared with that in normal adjacent tissue; miR-200a, miR-200b, and miR-200c were highly decreased (
p
< 0.05), while the differences of miR-141 and miR-429 between patients and the control group were not statistically significant. Furthermore, all five members were found to be distinctly decreased with the incidence of lymph node metastasis (
p
< 0.05); When the patients were divided into three groups according to the number of lymph node metastasis (0; 1–3; ≥4), a gradual decrease of miR-200f expression was observed with the increasing number of lymph node metastasis; ROC revealed that miR-200b can differentiate patients with lymph node metastasis from those without lymph node metastasis.
Conclusion
These observations imply that the down-regulation of miR-200f in human BC is associated with an invasive phenotype, and miR-200b may be useful to estimate the likelihood of the presence of pathologically positive lymph nodes.</description><subject>Adult</subject><subject>Area Under Curve</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - pathology</subject><subject>Carcinoma, Ductal, Breast - genetics</subject><subject>Carcinoma, Ductal, Breast - pathology</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Humans</subject><subject>Lymphatic Metastasis</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>Oncology</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Research Article</subject><subject>ROC Curve</subject><issn>1699-048X</issn><issn>1699-3055</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1vFDEMhiMEou3CD-CCcuQyYDuZmd1jVb4qtVRCIHGLMjMOmzIfSzwjuv-erHbh2Esc2Y9fyY9SrxDeIkD9TpBgYwvAskBT2QKfqHOsNpvCQFk-Pf3Brn-cqQuRe8jdCvG5OqOKAC2V5-rXe24Te-FO88MusUicRj0FfRvbNH39clkQgA5-iP1ex1Fvl8GPujmszLr1Y8tJR9FeZGqjn3PMnzhvdb8fdls9Th3rgefMeonyQj0Lvhd-eaor9f3jh29Xn4ubu0_XV5c3RWusnQtPQIaMsU3dlGQRTTCtbzyZugvrLmBl2DCRZQgNsCUE3oQaMmNqS2uzUm-Oubs0_V5YZjdEabnv_cjTIg7rqkQkWpcZxSOajxVJHNwuxcGnvUNwB8fu6Nhlx-7gOD8r9foUvzQDd_83_knNAB0ByaPxJyd3Py1pzCc_kvoXyIuGyw</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Xu, Feng</creator><creator>He, Hua</creator><creator>Huang, Wen</creator><creator>Lin, Yunting</creator><creator>Luo, Shiyu</creator><creator>Du, Qian</creator><creator>Duan, Ranhui</creator><general>Springer Milan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160301</creationdate><title>Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis</title><author>Xu, Feng ; He, Hua ; Huang, Wen ; Lin, Yunting ; Luo, Shiyu ; Du, Qian ; Duan, Ranhui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-a20232334b7b524113f3caba237df8df163e3e224e0fb0e4210e9f70cab374283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Area Under Curve</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - pathology</topic><topic>Carcinoma, Ductal, Breast - genetics</topic><topic>Carcinoma, Ductal, Breast - pathology</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Humans</topic><topic>Lymphatic Metastasis</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><topic>Oncology</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Research Article</topic><topic>ROC Curve</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Feng</creatorcontrib><creatorcontrib>He, Hua</creatorcontrib><creatorcontrib>Huang, Wen</creatorcontrib><creatorcontrib>Lin, Yunting</creatorcontrib><creatorcontrib>Luo, Shiyu</creatorcontrib><creatorcontrib>Du, Qian</creatorcontrib><creatorcontrib>Duan, Ranhui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical & translational oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Feng</au><au>He, Hua</au><au>Huang, Wen</au><au>Lin, Yunting</au><au>Luo, Shiyu</au><au>Du, Qian</au><au>Duan, Ranhui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis</atitle><jtitle>Clinical & translational oncology</jtitle><stitle>Clin Transl Oncol</stitle><addtitle>Clin Transl Oncol</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>18</volume><issue>3</issue><spage>283</spage><epage>288</epage><pages>283-288</pages><issn>1699-048X</issn><eissn>1699-3055</eissn><abstract>Objectives
MicroRNA-200 family (miR-200f) has been consistently reported to be deregulated and modulate the metastatic process in multiple cancers. In the present study, we detected the expression of miR-200f in breast cancer (BC) tissue and explored its relationships with clinicopathological characteristics, especially with lymph node metastasis.
Methods
Expression levels of miR-200a, miR-200b, miR-200c, miR-141, and miR-429 in 99 pairs of BC tissues and adjacent normal tissues were measured by real-time quantitative PCR. The correlation between miR-200f level and multiple clinicopathological factors was then examined by Mann–Whitney test, ANOVA, and operating characteristic (ROC) analysis.
Results
All members of the miR-200f were down-regulated in BC tissue compared with that in normal adjacent tissue; miR-200a, miR-200b, and miR-200c were highly decreased (
p
< 0.05), while the differences of miR-141 and miR-429 between patients and the control group were not statistically significant. Furthermore, all five members were found to be distinctly decreased with the incidence of lymph node metastasis (
p
< 0.05); When the patients were divided into three groups according to the number of lymph node metastasis (0; 1–3; ≥4), a gradual decrease of miR-200f expression was observed with the increasing number of lymph node metastasis; ROC revealed that miR-200b can differentiate patients with lymph node metastasis from those without lymph node metastasis.
Conclusion
These observations imply that the down-regulation of miR-200f in human BC is associated with an invasive phenotype, and miR-200b may be useful to estimate the likelihood of the presence of pathologically positive lymph nodes.</abstract><cop>Milan</cop><pub>Springer Milan</pub><pmid>26201425</pmid><doi>10.1007/s12094-015-1364-1</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Area Under Curve Biomarkers, Tumor - genetics Breast Neoplasms - genetics Breast Neoplasms - pathology Carcinoma, Ductal, Breast - genetics Carcinoma, Ductal, Breast - pathology Down-Regulation Female Humans Lymphatic Metastasis Medicine Medicine & Public Health MicroRNAs - genetics Middle Aged Oncology Real-Time Polymerase Chain Reaction Research Article ROC Curve |
| title | Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis |
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